Margaret M. Chou, Ph.D.
Margaret M. Chou, Ph.D.
Associate Professor of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine
Graduate Group Affiliations
Contact information
3615 Civic Center Boulevard
ARC Bldg, 816E
Philadelphia, PA 19104-6058
ARC Bldg, 816E
Philadelphia, PA 19104-6058
Office: 267-426-9228
Fax: 267-426-5165
Fax: 267-426-5165
Email:
mmc@mail.med.upenn.edu
mmc@mail.med.upenn.edu
Publications
Links
Search PubMed for articles
Pharmacological Sciences graduate group faculty webpage.
Cell and Molecular Biology graduate group faculty webpage.
Search PubMed for articles
Pharmacological Sciences graduate group faculty webpage.
Cell and Molecular Biology graduate group faculty webpage.
Education:
B.A. (Biochemistry)
Barnard College (NY), 1987.
Res. Asst (Biochemistry/Cell Biology)
Rockefeller University (NY), 1989.
Ph.D. (Molecular Oncology)
Rockefeller University, 1993.
Post-doc (Cell Biology)
Harvard Medical School (MA), 1997.
Permanent linkB.A. (Biochemistry)
Barnard College (NY), 1987.
Res. Asst (Biochemistry/Cell Biology)
Rockefeller University (NY), 1989.
Ph.D. (Molecular Oncology)
Rockefeller University, 1993.
Post-doc (Cell Biology)
Harvard Medical School (MA), 1997.
Description of Research Expertise
Research Interests- mechanisms of malignant transformation
- functions/regulation of small GTPases during transformation
- ubiquitination
- NFkB
Key words: G proteins, transformation, ubiquitination.
Description of Research
The transformation of a normal cell to a cancerous one is a complex process that entails the accumulation of multiple genetic alterations. These mutations impact multiple cellular functions, including the proliferative, survival, and migratory/metastatic properties of the cell. Our global interests focus on how GTPases of the Rho family deregulate these processes during malignant progression. Toward this end, we recently identified the TRE17/USP6 oncogene as a novel effector of Rho GTPases.
TRE17 was recently identified as a target of chromosomal translocation in an aggressive pediatric bone tumor known as aneurysmal bone cyst (ABC). Over 60% of ABCs harbor translocation of the TRE17 locus, leading to it transcriptional unregulation. However, virtually nothing is known about the mechanism by which TRE17 overexpression leads to the formation of these highly complex, cellularly heterogeneous lesions. The goal of our work is to identify the cellular pathways through which TRE17 functions, and to develop murine models of ABC.
Lab personnel:
Lashon Pringle (Graduate Student)
Laura Quick (Research Specialist)
Ying Ye (Post-doctoral associate)
Rob Young (Research Specialist)
Selected Publications
Ye Y, Pringle LM, Lau AW, Riquelme DN, Wang H, Jiang T, Lev D, Welman A, Blobel GA, Oliveira AM, Chou MM.: TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB. Oncogene In press, April 2010.Blobel, G.A., Kadauke, S., Wang, E., Lau, A.W., Zuber, J., Chou, M.M., and Vakoc, C.R.: A reconfigured pattern of MLL occupancy within mitotic chromatin promotes rapid transcriptional reactivation following mitotic exit. Molecular Cell 36(6): 970-983, December 2009.
Fellig, Y., Oliveira, A.M., Margolin, E., Gomori, J.M., Erickson-Johnson, M.R., Chou, M.M., Umansky, F., and Soffer, D.: Extraosseous aneurysmal bone cyst of cerebello-pontine angle with USP6 rearrangement. Acta Neuropathology 118: 579-581, Sept 2009.
Holloway BA, Gomez de la Torre Canny S, Ye Y, Slusarski DC, Freisinger CM, Dosch R, Chou MM, Wagner DS, Mullins MC.: A novel role for MAPKAPK2 in morphogenesis during zebrafish development. PLoS Genetics 5(3): e1000413, March 2009.
Lau, AW and Chou, MM.: The adaptor complex AP-2 regulates post-endocytic trafficking through the non-clathrin Arf6-dependent endocytic pathway. Journal of Cell Science 121(24): 4008-4017, Nov 2008.
Sukov WR. Franco MF. Erickson-Johnson M. Chou MM. Unni KK. Wenger DE. Wang X. Oliveira AM.: Frequency of USP6 rearrangements in myositis ossificans, brown tumor, and cherubism: molecular cytogenetic evidence that a subset of "myositis ossificans-like lesions" are the early phases in the formation of soft-tissue aneurysmal bone cyst. Skeletal Radiology 37(4): 321-7, Apr 2008.
Liu, Jay. Lamb, Darija. Chou, Margaret M. Liu, Yong-Jian. Li, Guangpu.: Nerve growth factor-mediated neurite outgrowth via regulation of Rab5. Molecular Biology of the Cell 18(4): 1375-84, Apr 2007.
Robertson SE., Setty SR., Sitaram A., Marks MS., Lewis RE., Chou MM.: Extracellular signal-regulated kinase regulates clathrin-independent endosomal trafficking. Molecular Biology of the Cell 17(2): 645-57, Feb 2006.
Shen, Chuanlu. Ye, Ying. Robertson, Sarah E. Lau, Alan W. Mak, Don-On D. Chou, Margaret M.: Calcium/calmodulin regulates ubiquitination of the ubiquitin-specific protease TRE17/USP6. Journal of Biological Chemistry 280(43): 35967-73, Oct 28 2005.
Martinu, Lenka. Masuda-Robens, Jeffrey M. Robertson, Sarah E. Santy, Lorraine C. Casanova, James E. Chou, Margaret M.: The TBC (Tre-2/Bub2/Cdc16) domain protein TRE17 regulates plasma membrane-endosomal trafficking through activation of Arf6. Molecular & Cellular Biology 24(22): 9752-62, Nov 2004.