Kathryn E. Hamilton, PhD

faculty photo
Assistant Professor of Pediatrics
Department: Pediatrics
Graduate Group Affiliations

Contact information
902F Abramson Research Center
3615 Civic Center Blvd
Children's Hospital of Philadelphia
Philadelphia, PA 19104
Lab: 267-426-5266
Education:
BA (Biology)
Assumption College, 2003.
PhD (Cell and Molecular Physiology)
University of North Carolina Chapel Hill, 2010.
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Description of Research Expertise

Research Interests: The Hamilton laboratory specializes in understanding mechanisms regulating gut homeostasis in health and disease, with a specific interest in epithelial cell responses to damage and inflammation. We employ in vivo models and primary, three-dimensional enteroid and colonoid cultures from mice and humans to understand the molecular basis of Inflammatory Bowel Disease (IBD) and colorectal cancer. Within these contexts, we are interested in how RNA binding proteins impart global, post-transcriptional regulation of key pathways in normal physiology and pathophysiology.

Keywords: gastrointestinal biology, RNA binding proteins, Inflammatory Bowel Disease (IBD), colorectal cancer, organoids (enteroids/colonoids), in vivo models

Research Details: Our laboratory incorporates training in cell biology, physiology/pathophysiology, and molecular genetics to understand basic mechanisms in human disease, providing foundations for new biomarkers or therapeutic targets. Techniques employed in the laboratory include tissue culture (2D and 3D), molecular biology, microscopy, immunohistochemistry, flow cytometry and in vivo disease models. New techniques being incorporated into the laboratory in the coming months include transcriptomics, ribonomics, and proteomics to understand global changes in the context of deleting or overexpressing RNA binding proteins.

Rotation Projects:
1) Dissecting regulation of the autophagy pathway by the RNA binding protein IMP1 in 3D colonoids
2) Evaluating specific inflammatory factors regulating the RNA binding protein IMP1 and response to experimental colitis
3) Determining RNA binding protein "regulons" in adult Inflammatory Bowel Disease
4) Evaluating novel pathways regulated by IMP1 in pediatric, VEO (Very Early Onset)-IBD
5) Evaluating the role of RNA binding protein IMP1 overexpression in amplifying or suppressing key oncogenes in the progression of inflammation-associated colorectal cancer

Lab Personnel:
Lauren Simon, BS, Research Specialist
Rithika Behera, BS, Penn Master's in Biotechnology student
David Nguyen, Penn undergraduate student

Selected Publications

Apple G. Long, Emma T. Lundsmith, Kathryn E. Hamilton: Inflammation and Colorectal Cancer. Current Colorectal Cancer Reports. Springer US, 13(4), Aug 2017.

Kelsen JR, Conrad MA, Simon LA, Williams KN, Mizuno R, Chatterji P, Dawany N, Giraudo C, Behrens EM, Sullivan KE, Devoto M, Hamilton KE : Using functional assays in patient-derived enteroids to understand genotype-phenotype interactions in Very Early Onset Inflammatory Bowel Disease (VEO-IBD) (Poster Presentation) Digestive Diseases Week May 2017 Notes: Chicago, IL.

Dana Almohazey, Yuan-Hung Lo, Claire Vossler, Alan Simmons, Jonathan Hsieh, Edie Bucar, Michael Schumacher, Kathryn Hamilton, Ken Lau, Noah Shroyer, Mark Frey: The ErbB3 receptor tyrosine kinase negatively regulates Paneth cells by PI3K-dependent suppression of Atoh1. Cell Death and Differentiation March 2017 Notes: In press.

Giroux V, Lento AA, Islam M, Kharbanda A, Pitaressi JR, Hamilton KE, Whelan KA, Long A, Rhoades B, Tang Q, Nakagawa H, Lengner CJ, Bass AJ, Wileyto EP, Klein-Szanto AJ, Wang TC, Rustgi AK : Novel long-lived esophageal progenitor cells contribute to homeostasis and regeneration. Journal of Clinical Investigation March 2017 Notes: In Press.

Whelan Kelly A, Merves Jamie F, Giroux Veronique, Tanaka Koji, Guo Andy, Chandramouleeswaran Prasanna M, Benitez Alain J, Dods Kara, Que Jianwen, Masterson Joanne C, Fernando Shahan D, Godwin Bridget C, Klein-Szanto Andres J, Chikwava Kudakwashe, Ruchelli Eduardo D, Hamilton Kathryn E, Muir Amanda B, Wang Mei-Lun, Furuta Glenn T, Falk Gary W, Spergel Jonathan M, Nakagawa Hiroshi: Autophagy mediates epithelial cytoprotection in eosinophilic oesophagitis. Gut Feb 2016 Notes: doi: 10.1136/gutjnl-2015-310341. [Epub ahead of print]

Hamilton Kathryn E, Chatterji Priya, Lundsmith Emma T, Andres Sarah F, Giroux Veronique, Hicks Philip D, Noubissi Felicite K, Spiegelman Vladimir S, Rustgi Anil K: Loss of Stromal IMP1 Promotes a Tumorigenic Microenvironment in the Colon. Molecular Cancer Research 13(11): 1478-86, Nov 2015.

Hamilton Kathryn E, Crissey Mary Ann S, Lynch John P, Rustgi Anil K: Culturing adult stem cells from mouse small intestinal crypts. Cold Spring Harbor protocols 2015(4): 354-8, Apr 2015.

Amaravadi Ravi K, Hamilton Kathryn E, Ma Xiaohong, Piao Shengfu, Portillo Armando Del, Nathanson Katherine L, Carlino Matteo S, Long Georgina V, Puzanov Igor, Xu Xiaowei, Morrissette Jennifer J D, Tsai Kenneth Y, Flaherty Keith T, Sosman Jeffrey A, Goodman Grant R, McArthur Grant A, Rustgi Anil K, Metz David C, Schuchter Lynn M, Chapman Paul B, Sepulveda Antonia R: Multiple Gastrointestinal Polyps in Patients Treated with BRAF Inhibitors. Clinical Cancer Research 21(23): 5215-21, Dec 2015.

Hamilton Kathryn E, Noubissi Felicite K, Katti Prateek S, Hahn Christopher M, Davey Sonya R, Lundsmith Emma T, Klein-Szanto Andres J, Rhim Andrew D, Spiegelman Vladimir S, Rustgi Anil K: IMP1 promotes tumor growth, dissemination and a tumor-initiating cell phenotype in colorectal cancer cell xenografts. Carcinogenesis 34(11): 2647-54, Nov 2013.

Hamilton Kathryn E, Simmons James G, Ding Shengli, Van Landeghem Laurianne, Lund P Kay: Cytokine induction of tumor necrosis factor receptor 2 is mediated by STAT3 in colon cancer cells. Molecular Cancer Research 9(12): 1718-31, Dec 2011.

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Last updated: 10/18/2017
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