Douglas A. Coulter, PhD

faculty photo
Professor of Pediatrics
Department: Pediatrics
Graduate Group Affiliations

Contact information
Room 410D
Abramson Pediatric Research Building
3516 Civic Center Blvd.
Philadelphia, PA 19104-4318
Office: (215) 590-1937
Fax: (215) 590-4121
Education:
B.S. (Biology with honors)
Trinity College, Hartford, CT, 1980.
M.S. (Zoology)
University of Rhode Island, 1983.
Ph.D. (Biology)
Boston University Marine Program, Marine Biological Laboratory, Woods Hole, MA, 1986.
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Description of Research Expertise

KEY WORDS:
epilepsy, hippocampus, GABA receptors, thalamus

RESEARCH INTERESTS
Epilepsy, neuronal excitability, CNS rhythm generation, GABA receptors, development of neurotransmitter receptors and ion channels, synaptic function

RESEARCH TECHNIQUES
Patch clamp recordings, extracellular and intracellular recordings, immunohistochemistry, molecular biology (including aRNA amplification mRNA expression profiling in individual neurons), neuronal cell culture, transgenic animals, in vitro and in vivo recording techniques, optical recordings, EEG recordings.

RESEARCH SUMMARY
My research interests center on understanding the cellular and molecular mechanisms underlying the development of epilepsy. Symptomatic seizure disorders such as temporal lobe epilepsy are among the most prevalent and least medically responsive forms of epilepsy. They are also among the most interesting. A presumably normal individual receives some injurious stimuli, which, at some distant time point results in the initiation of an epileptic state, characterized by recurrent spontaneous seizures. A better understanding these seizure-initiating mechanisms should facilitate development of enhanced therapeutic strategies to improve treatment, and perhaps eventually contribute to the development of a cure for epilepsy.

My laboratory uses physiological, anatomical, and molecular techniques to address experimental issues relevant to epilepsy. Physiologically, my colleagues and I use patch clamp, intracellular, and extracellular recording techniques in both in vitro and in vivo preparations of animal or human brain. Anatomically, we use immunohistochemical and conventional staining techniques to characterize alterations occurring in the epileptic brain at a circuit level, including loss of populations of neurons, alterations in expression patterns of proteins, and axonal remodeling. Molecularly, we use a combination of semi-quantitative profiling of mRNA expression levels at the single cell level, in situ hybridization, retroviral transfection techniques, and antisense oligonucleotide knockdown of expression of certain proteins. The combination of these three diverse experimental approaches provides a powerful, synergistic approach to better understand critical factors contributing to the initiation of the epileptic condition.

Selected Publications

Cohen, A.S., Lin, D.D., Quirk, G.L. and Coulter, D.A.: Dentate granule cell GABAA receptors in epileptic hippocampus: enhanced synaptic efficacy and altered pharmacology. European Journal of Neuroscience 17: 1607-1616, 2003.

Burton, D.Y., Cunxian, S., Fishbein, I., Hazelwood, S., Li, Q., Defelice, S., Connolly, J.M., Perlstein, I., Coulter, D.A., and Levy, R.J.: The incorporation of an Ion channel gene mutation associated with the Long QT syndrome (Q9E-hMiRPI) in a plasmid vector for site-specific arrhythmia gene therapy: In Vitro and In Vivo feasibility studies. Human Gene Therapy 14: 907-922, 2003.

Rafiq, A., Gong, Q.Z., Lyeth, B.G., DeLorenzo, R.J., and Coulter, D.A.: Induction of prolonged electrographic seizures in vitro has a defined threshold and is all or none: implications for diagnosis of status epilepticus. Epilepsia 44(8): 1034-1041, 2003.

Rikhter, T.Y., Hsu, F.C., and Coulter, D.A.: Gene expression alterations in CA1 area of the rat hippocampus before the onset of epilepsy: A microchip study. American Epilepsy Society submitted 2003.

Ang, C.W., Carlson, G.C., and Coulter, D.A.: Stratum oriens/Alveus interneurons spatially segregate and gate excitatory inputs onto CA1 neurons: voltage sensitive dye imaging study. Society for Neuroscience Abstracts submitted 2003.

Pathak, H.R., Kelly, M.E., Ackman, J., LoTurco, J.J., and Coulter, D.A.: Synaptic integration of newborn neurons in adult dentate gyrus following status epilepticus. Society for Neuroscience Abstracts submitted 2003.

Crino, P., Jin, H., Rikhter, T.Y, Robinson, M.B., Coulter, D.A., and Brooks Kayal, A.R.: Increased expression of the neuronal glutamate transporter (EAAT3/EAAC1) in hippocampal and neocortical epilepsy. Epilepsia 43(3): 211-218, 2002.

Gibbs III, J.W., Zhang, Y.-F., Ahmed, H.S., and Coulter, D.A.: Anticonvulsant actions of Lamotrigine on spontaneous thalamocortical rhythms. Epilepsia 43(4): 342-349, 2002.

Itoh, T., Beesley, J., Itoh, A., Kavanaugh, B., Cohen, A.S., Coulter, D.A., Grinspan, J.B., and Pleasure, D.: AMPA glutamate receptor-mediated calcium signaling is transiently enhanced during development of oligodendrocytes. Journal of Neurochemistry 81: 390-402, 2002.

Coulter, D.A., McIntyre, D.C., and Loscher, W.: Animal models of limbic epilepsies: What can they tell us? Brain Pathology 12: 240-256, 2002.

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Last updated: 08/02/2013
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