Olfaction and Respiration-driven Brain Activity
Patch clamp, Optical imaging, Optogenetics, Electrophysiology in behaving and anesthetized mice, Neural circuit mapping, Mouse behavior, Immunohistochemistry, Molecular biology
The mammalian olfactory system relies on a large number (~1200 in rodents) of G-protein coupled odorant receptors (ORs) to detect and discriminate numerous odors in the environment. Each olfactory sensory neuron (OSN) in the nose manages to express only one OR type. By combining patch clamp, optical imaging, molecular, and genetic approaches, we are investigating how the olfactory sensory neurons with defined ORs encode different odors in the environment and how olfactory experience modifies the sensitivity and organization of the system.
We recently discovered that OSNs serve dual functions as odor detectors and mechanical sensors, which leads to the hypothesis that the nose provides the respiration rhythm to the brain in addition to the olfactory information. Respiration is not only influenced by autonomic demands, but also by emotional states (fear, anxiety, anger, etc). On the other hand, conscious control of breathing, usually in the form of rhythmic nasal breathing, can significantly affect physiology, mood, and cognition. By combining optogenetics, electrophysiological recordings in behaving and anesthetized animals, behaviors, and neural circuit mapping, we are investigating the source and significance of respiration-locked brain activity.
Yu Y†, de March CA†, Ni MJ, Adipietro KA, Golebiowski J*, Matsunami H*, Ma M* : Responsiveness of G protein-coupled odorant receptors is partially attributed to the activation mechanism. Proc Natl Acad Sci 112: 14966-14971, 2015. Notes: †equal contribution and *corresponding authors.
Challis RC, Tian H, Wang J, He J, Jiang J, Chen X, Yin W, Connelly T, Ma L, Yu CR, Pluznick JL, Storm DR, Huang L, Zhao K, Ma M: An olfactory cilia pattern in the mammalian nose ensures high sensitivity to odors. Curr Biol 25: 2503-2512, 2015. Notes: Commented by Wall CM and Zhao H (2015) Sensory Biology: Novel Peripheral Organization for Better Smell. Curr Biol. 25:R833-6.
de March CA†, Yu Y†, Ni MJ, Adipietro KA, Matsunami H*, Ma M*, Golebiowski J*: Conserved residues control activation of mammalian G Protein-coupled odorant receptors. J Am Chem Soc 137: 8611-8616, 2015. Notes: †equal contribution and *corresponding authors.
Jiang Y†, Li YR†, Tian H, Ma M*, Matsunami H*: Muscarinic acetylcholine receptor M3 modulates odorant receptor activity via inhibition of β-Arrestin-2 recruitment. Nature Commun 6: 6448 (1-15), 2015. Notes: †equal contribution and *corresponding authors.
Connelly T†, Yu Y†, Grosmaitre X, Wang J, Santarelli LC, Savigner A, Qiao X, Wang Z, Storm DR, Ma M: G Protein-coupled odorant receptors underlie mechanosensitivity in mammalian olfactory sensory neurons. Proc Natl Acad Sci 112: 590-5, 2015. Notes: †equal contribution.
Lee AC, He J and Ma M: Olfactory Marker Protein Is Critical for Functional Maturation of Olfactory Sensory Neurons and Development of Mother Preference. J Neurosci 31: 2974–2982, 2011.
Tan J, Savigner A, Ma M and Luo M: Odor information processing by the olfactory bulb analyzed in gene-targeted mice. Neuron 65: 912-926, 2010.
Grosmaitre X, Fuss SH, Lee AC, Adipietro KA, Matsunami H, Mombaerts P and Ma M: SR1, a mouse odorant receptor with an unusually broad response profile. J Neurosci 29: 14545-14552, 2009.
Grosmaitre X, Santarelli LC, Tan J, Luo M and Ma M: Dual functions of mammalian olfactory sensory neurons as odor detectors and mechanical sensors. Nature Neurosci 10: 348-354, 2007. Notes: Featured in Research Highlights in Nature (March 1, 2007), 446:5.
Grosmaitre X, Vassalli A, Mombaerts P, Shepherd GM and Ma M: Odorant responses of olfactory sensory neurons expressing the odorant receptor MOR23: a patch clamp analysis in gene-targeted mice. Proc Natl Acad Sci 103: 1970-1975, 2006. Notes: Featured in “In This Issue” by PNAS.
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Last updated: 07/19/2016
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