Mark Haskins VMD

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Graduate Group Affiliations

Contact information
4020 Ryan/Vet
3900 Delancey St
Philadelphia, PA 19104-6051
Office: (215) 898-4852
Fax: (215) 898-0719
Education:
BA (Pre-Vet)
Pennsylvania State University, 1966.
VMD (Veterinary Medicine)
University of Pennsylvania, 1969.
MS (Biomedical Engineering)
Drexel University, 1974.
Ph.D (Pathology)
University of Pennsylvania, 1979.
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Description of Research Expertise

Research Interests
Discovery, characterization, and therapy of large animal models of human genetic disease.

Key words: Dog, cat, lysosomal storage disease.

Description of Research
In collaboration with others, a series of metabolic diseases caused by deficient enzyme activities are being studied, including mucopolysaccharidoses (MPS) I, VI, and VII (alpha-L-iduronidase, 4-sulfatase, and ß-glucuronidase deficiencies, respectively), alpha mannosidosis (alpha-mannosidase deficiency), I-cell disease (N-acetylglucosamine-1-phosphotransferase), Krabbe disease (galactosylceramidase), and acute intermittent porphyria (PBG-deaminase deficiency). Five of these diseases are lysosomal storage disorders with clinical phenotypes in the animals that are the same as in affected children. Experiments include administering recombinant human alpha-L-iduronidase intravenously to cats with MPS I and gene therapy using retroviral and AAV vectors. The gene therapy experiments are being performed in MPS I cats and dogs, MPS VI cats, and MPS VII dogs using the species-specific cDNAs except feline MPS I. The somatic cell gene therapy experiments include a) intraocular injections of AAV-4-sulfatase vector virus in MPS VI cats, b) neonatal intravenous retrovirus gene therapy in MPS I and VI cats, MPS I dogs, and MPS VII dogs, and c) intramuscular AAV vectors containing the feline 4S cDNA and human SUMF-1 cDNA.

Rotation Projects
Please see Dr. Haskins.

Lab personnel:
Thomas O'Malley, Lab Research Specialist
Ping Wang, Lab Reseach Specialist
Ulana Prociuk, Lab Research Specialist
Angie Huff , Lab Reseach Specialist
Patty O'Donnell, Animal Research Specialist
Karyn Cullen, Animal Technician


PRIMARY AREAS OF RESEARCH COMPETENCE

Pathology
Genetics

Selected Publications

Ellinwood, N.M., Vite, C.H., and Haskins, M.E. : Gene therapy for lysosomal storage diseases: The lessons and promise of animal models. J. Gene Medicine 6: 481-506, 2004.

Mango, RL, Xu L, Sands, MS, Vogler, C, Seiler, G, Schwarz, T, Haskins, ME, Ponder, KP: Neonatal retroviral vector-mediated hepatic gene therapy reduces bone, joint, and cartilage disease in mucopolysaccharidosis VII mice and dogs. Mol Genet Metab 82: 4-19, 2004.

Sleeper, M.M., Fornasari, B., Ellinwood, N.M., Weil, M.A., Melniczek, J., O’Malley, T.M., Sammaroc, C.D., Xu, L., Ponder, K.P., and Haskins, M.E. : Gene therapy ameliorates cardiovascular disease in dogs with mucopolysaccharidosis VII. Circulation 2004 Notes: in press.

Suter, S.E., Gouthro, T.A., McSweeney, P.A., Nash, R.A., Haskins, M.E., Felsburg, P.J., Henthorn, P.S.: Isolation and characterization of pediatric canine bone marrow CD34+ cells. Vet. Immunol. and Immunopathology 2004 Notes: in press.

Xu, L., O’Malley, T., Sands, M.S., Wang, B., Meyerrose, T., Haskins, M., Ponder, K.P. : In vivo transduction of hematopoietic stem cells after neonatal intravenous injection of an amphotropic retroviral vector in mice. Molecular Therapy 2004 Notes: in press.

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Last updated: 08/26/2013
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