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Hajime Takano, Ph.D.

Research Assistant Professor of Neurology
Department: Neurology

Contact information
The Children’s Hospital of Philadelphia
Abramson Research Center
3615 Civic Center Boulevard, Room 409
Philadelphia, PA 19104
Office: 2155901744
B.S. (BiomolecularEngineering)
Tokyo Institute of Technology, Japan , 1992.
M.S. (Bioengineering/Biotechnology)
Tokyo Institute of Technology, Japan , 1994.
Ph.D. (Bioengineering/Biotechnology)
Tokyo Institute of Technology, Japan , 1997.
Post-Graduate Training
Postdoctoral Research Associate, Microanalytical Instrumentation Center, Analytical Chemistry, Iowa State University, Professor Marc D. Porter , 1997-2000.
Postdoctoral Research Associate, Roy J. Carver Laboratory for Ultrahigh Resolution Biological Microscopy, Iowa State University,Professor Marc D. Porter,Professor Philip G Haydon , 2000-2001.
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Description of Research Expertise

Technology Development
• High speed calcium imaging of neuronal networks and related data analysis
• Integration of fluorescence lifetime measurement system on two photon microscopy
• Quantitative image analysis for massive imaging data (>10,000 images)
• Integrating a high speed camera into two photon microscopy
• Building optical stimulation devices using digital mirror arrays
• Protocol development for ATP imaging with a photon counting CCD camera
• Building a scanning near-field optical microscopy
• Microfabrication of cell culture platforms for studying inter-cellular signaling
• Developing subsurface imaging tool using scanning probe microscopy

Advanced Microscopy
Teach and instruct advanced imaging technique including multi-photon microscopy, second harmonic generation, laser scanning confocal microscopy, fluorescence microscopy, fluorescence spectra, FRET, FRAP, scanning probe microscopy (AFM, EFM, FFM, SNOM) for chemical and biological analysis.

Programming - Image Analysis, Data Analysis, Statistical Analysis
Labview(instrumentation), Matlab(data and image analysis, GUI)

Microfabrication and Nanotechnology
BioMEMS, Softlithography, Microcontact Printing; expert on fabricating and characterizing self-assembled monolayer, polymer thin films, Langmuir-Blodgett films, and immobilized proteins on the solid surfaces.

Analytical Chemistry
HPLC, electrochemically modulated liquid chromatography (EMLC), EMLC-mass spectroscopy detection, FT-IR

Selected Publications

Yu, E., Dengler, C., Frausto, S., Putt, M., Yue, C., Takano, H., and Coulter, D.A.: Protracted postnatal development of sparse, specific dentate granule cell activation in the mouse hippocampus. Journal of Neuroscience 33(7): 2947-2960, 2013.

Takano, H., McCartney, M., Ortinski, P., Putt, M., and Coulter, D.A.: Deterministic and stochastic neuronal contributions to distinct synchronous CA3 network bursts. Journal of Neuroscience 32(14): 4743-4754, 2012.

Takano, H.,Coulter, D.A.: Imaging of hippocampal circuits in epilepsy. Jasper’s Basic Mechanisms of Epilepsies, 3rd Edition. Noebeis, J., Rogawski, M., Delgado-Escueta, A., Avoli, M. (eds.). 2011.

Ortinski, P. I., Dong, J., Mungenast, A., Yue, C., Takano, H., Watson, D. J., Haydon, P. G., Coulter, D. A.: Selective induction of astrocytic gliosis generates deficits in neuronal inhibition. Nat Neurosci 13(5): 584-91, 2010.

Halassa, M. M., Fellin, T., Takano, H., Dong, J. H., Haydon, P. G.: Synaptic islands defined by the territory of a single astrocyte. J Neurosci 27(24): 6473-7, 2007.

Barrett, L. E., Sul, J. Y., Takano, H., Van Bockstaele, E. J., Haydon, P. G., Eberwine, J. H.: Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor. Nat Methods 3(6): 455-60, 2006.

Mrass, P., Takano, H., Ng, L. G., Daxini, S., Lasaro, M. O., Iparraguirre, A., Cavanagh, L. L., von Andrian, U. H., Ertl, H. C., Haydon, P. G., Weninger, W.: Random migration precedes stable target cell interactions of tumor-infiltrating T cells. J Exp Med 203(12): 2749-61, 2006.

Takano, H, Sul, JY, Mazzanti Y, Doyle, RT, Haydon, PG and Porter,MD: Micropatterned substrates: approach to probing intercellular communication pathways. Analytical Chemistry 74(18): 4640-4646, 2002.

Takano, H., Porter, M. D.: Monitoring chemical transformations at buried organic interfaces by electric force microscopy. J Am Chem Soc 123(34): 8412-3, 2001.

Takano,H., Kenseth,J.R., Wong, S.S., O'Brien,J.C., Porter, M.D.: Chemical and Biological Analysis using Scanning Force Microscopy. Chemical Review 99: 2845-2890, 1999.

Deng, H., Van Berkel, G. J., Takano, H., Gazda, D., Porter, M. D.: Electrochemically modulated liquid chromatography coupled on-line with electrospray mass spectrometry. Analytical Chemistry 72(11): 2641-7, 2000.

Takano, H., Fujihira, M.: AFM microlithography of a thin chromium film covered with a thin resist Langmuir-Blodgett (LB) film. Thin Solid Films 273(1-2): 312-316, 1996.

Overney, R. M., Meyer, E., Frommer, J., Brodbeck, D., Luthi, R., Howald, L., Guntherodt, H. J., Fujihira, M., Takano, H., Gotoh, Y.: Friction measurements on phase-separated thin-films with a modified atomic force microscope. Nature 359(6391): 133-135, 1992.

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Last updated: 10/20/2017
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