Hua-Ying Fan, Ph.D.
Assistant Professor of Biochemistry and Biophysics
Department: Biochemistry and Biophysics
Graduate Group Affiliations
Contact information
Department of Biochemistry and Biophysics
University of Pennsylvania School of Medicine
564 Clinical Research Building
415 Curie Boulevard
Philadelphia, PA
Philadelphia, PA 19104-6145
University of Pennsylvania School of Medicine
564 Clinical Research Building
415 Curie Boulevard
Philadelphia, PA
Philadelphia, PA 19104-6145
Office: 215 573-5705
Email:
hfan@mail.med.upenn.edu
hfan@mail.med.upenn.edu
Education:
B.S. (Chemistry)
National Tsing-Hua University, Hsin-Chu, Taiwan, 1989.
M.S. (Chemistry)
New York University, New York, NY, 1990.
Ph.D. (Cellular and Molecular Biology)
Sackler Institute of New York University Medical Center, New York University, New York, NY, 1996.
Permanent linkB.S. (Chemistry)
National Tsing-Hua University, Hsin-Chu, Taiwan, 1989.
M.S. (Chemistry)
New York University, New York, NY, 1990.
Ph.D. (Cellular and Molecular Biology)
Sackler Institute of New York University Medical Center, New York University, New York, NY, 1996.
Description of Research Expertise
The Fan lab is interested in understanding the mechanisms of epigenetic regulation. Epigenetics describes the process by which a specific transcriptional program, induced by a signal, is maintained and inherited through cell divisions without the necessary presence of the original signal. Chromatin structure holds the secrets of epigenetic regulation. To dissect the mechanisms of epigenetic regulation, our research is focused in two main directions. First, we study how ATP-dependent chromatin remodelers regulate chromatin structure and how defects in these activities can lead to disease. Currently, we are using CSB (Cockayne Syndrome complementation group B) as a model protein to understand the function of ATP-dependent chromatin remodeling in DNA repair, aging and cancer. Second, we wish to understand how epigenetic regulatory mechanisms influence cancer cell self-renewal and differentiation, mechanisms that might impact the programming and reprogramming capacity of cancer cells. Results from our studies will, therefore, not only shed light on fundamental mechanism of epigenetic regulation, but will provide novel insights into the causes and mechanisms of disease.Lab Personnel:
Il-Taeg Cho, Ph.D. Postdoctoral Researcher
Ju-Yeon Kim, Undergraduate student, Biology
Robert Lake, Ph.D. Senior Research Investigator
Glennis Logsdon, Rotation Student, BMB graduate program
Sandeep Patnaik, Undergraduate Student, Biochemistry
Surabhi Srinivasan, Graduate Student, Chemical and Biomolecular Engineering
Pei-Fang Tsai, Ph.D. Postdoctoral Researcher
Selected Publications
Lake, R.J., Basheer, A., and Fan, H.-Y.: Reciprocally regulated chromatin association of the Cockayne syndrome protein B and p53. J. Biol. Chem. 286(40): 34951-8, Oct 7 2011 Notes: Epub 2011 Aug 18.Lake RJ, Geyko A., Hemashettar G., Zhao Y. and Fan, H.-Y. : UV-induced association of the CSB remodeling protein with chromatin requires ATP-dependent relief of N-terminal autorepression. Molecular Cell 37: 235-246, 2010.
Kelly DF, Lake RJ, Middelkoop TC, Fan H-Y, Artavanis-Tsakonas S, and Waltz, T. : Molecular structure and dimeric organization of the Notch extracellular domain as revealed by electron microscopy. PLoS ONE 5: e10532. doi:10.1371/journal.pone.0010532 2010.
Lavigne M, Eskeland R, Azebi S, Saint-André V., Jang, S. M., Batsché,E., Fan, H.-Y., Kingston, R. E., Imhof, A., and Muchardt, C. : Interaction of HP1 and Brg1/Brm with the globular domain of histone H3 required for HP1-mediated repression. PLoS Genetics 5: e1000769. doi:10.1371/journal.pgen.1000769, 2009.
Trotter, K.W., Fan, H.-Y., Ivey, M., Kingston, R.E., Archer, T.K. : The HSA domain of BRG1 mediates critical interactions required for GR2 dependent transcriptional activation in vivo. Mol. Cell Biol. 28: 1413-1426, 2008.
Newman, J.C., Bailey, A.D., Fan, H.-Y., Pavelitz, T., and Weiner, A.M. : An abundant evolutionarily conserved CSB-PiggyBac fusion protein expressed in Cockayne syndrome. PLoS Genetics 4: e1000031, 2008.
He, X., Fan, H.-Y., Garlick, J.D., and Kingston, R.E. : Diverse regulation of SNF2h chromatin remodeling by noncatalytic subunits. Biochemistry 47: 7025-7033, 2008.
Zhang, Z., Fan, H.-Y., Goldman, J. A., and Kingston, R. E. : Homology driven chromatin remodeling by human Rad54. Nature Struct. Mol. Biol. 14: 397-405, 2007.
Dennis, J.H.*, Fan, H.-Y.*, Reynolds, M.S.*, Yuan, G., Meldrim, J, Richter, D.J., Peterson, D.G., Rando, O.J., Noble, W.S., Kingston R.E. (*These authors contribute equally to this work.): Independent and complementary methods for large-scale structural analysis of mammalian chromatin. Genome Res. 17: 928-999, 2007.
Fan, H.-Y., Trotter, K., Archer, T., and Kingston, R. E. : Swapping function of two chromatin remodeling complexes. Molecular Cell 17: 805-815, 2005.
Fan, H.-Y., He, X., Kingston, R. E. and Narlikar, G. J. : Distinct strategies to make nucleosomal DNA accessible. Molecular Cell 11: 1311-1322, 2003.
Yu, A., Fan, H. -Y., Liao, D., Bailey, A. D. and Weiner, A. M. : Activation of p53 or loss of the Cockayne syndrome group B repair protein causes metaphase fragility of human U1, U2 and 5S genes. Molecular Cell 5: 801-810, 2000.