Airways Biology Initiative Summer Internship Experience

Summer of 2013

Kevin TruskowskiI began working at ABI in the spring of 2013 as my first and only Drexel co-op. As someone who is interested in graduate school and in doing research as a career, I think my decision to work at ABI was the best decision I could have made. Here, I was treated like part of the lab, not just as a set of hands. I was encouraged to think like a scientist and was able to discuss my own ideas for experiments relating to what interested me. I think this is something very unique in a co-op. I was allowed to have my own ideas and those ideas were actually taken seriously. By about half way through my time here I was given my own project to look into. It was something our lab had not looked into yet, so I was able to really dive into the literature and learn what I needed to learn to design my own experiments, something I had never had the opportunity to do before. I think this is something quite unique; most labs will lay out experiments for an undergrad to carry out, but here (with some help of course) I was really encouraged to do my own thing.

In the lab I got the chance to work with incredibly intelligent, experienced scientists, both M.D.s and P.h.Ds, from many different backgrounds. They are all experts at what they do and I was fortunate to learn different assays and techniques from them that I wouldn’t have found anywhere else. I was able to see all of the different ways they think and go about trying to answer questions which definitely helped me to grow as a scientist. Despite their experience and intelligence, they were all super friendly and were always willing to discuss any questions I had. The experience and knowledge I’ve been able to gain here has far exceeded my expectations.

 

John GowI entered into the Airways Biology Initiative’s summer research program upon the conclusion of my junior year of high school. I was looking for a way to fortify what I had learned in my AP Chemistry course and increase my knowledge in the realm of respiratory illness, a topic I might be interested in pursuing as a career. During the start of my work in the lab I came to realize what I was doing was very different from the rudiments of the classroom; instead of learning in theory I was discovering with hands on research, under the guidance of knowledgeable scientists. Throughout the summer I was under the supervision of Dr. Atochina-Vasserman, in the Krymskaya lab, and researched a rare respiratory illness called Lymphangioleiomyomatosis by performing SP-A and SP-D western blots, turning frozen lung into homogenate, counting cells, and preforming BCA protein and ELISA assays. My first research experience in a scientific laboratory has taught me a plethora of techniques that I would never have experienced in my high school and all from a friendly knowledgeable group of scientists. Going to the laboratory has shown me the way that science is done in the real world with constant trial and error and frequent repetition of experiments to strengthen conclusions about never before answered questions.

What makes ABI unique is the open atmosphere in which they operate. The scientist are always willing to assist and mentor, but are also willing to step back and let you run your own experiments. Overall ABI is the best thing that I could have done this summer, because it gave me distinctive and valuable experience in the researching field and all in a great atmosphere. I would recommend anyone who is looking to learn about research in the scientific community to take advantage of the remarkable opportunity at ABI.

John’s hard work during the summer was recognized nationally, as he was selected as, one of one hundred regional finalists to compete in the 2013 Siemens Competition in Math, Science & Technology.

 

Aakanksh JaikumarIn the summer before my senior year of high school, I was fortunate enough to participate in the Airways Biology Initiative’s Summer Research Program. This was my first experience working in a scientific laboratory. This amazing opportunity allowed me to learn a multitude of general yet necessary lab techniques from a very friendly, supportive and knowledgeable group of mentors. Unlike learning in a classroom, I was able to get hands-on experience by performing several procedures such as RNA extraction, ozone exposure, feeding and stock media preparation, western blotting, BCA protein assay, cell-counting and data analysis with Microsoft Excel. In addition, I was able to observe many other advanced techniques and procedures performed throughout the lab. This program is a great introduction to translational research for students interested in a career in science.

Overall, I thoroughly enjoyed the program at ABI. The opportunity to work with such a great team of professionals while gaining a remarkable amount of knowledge and experience was definitely positive and worthwhile.

 

Guovanna ShoukriI entered ABI as part of the Drexel University cooperative education program under the guidance of Dr. Vera Krymskaya. During the start of my internship in the laboratory I was quite overwhelmed despite my previous experience with research. I learned that clinical research can be very different from academic research and that a good work ethic and dedication are two of the most important driving forces needed to achieve success. Within my first two weeks as a member of the laboratory, I was asked to study the project that I would be dedicated to for the summer and learned all of the techniques that would help me to do so. Much of my work included cell line maintenance and harvesting, as well as, measuring protein levels and expression through the use of Western blotting and ELISA. Additionally, I had the opportunity to participate in animal work with our mouse models. Through this experience I took part in experiments involving tail vein injections of the mice with tumorigenic cells, drug treatments and clinical trials, as well as, DNA extraction and genotyping of the mice. My work in the laboratory varied, but one thing remained consistent: there was always an opportunity to learn.

If there is one thing that I was able to take away from this experience is that with the ambition to learn and advance in the fields of science and research, many opportunities are possible. And with the mentorship and guidance I received I was afforded a unique and valuable experience.

 

Michael PhanMy second summer at ABI was even more enjoyable than the first. A lot of the same people were still there which made coming back much easier. The best change I’ve noticed between my first and second time is how well they are interfacing with the summer students. This was great, because now they get each individual students up to speed at a much faster and more efficient manner and as a result they now have them doing research in no time. The time between orientation and beginning of actual learning and performing experiments was almost nonexistent.

Here at ABI they were very good with allowing me to explore techniques that fascinated me. You can approach any of the investigators about your interests and they will help guide you in the right direction and show you how to set up experiments utilizing the techniques that interested you. But the best part of ABI is how relevant the research that you perform is. I have had the opportunity to work on studies that have been applicable to the lab’s publications and grants.

 

Sabrina PogrebivskyThe summer after my sophomore year in high school I worked as an intern for the Airways Biology Initiative (ABI) at University of Pennsylvania. Under the supervision of Dr. Elena Atochina-Vasserman, from the Dr. Vera Krymskaya lab, I have learned a lot these past few months. Every day at ABI was a new experience. I focused on a rare genetic lung cancer called Lymphangioleiomyomatosis (LAM), which mainly affects young women by destroying their lung tissue. Two months ago I was not aware this disease existed, but today I know a lot about it. Dr. Atochina-Vasserman’s research involved tests of two different drugs on mice with LAM and whether or not they slow down the progression of lesion growth. I was responsible for calculating data collected in the lab using Excel, GraphPad Prism, and PowerPoint. I learned how to analyze the scientific results of a BCA Protein Assay, qRT PCR and ELISA, and statistical differences between the treatment groups and made graphs to be presented at lab meetings. I created a summary table for the entire experiment of 80 mice, which demonstrated the magnitude of all the data collected during the past 2 months. I learned how to do a search of scientific articles on the Internet and attended scientific lectures. Additionally I got a tour of the entire campus and visited other labs, including the Institute for Environmental Medicine with the historical hyperbaric chambers and laboratories in UPENN’s new Smilow Research Center. I had the opportunity to meet and speak to a lot of interesting scientific professionals who were happy to answer my questions and share their knowledge about research.

Now that the ABI summer research program is almost over, I have experience in biomedical engineering, which I have always been thinking of as my future career. I loved coming into the lab every day and being around very smart people who were all dedicated their time to find the cure for diseases. My experiences at ABI have taught me not only about biology, but also about having a career that can help others. I highly recommend it to anyone who is interested in gaining experience in the lab and pursuing a career in biology or medicine.

 

Andrew ParambathBefore going into college, I had the opportunity to be an intern at ABI, Airway Biology Initiative, at the University of Pennsylvania. I was really fortunate because in high school you have limited exposure to the lab. However, at ABI you are able to learn and practice lab techniques that you would never have the opportunity to in high school. One of the most remarkable experiences I had at ABI was observing Dr. Koziol-White work with an actual human lung. In addition to observing, I had the opportunity to perform several different experiments such as exposing cell lines and lung samples to ozone, analyzing calcium data, and preparing media. Furthermore, as an intern, I was given the independence and responsibility to do things on my own, however, there was always someone with the knowledge and experience I could turn to if I needed help. On top of that, the staff at ABI was very friendly and approachable with any question or concern that I had.

As a result of my experience at ABI, I have learned and implemented various lab techniques, gained valuable connections with the staff and students of ABI, and strengthened my decision to pursue a career in the field of medicine. I would recommend this program to any student interested in medicine or biomedical research. If a student is looking for a rewarding and enlightening experience during the summer then ABI is the place to be!

 

Monica SoniThis past summer, I had the opportunity to intern at the Airways Biology Initiative as a summer researcher. This was my first experience conducting bench research, so my main goals for the summer were to learn several basic laboratory techniques and to gain a hands-on experience in order to gauge my interest in further pursuing bench research. And, after this internship, I can truly say that I have met these goals and more!

I had the privilege of being part of the Haczku lab and had some wonderful mentors who patiently taught me how to perform BCA protein assays, western blots, differential cells counts, cytospins, and cell cultures, as well as how to use a pipette properly. I have also learned how to use Excel effectively for analyzing and presenting data, which was necessary for weekly lab meetings. Additionally, I attended a hands-on mouse training class and, thus, was able to assist with experiments involving live mice, such as lung function tests and genotyping.

In addition to my lab experience, I participated in the Penn Undergraduate Environmental Health Scholars Program (STEER), which exposed me to many public and environmental health issues in Philadelphia through lectures and field trips. This was also a very valuable experience and culminated in a final summer research presentation. Overall, I had a wonderful research experience at the ABI and I am very thankful to all in the Haczku lab for their support and mentorship.

 

Stephane GuillouAs a student from Drexel University, the Airways Biology Initiative (ABI) really stood out to me due to having friends whom had positive experiences at the laboratory and its close proximity to campus. Also, my previous co-ops were more geared towards engineering, so I knew ABI would provide me with a new and completely different experience, and it would be able to teach me important biological skills and techniques needed for working in a laboratory setting as biomedical engineer.

At ABI, I performed many experiments under Dr. Banerjee’s tutelage to ascertain the effects of Vitamin D and Trichostatin A on human airway smooth muscle cells. To do this, I was taught how to carry out ELISA experiments to determine chemokine and cytokine expression levels in human airway smooth muscle cells after they were stimulated with Tumor Necrosis Factor and/or Interferon; this began with the proper aliquoting of laboratory solutions and ended with operating the ELISA plate reader and analyzing the resulting data. Throughout the ELISA process, I was able to hone my skills in pipetting and work at decreasing cross-contamination when moving from project to project. Not only did Dr. Banerjee help me with my technique and provide me with academic guidance, but I also learned a lot from my other colleagues in the laboratory. I learned how to perform Western Blots and I used this assay to determine protein expression levels. I was also introduced to cell culture and the procedure for maintaining the cell lines used in the laboratory. In addition to these techniques, I was able to aid in the prepping of human fatal asthmatic and normal lung tissue, and I was taught how to perform precision cut lung slice experiments, which I used to generate preliminary in vivo data for several projects.

Overall, my experience at ABI was very enlightening. Having never worked in a laboratory before, I enjoyed the family-like atmosphere between my colleagues and myself. ABI also helped me augment my presentation skills by having weekly lab meetings where I was responsible for presenting the data that I generated. At lab meeting, there were knowledgeable experts who were able to critique, question, and examine what I had done and provide me with helpful comments to point me in the right direction and the next step that I needed to take. With the experience and knowledge that I have gained, I can say ABI has had a positive impact on me and provided me with the proper tools for my future endeavors. I would recommend ABI to anyone, beginner or expert, looking to gain meaningful laboratory research experience.

 

Mi Thant Mon SoeMy co-op at the Airways Biology initiative (ABI) has been a great and invaluable experience. I was able to learn from a team of awesome researchers and be at the forefront of scientific innovation. One of the first things that I learned to do was performing Enzyme-linked immunosorbent assays (ELISA) to determine the amount of cytokines and chemokines released by the precision cut lung slices (PCLS) under the tutelage of Dr. Zhao and Dr. Koziol-White. I would then present the results at the weekly lab meetings where my results were evaluated and pieced together with the results from other ongoing experiments done by other researchers. Before my time at ABI, I had very little experience with cell culture and biological pathways and signaling. Under Dr. Cao’s guidance, I was able to have a hands-on experience with cell line maintenance, Western blots, and immunofluroscence staining. He would explain why I was doing every step of the procedure and how the procedure could be modified to optimize the usage of my time and resources. I love challenges and when I spoke to our lab manager about learning more techniques, I was trained to maintain transgenic mice colonies for Dr. Goncharova. This involved cross breeding different strains of mice and genotyping the pups to test for homozygosity and heterozygosity of the target genes.

Since there were other interns and students in the lab, in between different steps in my experiments, I had the opportunity to learn from them about their projects and the experiments they were working on. To make this amazing experience complete, everyone in the lab was very helpful and we had amazing social events every now and then. It is truly a place of sharing not just of knowledge but of ideas and culture, too. I had a wonderful experience working at ABI and I strongly recommend it to any student looking for a wonderful learning experience.

 

Stephanie KubalaAs a Mayock Fellowship recipient for the summer of 2013, I had chosen to work in Dr. Angela Haczku’s laboratory in order to expand my passion for allergy/immunology research by investigating ozone’s effects on asthma. I am very glad I chose to work in this lab this summer because of all the techniques I learned and all the people I was able to meet. I was able to foster collaborations between laboratories and perform independent projects utilizing patient nasal polyps and air liquid interface co-cultures.

Also, as a participant in the STEER program, I was able to simultaneously learn about the public and environmental health side of pollution through lectures and field trips. It culminated in an end of the summer symposium where I was able to present my summer research. This summer has been a mixture of fun and hard work that has increased my interest in conducting research concerning allergies and asthma. I hope to also present my work at the 2014 AAAAI Annual Meeting.

Summer of 2012

Megam milavecI have had a great experience working in the Airways Biology Initiative’s research program this summer. I came to ABI with limited lab experience, and everyone was very patient and helpful. I have learned so much over the past few months, which I am extremely thankful for. I have learned many different techniques and other information that I believe will be useful to me in the future.

The ABI lab has a very relaxed atmosphere, so not only was I learning a ton, but I was also enjoying myself while doing it. The staff is friendly and approachable, and they are all experts at what they do. My mentors were very knowledgeable and I had a great time working with them and learning from them. It was also nice to get to know some of the other students working in the lab and hearing about their projects. ABI is really a unique place to work because there are so many different projects in different areas of research going on at the same time. Overall, my summer experience with ABI has been very positive and beneficial, and I’m so glad I was given this opportunity!

 

Edwin 1.JPGDuring the summer before my second year of medical school, I was very fortunate to do an internship through the Airways Biology Initiative’s Summer Research Program.ABI is a world class airway biology lab with human asthma models that are difficult to find in other airway biology labs.Under Dr. Panettieri's mentorship, I was given the opportunity to measure intracellular calcium in airway smooth muscle using calcium fluorimetry.Additionally, I was able to practice presenting data during weekly lab meetings. I also learned a great deal about cutting edge asthma research during these lab meetings.

As a result of the mentorship and encouragement of the researchers at ABI, I've really come to love research and hope to pursue it as part of my career. I've also become friends with the awesome students who work at ABI. I would encourage anyone interested in biomedical research/medicine to participate in this internship.You can be sure that you will obtain valuable research experience and mentorship that would be difficult to find elsewhere.


Gloria 1During the summer of 2012, I was fortunate to participate in the Airways Biology Initiative’s Summer Research Program. I had the opportunity to work with several professionals while learning many basic science techniques. Under Dr. Hong and Mrs. Li’s guidance, I learned about Birt-Hogg-Dube syndrome, a genetic disease of which I had never known of before this summer. The research I participated in was focused on understanding the TFE3 transcription factor and more specifically the over-expression of lysosomal genes that occurs due to the starvation of HEK293 cells. By contributing to the research, I was able to gain valuable hands on experience culturing cells, performing Western blots, RT-PCRs, as well as, learn many other laboratory techniques and procedures. I also was given the opportunity to analyze and interpret the data I generated from the experiments I performed.

As a Penn student, I feel that I am extremely lucky to be able to attend various science and laboratory courses taught by experts in their fields. With the ABI summer program, the research and experimentation that I was able to perform allowed me to put into practice, what I learned in the classroom and it provided me with invaluable hands-on experiences. All of the ABI researchers were more than willing to answer all of my seemingly never-ending questions and there seemed to be endless opportunities to learn something new, during my summer internship. Overall my summer spent at ABI was enlightening and most definitely, rewarding.

 

Christina 1.JPGWhen I arrived to the Airway Biologics Initiative (ABI) lab at the University of Pennsylvania I received such a warm welcome that I knew my experience here was going to be great. On the start of my first day, I was immediately working with human lung tissue, making slices, harvesting tissue, and learning how each part of the lung can used in the laboratory. As a Drexel University student enrolled in the co-op program we are told that some of the best learning is by actually doing. It was clear that I was going to be doing a lot of learning.

One of my main focuses here at ABI was cell line maintenance and preparations for many of our collaborators. I also investigated pathways involved in many asthmatic and inflammatory responses within airway smooth muscle cells. Some of the laboratory techniques that I used during my time at ABI were: Precision Cut Lung Slices (PCLS), immunohistochemistry staining, gel electrophoresis, tissue culture, RNA and DNA isolations.

My favorite aspect of working at ABI was the environment. Every last person that is a part of ABI has been endlessly helpful and have given me such guidance. I never felt hesitant to ask a question or to propose an idea. Whatever I had to say, it was accepted and explored which makes science so exciting. The ABI team is very committed in teaching young students how to make a career out of science. The team members have a variety of degrees and have taken various different roads in their careers, which was useful in asking questions to help guide me into my path. My experience at ABI helped me build strong laboratory technical skills and well as learning where I fit in the science field.

 

Caroline ZhongThis summer at the ABI was my first formal experience in a scientific laboratory, and I learned a lot of basic and advanced techniques including cell culture, assaying cellular samples, and harvesting lung tissue samples for the study of asthma and other lung diseases. For cell culture, I learned how to prepare feeding and stock media for the cells to grow or “starve” in, I learned to plate and passage cells, to treat and stimulate cells, and finally how to lyse the cells for further analysis.

I also learned a lot of general lab techniques that included performing ELISAs, Western Blotting (immuno-blotting), BCA protein assay, cell-staining and cyto-spin, cell-counting, and of course, how to perform the data analysis in Microsoft Excel, all of which I had the opportunity to present at the weekly lab meetings.

In regards to harvesting and processing samples, I had the opportunity to observe my mentor Dr. Cynthia Koziol-White inflate a human lung with low-melting point agarose, core sections of the lung containing airways, and slice the cores using Krumdieck tissue slicer to turn them into Precision Cut Lung Slices (PCLS). These lung slices were then treated with different drugs which would cause the airways to either contract or dilate in response to drugs like Carbachol, Isoproterenol, Formoterol, Salmeterol, Albuterol, Forskolin, Dexamethasone, Budesonide, and other beta adrenergic receptor agonists and steroids, which I was already familiar with thanks to the scientific papers also provided to me by my mentor. It wasn’t just about watching my mentor perform these techniques. I also had hands on opportunity to harvest and process mouse lung samples, I got to inflate the left lung through a catheter in the trachea, and perform a retro-orbital eye bleeds to collect blood samples for future analysis.

The summer flew by before I knew it, but it was full of learning and I made a lot of friends. I would definitely recommend the ABI’s summer program to anyone interested in getting some lab experience; its fun and rewarding and a really awesome way to spend the summer.

 

Kevin 3.JPGI began working at ABI in April 2012 for my third and final co-op as a Drexel student. I had previous lab experience and felt very comfortable working in a laboratory setting. Like any new job, however, my work at ABI was a little intimidating at first, but everyone was dedicated to helping and teaching each new employee, making the transition much smoother. We received a human lung on my first day and I began working immediately, coring and slicing airways in the lung tissue. The best part about my experience at ABI has been the fact that you gain your independence very quickly, but you are never alone. Whenever I needed help or clarification for an experiment, there was never a shortage of knowledge and experience within the lab to turn to.  I could always turn to Dr. Cao when running western blots, Dr. Koziol-White with any questions about PCLS experiments, Dr. Zhou for help with ELISAs, and of course my mentor, Dr. Banerjee, for learning new techniques, plating and stimulating cell lines, learning about his interest in vitamin D and HDAC inhibition, and guidance in my decision to follow a career in medicine. Dr. Panettieri is one of the most knowledgeable and respected doctors I have ever had the privilege of meeting, and he is never too busy to stop and explain, in detail, every receptor, cascade, and pathway involved in asthma symptoms and treatment.

Many people may think an internship is all work and no play, or vice versa, but at ABI I think there is a good mix of both. There’s no doubt that they expect a lot of work to get done, and done correctly, but events such as the ABI annual picnic and the business persons’ special at the Phillies game are a great escape from the busy work day. It was at these events that I really had the opportunity to get to know my co-workers and supervisors on a personal level. Overall, my time at ABI has been one of the most interesting, knowledge-building, and interactive research opportunity I have had the privilege of experiencing.

 

Summer of 2011

Jackie ScalaThis past summer I had the opportunity to work as an intern at Airways Biology Initiative’s Summer Research Program. ABI provided me with a valuable research experience that would be very difficult to find elsewhere. I was supervised by Dr. Koziol-White, who showed me what projects were going on in the lab and taught me many laboratory techniques. In addition to observing human lung and trachea dissections, I was able to perform ELISA assays, complete dose response curves with human lung slices, maintain human airway smooth muscle cell cultures, present data at weekly lab meetings, and be a part of an excellent research team. I now feel very confident in my ability to complete the laboratory techniques that I learned this summer because of the way that ABI trained me. First, I was shown a laboratory procedure. Then, I was observed while completing that same procedure. Finally after that, I was able to complete that procedure independently. By teaching in this manner, it was extremely valuable and I can say with confidence that I know exactly how to do an ELISA or how to maintain cells in culture. This approach to teaching the various laboratory protocols procedures was very beneficial and significantly helped me to increase my laboratory skills.

This summer research program also helped me decide what type of career I would like to pursue in the future. By asking PhDs and MDs at ABI about their graduate school or medical school experience, I gained helpful career advice. I would definitely recommend this internship for anyone who is interested in a career in science or medicine. Not all research programs for undergraduate students will offer the opportunity to work with human tissue and learn so many laboratory techniques. I learned a lot in just one summer at ABI and this program was definitely a worthwhile experience.

 

Martin JohnsonI had the privilege of working for the University of Pennsylvania’s Airways Biology Initiative (ABI) as a laboratory research intern under the guidance of Dr. Cao & Dr. Panettieri. This was far from the normal monotonous undergraduate job of staring at a computer or accomplishing mindless clerical work. In my position, I was held to the same standards and expectations as the other hardworking ABI laboratory staff. Although it was a little overwhelming at first, the experience was well worth it since I learned a quite bit about medicine, medical occupations, laboratory techniques, and the professional work environment. As a result, I was constantly busy with complicated molecular biology experimental work and I was given the opportunity to learn and practice many cutting edge experimental techniques.

My specific responsibilities included assisting in the maintenance and propagation of ABI’s human airway smooth muscle cells. These cells are essential for the experiments and different collaborations of the ABI labs. In maintaining and propagating these cells, I was afforded the opportunity to learn many different techniques such as, dissecting human lung tissue, trypsinizing cells, freezing cells, stringent aseptic methods, and several different immunohistochemistry techniques which were necessary to characterize these cells. In addition, to those responsibilities I also had the opportunity to perform experiments under Dr. Cao’s supervision, to investigate the protein signaling pathways used by contractile agents to elicit contraction in human airway smooth muscle cells. It was extremely rewarding for me to independently (of course guidance was given often) design, perform, and present the results of these experiments. I found it to be very gratifying and exciting to be a part of the innovation and experimentation of such groundbreaking research. Dr. Cao’s research also required me to learn several other techniques such as western blotting, RNA isolation, Polymerase Chain Reaction (PCR), and Enzyme-Linked Immunosorbent Assay (ELISA).

Overall, this was an extremely positive experience for me and I strongly recommend any motivated and interested student to apply for this fantastic opportunity to work at the ABI labs. The experience to work with a strong professional team and great people, as well as, learn life lessons and have fun is well worth it!

 

Summer of 2010

Description: Michael Phan 2.jpgI spent my first co-op at the Airways Biology Initiative (ABI). I really enjoyed working there, as this was my first job-related work experience in a professional environment. Working at ABI was unlike any of the other typical internships/co-ops that I had heard about. There wasn't any filing papers or weeks spent at the computer endlessly surfing the web, counting the minutes until the end of the day. As a student at ABI, I was kept very busy and felt the pressure to perform and to be productive. Every individual there has a wealth of knowledge which can be somewhat intimidating, yet at the same time they were all extremely friendly and helpful, and they were always willing to answer any of my questions or help me whenever I needed it.

During my co-op at ABI much of my time was spent performing cell biology and molecular biology experiments. One of my main responsibilities was to maintain and culture cell lines developed in the lab. I had the opportunity to routinely isolate human airway smooth muscle cells, fibroblast and macrophages from human lung tissue. Another of my responsibilities included measuring mediators released through an Enzyme-Linked Immunosorbent Assays or ELISAs. In addition to these responsibilities I had the opportunity to learn or observe several other techniques and procedures performed in the laboratory. They included Precision Cut Lung Slices (PCLS), murine FlexiVent (mouse lung function testing), immunohistochemistry staining, and immunocytochemistry staining.

Overall my experience at ABI has been extremely positive and it has given me a strong sense of the demands required to work in a wet bench laboratory. I would strongly recommend the Airways Biology Initiative laboratory to any student who is interested in a career in science and who is looking to gain practical experience in translational research.