Carl H. June, M.D.
Professor of Pathology and Laboratory Medicine
Department: Pathology and Laboratory Medicine
Graduate Group Affiliations
Contact information
554 BRB II/III
421 Curie Blvd.
Philadelphia, PA 19104
421 Curie Blvd.
Philadelphia, PA 19104
Office: (215) 573-5745
Fax: (215) 573-8590
Fax: (215) 573-8590
Email:
cjune@exchange.upenn.edu
cjune@exchange.upenn.edu
Publications
Links
Search PubMed for articles
Immunology graduate group faculty webpage.
Translational Research Programs, Abramson Cancer Center
Cell and Molecular Biology graduate group faculty webpage.
Search PubMed for articles
Immunology graduate group faculty webpage.
Translational Research Programs, Abramson Cancer Center
Cell and Molecular Biology graduate group faculty webpage.
Education:
B.S. (Biology)
United States Naval Academy , 1971.
M.D.
Baylor College of Medicine, 1979.
Permanent linkB.S. (Biology)
United States Naval Academy , 1971.
M.D.
Baylor College of Medicine, 1979.
Description of Research Expertise
Research Interestshuman T cell biology: mechanisms of lymphocyte costimulation.
Key words: T cells, human, gene therapy, genetic engineering, signal transduction.
Description of Research
There are two themes in the laboratory. One project involves determining mechanisms of lymphocyte activation. A more recent focus is to develop and test novel forms of immunotherapy for cancer and chronic infections. In the area of T cell signal transduction, for the past ten years these studies have been focused on CD28 and CTLA-4. More recently we have also been studying mechanisms that regulate chemokine receptor expression and signal transduction in T cells. In addition, we are studying the role of telomerase expression in telomere maintenance and replicative senescence in T cells. Novel lentiviral expression systems are permitting mechanistic studies of T cell costimulation in primary T cells for the first time.
Over the last five years my laboratory has been studying the potential use of adoptive immunotherapy for cancer and HIV infection. We have developed a large-scale tissue culture technique that permits the efficient propagation of polyclonal HIV CD4 and CD8 T cell subsets. Several clinical trials involving adoptive immunotherapy of autologous and allogeneic T cells are in process. Two trials are using genetically engineered T cells.
Rotation Projects for 2006-2007
1. mechanisms of T cell costimulation, studies using shRNA and lentiviral vectors
2. lentiviral vectors to create redirected regulatory T cells
3. mechanisms of superagonist T cell and NK cell signal transduction
Lab personnel:
Elena Perez, MD, PhD, Post Doc
Michael Milone, MD, PhD, Post Doc
Nicole Aqui, MD, Post Doc
Carmine Carpenito, MD, Post Doc
Megan Suhoski, graduate student
Selected Publications
Rapoport Aaron P, Stadtmauer Edward A, Aqui Nicole, Vogl Dan, Chew Anne, Fang Hong-Bin, Janofsky Stephen, Yager Kelly, Veloso Elizabeth, Zheng Zhaohui, Milliron Todd, Westphal Sandra, Cotte Julio, Huynh Hong, Cannon Andrea, Yanovich Saul, Akpek Gorgun, Tan Ming, Virts Kristen, Ruehle Kathleen, Harris Carolynn, Philip Sunita, Vonderheide Robert H, Levine Bruce L, June Carl H: Rapid immune recovery and graft-versus-host disease-like engraftment syndrome following adoptive transfer of Costimulated autologous T cells. Clinical Cancer Research 15(13): 4499-507, Jul 2009.Riley James L, June Carl H, Blazar Bruce R: Human T regulatory cell therapy: take a billion or so and call me in the morning. Immunity 30(5): 656-65, May 2009.
Wang Gary P, Levine Bruce L, Binder Gwendolyn K, Berry Charles C, Malani Nirav, McGarrity Gary, Tebas Pablo, June Carl H, Bushman Frederic D: Analysis of lentiviral vector integration in HIV+ study subjects receiving autologous infusions of gene modified CD4+ T cells. Molecular Therapy 17(5): 844-50, May 2009.
Milone MC, Fish JD, Carpenito C, Carroll RG, Binder GK, Teachey D, Samanta M, Lakhal M, Gloss B, Danet-Desnoyers G, Campana D, Riley JL, Grupp SA, June CH: Chimeric Receptors Containing CD137 Signal Transduction Domains Mediate Enhanced Survival of T Cells and Increased Antileukemic Efficacy In Vivo. Molecular therapy : the journal of the American Society of Gene Therapy Apr 21 Epub ahead of print 2009 Notes: PMID: 1938429.
Carpenito Carmine, Milone Michael C, Hassan Raffit, Simonet Jacqueline C, Lakhal Mehdi, Suhoski Megan M, Varela-Rohena Angel, Haines Kathleen M, Heitjan Daniel F, Albelda Steven M, Carroll Richard G, Riley James L, Pastan Ira, June Carl H: Control of large, established tumor xenografts with genetically retargeted human T cells containing CD28 and CD137 domains. Proceedings of the National Academy of Sciences of the United States of America 106(9): 3360-5, Mar 2009.
Varela-Rohena, A, Molloy, PE, Dunn, SM, Li, Y, Suhoski, MM, Carroll, RG, Milicic, A, Mahon, T, Sutton, DH, Laugel, B, Moysey, R, Cameron, BJ, Vuidepot, A, Purbhoo, MA, Cole, DK, Phillips, RE, June, CH, Jakobsen, BK, Sewell, AK, Riley, JL: Control of HIV-1 immune escape by CD8 T cells expressing enhanced T-cell receptor. Nature Medicine 14(12): 1390-1395, Dec 2008.
Carroll,R.G., Carpenito,C., Shan,X., net-Desnoyers,G., Liu,R., Jiang,S., Albelda,S.M., Golovina,T., Coukos,G., Riley,J.L., Jonak, Z.L., June, C.H.: Distinct effects of IL-18 on the engraftment and function of human effector CD8 T cells and regulatory T cells PLoS ONE 3(9): e3289-98, Oct 2008.
Aqui Nicole A, June Carl H: Post-transplant adoptive T-cell immunotherapy. Best practice & research. Clinical haematology 21(3): 503-19, Sep 2008.
Golovina TN, Mikheeva T, Suhoski MM, Aqui NA, Tai VC, Shan X, Liu R, Balcarcel RR, Fisher N, Levine BL, Carroll RG, Warner N, Blazar BR, June CH, Riley JL.: CD28 costimulation is essential for human T regulatory expansion and function. J Immunol. 181 (4): 2855-68, Aug. 2008.
Suhoski MM, Perez EE, Heltzer ML, Laney A, Shaffer LG, Saitta S, Nachman S, Spinner NB, June CH, Orange JS.: Monosomy 1p36 uncovers a role for OX40 in survival of activated CD4+ T cells. Clin Immunol. 128: 181-9, Aug. 2008.