Hao Shen, Ph.D.
Associate Professor of Microbiology
Department: Microbiology
Graduate Group Affiliations
Contact information
303D Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104
3610 Hamilton Walk
Philadelphia, PA 19104
Office: (215) 573-5259
Fax: (215) 573-9068
Fax: (215) 573-9068
Email:
hshen@mail.med.upenn.edu
hshen@mail.med.upenn.edu
Publications
Links
Search PubMed for articles
Primary Work Website
Immunology graduate group faculty webpage.
Cell and Molecular Biology graduate group faculty webpage.
Parasitology graduate group faculty webpage.
Search PubMed for articles
Primary Work Website
Immunology graduate group faculty webpage.
Cell and Molecular Biology graduate group faculty webpage.
Parasitology graduate group faculty webpage.
Education
B.S.
Jiangxi University, China, 1983.
Ph.D.
University of California at Riverside, 1992.
B.S.
Jiangxi University, China, 1983.
Ph.D.
University of California at Riverside, 1992.
Post-Graduate Training
Postdoctoral Fellow Dr. Jeff Miller/Dr. Rafi Ahmed, UCLA School of Medicine, 1993-1997.
Permanent linkPostdoctoral Fellow Dr. Jeff Miller/Dr. Rafi Ahmed, UCLA School of Medicine, 1993-1997.
Description of Research Expertise
Research InterestsImmune surveillance of intracelluar and emerging infectious agents.
Key words: Listeria, Ebola, T lymphocyte, bacterial pathogenesis, vaccine.
Description of Research
We are interested in understanding how the microbial virulence strategies affect the nature and magnitude of the host response and how the resulting immune response influences the course of infection. We use the intracellular bacterium Listeria monocytogenes, a category B agent, as a model and employ a multidisciplinary approach that combines recent technical and conceptual advances in molecular genetics, microbial pathogenesis, cell biology and immunology. By genetically manipulation of the bacterium, we are identifying different aspects of bacterial antigens that influence their ability to induce a T cell response and to serve as a protective target. We are also combining our ability to genetically manipulate the bacterium with the use of knock-out mice to examine how various immune effectors counter different microbial virulence factors and to identify new immune correlates of protection.
We are also applying the knowledge gained from our basic research and our expertise in both microbial pathogenesis and cellular immunology to study emerging pathogens such as Ebola virus and B. anthracis. We are identifying T cell epitopes in Ebola and examining how the soluble glycoprotein (sGP) of Ebola may interfere with the ability of dendritic cells to prime T cell responses. We are investigating the role of phospholipases and hemolysin in mediating escape of B. anthracis from phagosome of macrophages and are studying the early immune response to the pulmonary form of anthrax infection using various KO murine models. The long-term objective of our studies is to provide a platform for rational design of effective vaccines to combat today’s complex and evolving diseases.
Rotation Projects for 2006-2007
1. Modulation of immune response by bacterial virulence factors
2. Molecular basis of functional T cell memory
Lab personnel:
Alison Crawford, PhD
Connie Krawczyk, PhD
Luana Atherly, PhD
Joanna DiSpirito
Erika Pearce
John Northrop
Amy Troy
Selected Publications
Listeria monocytogenes: pathogenesis and host resopnse. H. Goldfine and H. Shen (eds.). Springer Publisher, 2005.Zenewicz LA, Skinner JA, Goldfine H, Shen H : Listeria monocytogenes Virulence Proteins Induce Surface Expression of Fas Ligand on T lymphocytes. Molecular Microbiology 51(5): 1483-1492, March 2004.
Chua MM, Macnamara K, San Mateo LR, Shen H, Weiss SR: Effects of an epitope specific CD8+ T cell response on murine coronavirus CNS disease: protection from virus replication and antigen spread and selection of epitope escape mutants. Journal of Virology 78(3): 1150-1159, February 2004.
Nichols KE, Haines K, Myung PS, Newbrough S, Myers E, Jumaa H, Shedlock DJ, Shen H, Ahmed J, Ali H, Koretzky GA: Macrophage activation and Fc-receptor-mediated signaling do not require expression of the SLP-76 and SLP-65 adaptors. J. Leuk Biol. 75(3): 541-552, March 2004.
Simmons G, Lee A, Rennekamp AJ, Fan X, Bates P, Shen H: Identification of murine T cell epitopes in Ebola virus nucleoprotein. Virology 318(1): 224-230, January 2004.
Starks H, Bruhn KW, Shen H, Barry RA, Dubensky TW, Brockstedt D, Hinrichs DJ, Higgin DE, Miller JF, Giedlin M, Bouwer HGA: Listeria monocytogenes as a vaccine vector: virulence attenuation or existing anti-vector immunity does not diminish therapeutic efficacy. Journal Immunology 173(1): 420-427, July 2004.
Skinner, J., Reissinger A, Shen H, Yuk M: Bordetella type III secretion and adenylate cyclase toxin synergize to drive dendritic cells into a semi-mature state. Journal of Immunology 173(3): 1934-1940, August 2004.
Pearce El, Shedlock DJ, Shen H: Functional characterization of MHC Class II-Restricted CD8+CD4- and CD8-CD4- T Cell Responses to Infection in CD4-/- Mice. Journal of Immunology 173(4): 2494-2499, August 2004.
Zheng S, Jiang J, Shen H and Chen Y: Reduced apoptosis and listeriosis in TRIAL-null mice. Journal of Immunology 173(9): 5652-5658, November 2004.
Northrop JC, Shen H: CD8+ T-cell memory: only the good ones last. Curr Opin Immunol 16(4): 451-455, August 2004.
