Penn Medicine
Help | Search | Site Map | Contact   
faculty photo

Hajime Takano, Ph.D.

Research Assistant Professor of Neurology
Department: Neurology

Contact information
The Children’s Hospital of Philadelphia
Abramson Research Center
3615 Civic Center Boulevard, Room 409
Philadelphia, PA 19104
Office: 2155901744
Education:
B.S. (BiomolecularEngineering)
Tokyo Institute of Technology, Japan , 1992.
M.S. (Bioengineering/Biotechnology)
Tokyo Institute of Technology, Japan , 1994.
Ph.D. (Bioengineering/Biotechnology)
Tokyo Institute of Technology, Japan , 1997.
Post-Graduate Training
Postdoctoral Research Associate, Microanalytical Instrumentation Center, Analytical Chemistry, Iowa State University, Professor Marc D. Porter , 1997-2000.
Postdoctoral Research Associate, Roy J. Carver Laboratory for Ultrahigh Resolution Biological Microscopy, Iowa State University,Professor Marc D. Porter,Professor Philip G Haydon , 2000-2001.
Permanent link
 
> Perelman School of Medicine   > Faculty   > Details

Description of Research Expertise

Technology Development
• High speed calcium imaging of neuronal networks and related data analysis
• Integration of fluorescence lifetime measurement system on two photon microscopy
• Quantitative image analysis for massive imaging data (>10,000 images)
• Integrating a high speed camera into two photon microscopy
• Building optical stimulation devices using digital mirror arrays
• Protocol development for ATP imaging with a photon counting CCD camera
• Building a scanning near-field optical microscopy
• Microfabrication of cell culture platforms for studying inter-cellular signaling
• Developing subsurface imaging tool using scanning probe microscopy

Advanced Microscopy
Teach and instruct advanced imaging technique including multi-photon microscopy, second harmonic generation, laser scanning confocal microscopy, fluorescence microscopy, fluorescence spectra, FRET, FRAP, scanning probe microscopy (AFM, EFM, FFM, SNOM) for chemical and biological analysis.

Programming - Image Analysis, Data Analysis, Statistical Analysis
Labview(instrumentation), Matlab(data and image analysis, GUI)

Microfabrication and Nanotechnology
BioMEMS, Softlithography, Microcontact Printing; expert on fabricating and characterizing self-assembled monolayer, polymer thin films, Langmuir-Blodgett films, and immobilized proteins on the solid surfaces.

Analytical Chemistry
HPLC, electrochemically modulated liquid chromatography (EMLC), EMLC-mass spectroscopy detection, FT-IR

Selected Publications

Yu, E., Dengler, C., Frausto, S., Putt, M., Yue, C., Takano, H., and Coulter, D.A.: Protracted postnatal development of sparse, specific dentate granule cell activation in the mouse hippocampus. Journal of Neuroscience 33(7): 2947-2960, 2013.

Takano, H., McCartney, M., Ortinski, P., Putt, M., and Coulter, D.A.: Deterministic and stochastic neuronal contributions to distinct synchronous CA3 network bursts. Journal of Neuroscience 32(14): 4743-4754, 2012.

Takano, H.,Coulter, D.A.: Imaging of hippocampal circuits in epilepsy. Jasper’s Basic Mechanisms of Epilepsies, 3rd Edition. Noebeis, J., Rogawski, M., Delgado-Escueta, A., Avoli, M. (eds.). 2011.

Ortinski, P. I., Dong, J., Mungenast, A., Yue, C., Takano, H., Watson, D. J., Haydon, P. G., Coulter, D. A.: Selective induction of astrocytic gliosis generates deficits in neuronal inhibition. Nat Neurosci 13(5): 584-91, 2010.

Halassa, M. M., Fellin, T., Takano, H., Dong, J. H., Haydon, P. G.: Synaptic islands defined by the territory of a single astrocyte. J Neurosci 27(24): 6473-7, 2007.

Barrett, L. E., Sul, J. Y., Takano, H., Van Bockstaele, E. J., Haydon, P. G., Eberwine, J. H.: Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor. Nat Methods 3(6): 455-60, 2006.

Mrass, P., Takano, H., Ng, L. G., Daxini, S., Lasaro, M. O., Iparraguirre, A., Cavanagh, L. L., von Andrian, U. H., Ertl, H. C., Haydon, P. G., Weninger, W.: Random migration precedes stable target cell interactions of tumor-infiltrating T cells. J Exp Med 203(12): 2749-61, 2006.

Takano, H, Sul, JY, Mazzanti Y, Doyle, RT, Haydon, PG and Porter,MD: Micropatterned substrates: approach to probing intercellular communication pathways. Analytical Chemistry 74(18): 4640-4646, 2002.

Takano, H., Porter, M. D.: Monitoring chemical transformations at buried organic interfaces by electric force microscopy. J Am Chem Soc 123(34): 8412-3, 2001.

Takano,H., Kenseth,J.R., Wong, S.S., O'Brien,J.C., Porter, M.D.: Chemical and Biological Analysis using Scanning Force Microscopy. Chemical Review 99: 2845-2890, 1999.

Deng, H., Van Berkel, G. J., Takano, H., Gazda, D., Porter, M. D.: Electrochemically modulated liquid chromatography coupled on-line with electrospray mass spectrometry. Analytical Chemistry 72(11): 2641-7, 2000.

Takano, H., Fujihira, M.: AFM microlithography of a thin chromium film covered with a thin resist Langmuir-Blodgett (LB) film. Thin Solid Films 273(1-2): 312-316, 1996.

Overney, R. M., Meyer, E., Frommer, J., Brodbeck, D., Luthi, R., Howald, L., Guntherodt, H. J., Fujihira, M., Takano, H., Gotoh, Y.: Friction measurements on phase-separated thin-films with a modified atomic force microscope. Nature 359(6391): 133-135, 1992.

back to top
Last updated: 10/20/2017
The Trustees of the University of Pennsylvania
 
© The Trustees of the University of Pennsylvania. Site best viewed with Mozilla, NN, IE, or Safari.
SOM Home International Programs Penn Alumni Clinical Trials Centers and Institutes Departments Faculty Research Education Administration