Gerd A. Blobel, MD, PhD

faculty photo
Professor of Pediatrics
Department: Pediatrics
Graduate Group Affiliations

Contact information
316H Abramson Research Center
3615 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215) 590-3988
Fax: (215) 590-4834
Lab: (215) 590-4461
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Description of Research Expertise

Research Interests
higher order chromatin structure, gene expression, epigenetic memory, transcription factors, mitosis, BET proteins, gene therapy

Description of Research
Our lab focuses on the genetic and epigenetic control of hematopoiesis and its disorders. Specifically, we study how tissue-specific transcription factors govern the specification and maintenance of hematopoietic cell lineages. We examine how transcription programs are epigenetically transmitted through mitosis to maintain lineage identity. Our work is leading us deeply into the analysis of chromatin structure, its modifications and organization. For example, we investigate how regulatory elements are spatially organized in the nucleus. We are developing approaches to manipulate higher order chromatin organization as a therapeutic tool for the treatment of diseases affecting the globin genes. For our studies we combine molecular, genomic, biochemical, and imaging approaches with studies in normal and gene targeted mice.

Lab Rotation Projects:
For information about rotation projects please contact me directly.

Lab personnel:

Caroline Bartman, graduate student
Vivek Behera, combined degree student
Perry Evans, bioinformatician
Jeremy Grevet, combined degree student
Peng Huang, postdoctoral fellow
Eugene Khandros, postdoctoral fellow
Xianjiang Lan, postdoctoral fellow
Kiwon Lee, postdoctoral fellow
Jing Luan, combined degree student
Scott Peslak, postdoctoral fellow
Malini Sharma, technician
Marit Vermunt, postdoctoral fellow
Aoi Wakabayashi, graduate student
Hongxin Wang, technician/lab manager
Michael Werner, combined degree student
Jennifer Yano, technician
Adam Zhang, postdoctoral fellow
Di Zhang, combined degree student

Selected Publications

Grevet J.D., Lan X., Hamagami N., Edwards, C.R., Sankaranarayanan L., Ji X., Bhardwaj S.K., Face C.J., Posocco D.F., Abdulmalik O., Keller C.A., Giardine B., Sidoli S., Garcia B.A., Chou S.T., Liebhaber S.A., Hardison R.C., Shi J., and Blobel G.A.: Domain-focused CRISPR-screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells. Science in press 2018.

Behera V, Evans P, Face CJ, Hamagami N, Sankaranarayanan L, Keller CA, Giardine B, Tan K, Hardison RC, Shi J, Blobel GA: Exploiting genetic variation to uncover rules of transcription factor binding and chromatin accessibility. Nature Communications 2018.

Huang P, Keller CA, Giardine B, Grevet JD, Davies JOJ, Hughes JR, Kurita R, Nakamura Y, Hardison RC, Blobel GA.: Comparative analysis of three-dimensional chromosomal architecture identifies a novel fetal hemoglobin regulatory element. Genes and Development 2017.

Hsu SC, Gilgenast TG, Bartman CR, Edwards CR, Stonestrom AJ, Huang P, Emerson DJ, Evans P, Werner MT, Keller CA, Giardine B, Hardison RC, Raj A, Phillips-Cremins JE, Blobel GA: The BET Protein BRD2 Cooperates with CTCF to Enforce Transcriptional and Architectural Boundaries. Molecular Cell 2017.

Hsiung CC, Bartman CR, Huang P, Ginart P, Stonestrom AJ, Keller CA, Face C, Jahn KS, Evans P, Sankaranarayanan L, Giardine B, Hardison RC, Raj A, Blobel GA.: A hyperactive transcriptional state marks genome reactivation at the mitosis-G1 transition. Genes and Development 2016.

Bartman CR, Hsu SC, Hsiung CC, Raj A, Blobel GA: Enhancer Regulation of Transcriptional Bursting Parameters Revealed by Forced Chromatin Looping. Molecular Cell(62), 237, 2016.

Lee K, Hsiung CC, Huang P, Raj A, Blobel GA: Dynamic enhancer-gene body contacts during transcription elongation. Genes and Development 29: 1992, 2015.

Deng W, Rupon JW, Krivega I, Breda L, Motta I, Jahn KS, Reik A, Gregory PD, Rivella S, Dean A, Blobel GA: Reactivation of developmentally silenced globin genes by forced chromatin looping. Cell 158(4): 849, 2014.

Kadauke S, Udugama MI, Pawlicki JM, Achtman JC,Jain DP, Cheng Y, Hardison RC, and Blobel GA: Tissue-specific Mitotic Bookmarking by Hematopoietic Transcription Factor GATA1. Cell 150: 725, 2012.

Deng W, Lee J, Wang H, Miller J, Reik A, Gregory PD, Dean A, Blobel GA: Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. Cell 149: 1233, 2012.

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Last updated: 06/27/2018
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