Hua-Ying Fan, Ph.D.

faculty photo
Assistant Professor of Biochemistry and Biophysics
Department: Biochemistry and Biophysics

Contact information
Epigenetics Program
Department of Biochemistry and Biophysics
Perelman School of Medicine
9-133 Smilow Center for Translational Research
3400 Civic Center Blvd
Philadelphia, PA 19104-6059
Office: 215 573-5705
Lab: 215 573-5713
Education:
B.S. (Chemistry)
National Tsing-Hua University, Hsin-Chu, Taiwan, 1989.
M.S. (Chemistry)
New York University, New York, NY, 1990.
Ph.D. (Cellular and Molecular Biology)
Sackler Institute of New York University Medical Center, New York University, New York, NY, 1996.
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Description of Research Expertise

The Fan lab is interested in understanding the mechanisms of epigenetic regulation. Epigenetics describes the process by which a specific transcriptional program, induced by a signal, is maintained and inherited through cell divisions without the necessary presence of the original signal. Chromatin structure holds the secrets of epigenetic regulation. To dissect the mechanisms of epigenetic regulation, our research is focused in two main directions. First, we study how ATP-dependent chromatin remodelers regulate chromatin structure and how defects in these activities can lead to disease. Currently, we are using CSB (Cockayne Syndrome complementation group B) as a model protein to understand the function of ATP-dependent chromatin remodeling in DNA repair, aging and cancer. Second, we wish to understand how epigenetic regulatory mechanisms influence cancer cell self-renewal and differentiation, mechanisms that might impact the programming and reprogramming capacity of cancer cells. Results from our studies will, therefore, not only shed light on fundamental mechanism of epigenetic regulation, but will provide novel insights into the causes and mechanisms of disease.


Current Members:

Erica Boetefuer, Graduate Student, Biology Graduate Program;
Robert Lake, Ph.D. Senior Research Investigator;
Mariel Coradin, Rotation Student, BMB Graduate Program

Selected Publications

Robert J. Lake, Erica L. Boetefuer, Pei-Fang Tsai, Jieun Jeong, Inchan Choi, Kyoung-Jae Won and Hua-Ying Fan: The Sequence-Specific Transcription Factor c-Jun Targets Cockayne Syndrome Protein B to Regulate Transcription and Chromatin Structure. PLoS Genetics 10(4): e1004284, April 2014 Notes: doi:10.1371/journal.pgen.100428.

Robert J. Lake, Pei-Fang Tsai, Inchan Choi, Kyoung-Jae Won and Hua-Ying Fan: RBPJ, the Major Transcriptional Effector of Notch Signaling, Remains Associated with Chromatin Throughout Mitosis, Suggesting a Role in Mitotic Bookmarking. PLoS Genetics 10(3): e1004204, March 2014.

Iltaeg Cho, Pei-Fang Tsai, Robert J. Lake, Asjad Basheer, and Hua-Ying Fan: ATP-Dependent Chromatin Remodeling by Cockayne Syndrome Protein B and NAP1-like histone chaperones is required for efficient transcription-coupled DNA repair. PLOS GENETICS 9(4): e1003407, April 2013.

Robert J. Lake and Hua-Ying Fan: Structure, Function and Regulation of CSB: A Multi-Talented Gymnast. Mechanisms of Ageing and Development. 134(5-6): 202-11, May-Jun 2013 Notes: Epub 2013 Feb 16.

Lake, R.J., Basheer, A., and Fan, H.-Y.: Reciprocally regulated chromatin association of the Cockayne syndrome protein B and p53. J. Biol. Chem. 286(40): 34951-8, Oct 7 2011 Notes: Epub 2011 Aug 18.

Lake RJ, Geyko A., Hemashettar G., Zhao Y. and Fan, H.-Y. : UV-induced association of the CSB remodeling protein with chromatin requires ATP-dependent relief of N-terminal autorepression. Molecular Cell 37: 235-246, 2010.

Lavigne M, Eskeland R, Azebi S, Saint-André V., Jang, S. M., Batsché,E., Fan, H.-Y., Kingston, R. E., Imhof, A., and Muchardt, C. : Interaction of HP1 and Brg1/Brm with the globular domain of histone H3 required for HP1-mediated repression. PLoS Genetics 5: e1000769. doi:10.1371/journal.pgen.1000769, 2009.

Kelly DF, Lake RJ, Middelkoop TC, Fan H-Y, Artavanis-Tsakonas S, and Waltz, T. : Molecular structure and dimeric organization of the Notch extracellular domain as revealed by electron microscopy. PLoS ONE 5: e10532. doi:10.1371/journal.pone.0010532 2010.

Trotter, K.W., Fan, H.-Y., Ivey, M., Kingston, R.E., Archer, T.K. : The HSA domain of BRG1 mediates critical interactions required for GR2 dependent transcriptional activation in vivo. Mol. Cell Biol. 28: 1413-1426, 2008.

Yu, A., Fan, H. -Y., Liao, D., Bailey, A. D. and Weiner, A. M. : Activation of p53 or loss of the Cockayne syndrome group B repair protein causes metaphase fragility of human U1, U2 and 5S genes. Molecular Cell 5: 801-810, 2000.

Fan, H.-Y., He, X., Kingston, R. E. and Narlikar, G. J. : Distinct strategies to make nucleosomal DNA accessible. Molecular Cell 11: 1311-1322, 2003.

Fan, H.-Y., Trotter, K., Archer, T., and Kingston, R. E. : Swapping function of two chromatin remodeling complexes. Molecular Cell 17: 805-815, 2005.

Dennis, J.H.*, Fan, H.-Y.*, Reynolds, M.S.*, Yuan, G., Meldrim, J, Richter, D.J., Peterson, D.G., Rando, O.J., Noble, W.S., Kingston R.E. (*These authors contribute equally to this work.): Independent and complementary methods for large-scale structural analysis of mammalian chromatin. Genome Res. 17: 928-999, 2007.

Zhang, Z., Fan, H.-Y., Goldman, J. A., and Kingston, R. E. : Homology driven chromatin remodeling by human Rad54. Nature Struct. Mol. Biol. 14: 397-405, 2007.

He, X., Fan, H.-Y., Garlick, J.D., and Kingston, R.E. : Diverse regulation of SNF2h chromatin remodeling by noncatalytic subunits. Biochemistry 47: 7025-7033, 2008.

Newman, J.C., Bailey, A.D., Fan, H.-Y., Pavelitz, T., and Weiner, A.M. : An abundant evolutionarily conserved CSB-PiggyBac fusion protein expressed in Cockayne syndrome. PLoS Genetics 4: e1000031, 2008.

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Last updated: 09/22/2014
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