Judith B. Grinspan, Ph.D.
Research Associate Professor of Neurology
Department: Neurology
Graduate Group Affiliations
Contact information
Children’s Hospital of Philadelphia
514 Abramson Center
Philadelphia, PA 19104
514 Abramson Center
Philadelphia, PA 19104
Office: (215) 590-2094
Fax: (215) 590-3709
Fax: (215) 590-3709
Email:
GRINSPAN@EMAIL.CHOP.EDU
GRINSPAN@EMAIL.CHOP.EDU
Publications
Links
Search PubMed for articles
Cell and Molecular Biology graduate group faculty webpage.
Neuroscience graduate group faculty webpage.
Search PubMed for articles
Cell and Molecular Biology graduate group faculty webpage.
Neuroscience graduate group faculty webpage.
Education:
A.B. (Biology)
Vassar College , 1974.
M.S. (Pathology)
Hahnemann University , 1977.
Ph.D. (Biology)
University of Pennsylvania, 1984.
Permanent linkA.B. (Biology)
Vassar College , 1974.
M.S. (Pathology)
Hahnemann University , 1977.
Ph.D. (Biology)
University of Pennsylvania, 1984.
Description of Research Expertise
Research InterestsOur lab studies the controls of the development of oligodendrocytes, the myelinating cells of the central nervous system, from stem cells through to myelination.
Key words: oligodendrocytes, myelin, growth factors, programmed cell death, hypoxia/ischemia.
Description of Research
In the central nervous system, oligodendrocytes synthesize myelin as an extension of their plasma membranes. This myelin wraps axons and allows rapid and efficient conduction of nervous impulses. Destruction of myelin through injury, such as birth injury leading to cerebral palsy, or disease, such as multiple sclerosis, causes extreme loss of function. Oligodendrocyte precursors and stem cells remain in the CNS following the pathology and are potentially capable of forming mature oligodendrocytes and then myelin. However, their maturation is severely limited. Possible reasons for this include the inhibitors present in the area that impede maturation and astrogliosis in which astrocytes form physical and chemical barriers to regeneration. Our goal is to identify factors in the CNS that promote or inhibit the development of mature oligodendrocytes both during development and disease. On going areas of investigation in the lab include: The role and interactions of dorsal signaling factors, bone morphogenetic proteins (BMPs) and Wnts, in the development of oligodendrocytes and myelination. -The fate of oligodendrocyte maturation and myelination following hypoxic/ischemic injury in the central nervous system. The role of BMPs in oxidative stress following birth injury or demyelination in the adult. Rotation projects for 2010-2011: 1) The role of BMP in a model of dysmyelinating disease. 2) The role of intermediates such as NF-kB and iNOS in oxidative stress during oligodendrocyte maturation. 3)The role of dorsal signaling factors and reactive oxygen species in oligodendrocyte:neuronal co-cultures. 3)BMP regulation of oligodendrocyte development by non-canonical signaling pathways.
Lab personnel:
Mary Reid research assistant
Keith Feigenson graduate student
Selected Publications
French, H. M., Reid, M., Mamontov, P., Simmons, R. A., Grinspan, J. B.: Oxidative stress disrupts oligodendrocyte maturation. Journal of Neuroscience Research 87: 3076-3087, 2009.Feigenson, K., Reid, M., See, J., Crenshaw E.B. III, Grinspan, J. B.: Wnt signaling is sufficient to perturb oligodendrocyte maturation. Molecular and Cellular Neuroscience 42: 255-265, 2009.
Marek, R., Caruso, M. ,Rostami, A.M., Grinspan, J.B., Das Sarma, J.: CD11b magnetic cell sorting: a fast and effective method of concurrent isolation of high purity viable astrocytes and microglia from neonatal mouse brain tissue. J Neuroscience Methods 175: 108-118, 2008.
Ara, J., See, J., Mamontov, P., Hahn, A., Bannerman, P. Pleasure, D., Grinspan, J.B.: Bone morphogenetic proteins 4, 6, and7 are upregulated in mouse spinal cord during experimental autoimmune encephalomyelitis. Journal of Neuroscience Research 86: 125-135, 2008.
See, J., Mamontov, P., Ahn K., Wine-Lee, L., Crenshaw III, E.B., Grinspan, J. B.: BMP mutant mice exhibit glial cell maturation defects. Molecular and Cellular Neuroscience 35: 171-182, 2007.
Huang, Y., Grinspan, J.B, , Abrams, C. K., Scherer, S. S.: Pannexin 1 is expressed by neurons and glia but does not form functional gap junctions. Glia 55: 46-56, 2007.
Devaux, J., Alcaraz, G., Grinspan, J., Bennett, V., Joho, R., Crest, M., Scherer, S. : Kv3.1b is a novel component of CNS nodes. J. Neurosci 23: 4509-4518, 2003.
See, J., Zhang, X., Eraydin, N. Mun, S.B., Mamontov, P., Golden, J. Grinspan, J.B.: Oligodendrocyte maturation is inhibited by bone morphogenetic protein. Molecular and Cellular Neuroscience 26: 481-492, 2004.
Tang, X.-M., Beesley, J.S., Grinspan, J.B., Seth, P., Kamholz, J. and Cambi, F. : Cell cycle arrest induced by extopic expression of p27 is not sufficient to promote oligodendrocyte differentiation. J. Cell. Biochem. 76: 270-279, 2000.
Golden, J.A., Bracilovic, A., McFadden, K.A., Beesley, J.S., Rubenstein, L.R. and Grinspan, J.B. : Ectopic BMP4 in the chick forebrain leads to cyclopia and holoprosencephaly. Proc. Natl. Acad. Sci. USA 96: 2439-2444, 1999.