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Faculty Members:
Greg J. Bashaw, Ph.D.
Associate Professor of Neuroscience
Present Member
Department: Neuroscience
Contact information
1113 BRB II/III Building
Department of Neuroscience
421 Curie Blvd.
Philadelphia, PA 19104
Department of Neuroscience
421 Curie Blvd.
Philadelphia, PA 19104
Office: 215 898-0829
Fax: 215 573-7601
Fax: 215 573-7601
Email:
gbashaw@mail.med.upenn.edu
gbashaw@mail.med.upenn.edu
Graduate Group Affiliations
Publications
Links
Search PubMed for articles
Cell and Molecular Biology graduate group webpage.
Neuroscience graduate group faculty webpage.
Search PubMed for articles
Cell and Molecular Biology graduate group webpage.
Neuroscience graduate group faculty webpage.
Education:
B.A. (Biology)
Brown University, 1990.
Ph.D. (Biological Sciences)
Stanford University, 1997.
B.A. (Biology)
Brown University, 1990.
Ph.D. (Biological Sciences)
Stanford University, 1997.
Post-Graduate Training
Post-doctoral Fellow, Molecular and Cell Biology, University of California, Berkeley, 1998-2001.
Permanent linkPost-doctoral Fellow, Molecular and Cell Biology, University of California, Berkeley, 1998-2001.
Description of Research Expertise
Research Interests- Molecular mechanisms of axon growth and guidance during nervous system development.
- How axon guidance receptors specify attractive and repulsive signals and transmit these signals to the navigating growth cone to generate a directed motile response.
Key words: Axon guidance, developmental neuroscience, Slit, Robo, Netrin.
Description of Research
How axons in the developing nervous system successfully navigate to their correct targets is a fundamental problem in neurobiology. Understanding the mechanisms that mediate axon guidance will give important insight into how the nervous system is correctly wired during development and may have implications for therapeutic approaches to developmental brain disorders and nerve regeneration. Achieving this understanding will require unraveling the molecular logic that ensures the proper expression and localization of axon guidance cues and receptors, and elucidating the signaling events that regulate the growth cone cytoskeleton in response to guidance receptor activation.
The Slit ligand and Roundabout (Robo) receptors, and the Netrin ligand and DCC/UNC5 receptors are two important evolutionary conserved ligand/receptor systems that contribute to proper connectivity in both the vertebrate and invertebrate nervous systems. These molecules are also known to influence neuronal and mesodermal cell migration, suggesting that determining their function may have broad implications for understanding diseases of nervous system development, many of which have their root in defective cell migration and/or axon guidance. The research in my laboratory addresses the dynamics of axon guidance receptor expression and signaling, and exploits the powerful genetic and molecular approaches available in Drosophila.
Rotation Projects for 2009-2010
A range of projects relating to axon guidance receptor regulation and signaling, employing a broad range of techniques in genetics, molecular biology and biochemistry. For specific projects contact Dr. Bashaw.
Lab personnel:
Timothy Evans, Ph.D., Post-doctoral Fellow
Michael O'Donnell, CAMB Graduate Student
Rebecca Chance, Neuroscience Graduate Student
Alexandra Neuhaus-Follini, Neuroscience Graduate Student
Angela Jablonski, Neuroscience Rotation Student
Jeremy Vogelsong, Research Specialist
Selected Publications
Yang Long, Garbe David S, Bashaw Greg J: A frazzled/DCC-dependent transcriptional switch regulates midline axon guidance. Science 324(5929): 944-7, May 2009.Garbe, D.S., O’Donnell, M., and Bashaw, G.J.: Cytoplasmic Domain Requirements for Frazzled-mediated Attractive Axon Turning at the Drosophila Midline. Development 134: 4315-4324. December 2007.
David S. Garbe and Greg J. Bashaw: Independent Functions of Slit-Robo Repulsion and Netrin-Frazzled Attraction Regulate Axon Crossing at the Midline in Drosophila. Journal of Neuroscience 27(13): 3584-3592, March 2007.
Yang, L. and Bashaw, G.J.: Son of Sevenless directly links the Robo receptor to Rac activation to control axon repulsion at the midline. Neuron 52(4): 595-607, November 2006.
Garbe, D.S., Das, A., Dubreuil, R.R. and Bashaw, G.J.: Beta spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS. Development doi:10.1242(dev.02653), November 2006 Notes: Development ePress Electronic Publication ahead of Print.
Hu, H., Li, M., Labrador, J.P., McEwen, J., Lai, E.C., Goodman, C.S. and Bashaw, G.J.: Cross GAP/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. PNAS 102: 4613-4618, 2005.
Labrador, J.P., O'Keefe, D., Yoshikawa, S., McKinnon, R.D., Thomas, J.B. and Bashaw, G.J.: The homeobox transcription factor even-skipped regulates Netrin-receptor expression to control dorsal motor-axon projections in Drosophila. Current Biology 15: 1413-1419, 2005.
Fan, X., Labrador, J.P., Hing, H. and Bashaw, G.J.: Slit stimulation recruits Dock and Pak to the Roundabout receptor and increases Rac activity to regulate axon repulsion at the CNS midline. Neuron 41: 113-127, 2003.
Godenschwege, T.A., Simpson, J.H., Shan, X., Bashaw, G.J., Goodman, C.S. and Murphey, R.K.: Ectopic expression in the giant fiber system of Drosophila reveals distinct roles for roundabout (Robo), Robo2, and Robo3 in dendritic guidance and synaptic connectivity. Journal of Neuroscience 22(8): 3117-3129, April 15 2002.
Bashaw, G.J., Hu H., Nobes C.D. and Goodman, C.S.: A novel Dbl family RhoGEF promotes Rho-dependent axon attraction to the central nervous system midline in Drosophila and overcomes Robo repulsion. Journal of Cell Biology (Cover) 155(7): 1117-1122, December 24 2001.
