Christopher G. Burd, Ph.D.
Associate Professor of Cell and Developmental Biology
Department: Cell and Developmental Biology
Graduate Group Affiliations
Contact information
1010 BRB II/III
421 Curie Boulevard
Philadelphia, PA 19104-6058
421 Curie Boulevard
Philadelphia, PA 19104-6058
Office: 215-573-5158
Fax: 215-898-9240
Fax: 215-898-9240
Email:
cburd@mail.med.upenn.edu
cburd@mail.med.upenn.edu
Publications
Education:
B.A. (Biology)
Wake Forest University, 1985.
M.S. (Cell and Molecular Biology)
Northern Illinois University, 1987.
Ph.D. (Biochemistry, Molecular Biology and Cell Biology )
Northwestern University, 1994.
B.A. (Biology)
Wake Forest University, 1985.
M.S. (Cell and Molecular Biology)
Northern Illinois University, 1987.
Ph.D. (Biochemistry, Molecular Biology and Cell Biology )
Northwestern University, 1994.
Links
Search PubMed for articles
Cell and Molecular Biology graduate group faculty webpage.
Biochemistry and Molecular Biophysics graduate group faculty webpage.
Permanent linkSearch PubMed for articles
Cell and Molecular Biology graduate group faculty webpage.
Biochemistry and Molecular Biophysics graduate group faculty webpage.
Description of Research Expertise
Research InterestsARF GTPases, Rab GTPases, endocytosis, secretion, phosphatidylinositol kinase signaling.
Key words: protein targeting, Rab GTPase, Arf GTPase, signaling, Golgi apparatus, secretion, endocytosis
Research techniques: molecular genetics, cell biology, biochemistry, and fluorescence microscopy
Description of Research
Our lab is interested in understanding how proteins and lipids are sorted to different organelles within the cell. Investigation of the molecular sorting mechanisms responsible for the biogenesis and maintenance intracellular organelles has been a central problem in cell biology for many years, and research in the field has provided many insights into human disease. We currently focus on two aspects of molecular sorting:
Regulation by phosphoinositides:
Phosphatidylinositol (PtdIns) is a lipid component of all cellular membranes that has important regulatory functions. PtdIns can be phosphorylated by phosphatidylinositol kinases at several different positions on the lipid head group and each phosphorylated form (collectively called phosphoinositides) appears to regulate different cellular pathways, including sorting of proteins in the endosomal pathway, apoptosis, and the cytoskeleton. One major interest of the lab is to identify phosphoinositide-binding proteins and to learn how they link phosphorylation of PtdIns to molecular sorting events.
Regulation by small GTPases of the Rab and ARF family:
Signaling by Ras-related GTPases of the ARF and Rab families regulate biogenesis and trafficking of transport vesicles between different organelles. Cells express a family of ARF and Rab GTPases and each is localized distinctly within the cell. We are trying to understand the molecular events regulated by ARF and Rab signaling and we are also trying figure out different ARFs and Rabs are localized to distinct organelles.
Rotation Projects for 2008-2009
Please discuss with Dr. Burd.
Lab personnel:
Christopher Wood, post-doc
Jingxuan Liu, graduate student
Selected Publications
Strochlic TI. Schmiedekamp BC. Lee J. Katzmann DJ. Burd CG.: Opposing activities of the Snx3-retromer complex and ESCRT proteins mediate regulated cargo sorting at a common endosome. Molecular Biology of the Cell 19(11): 4694-706, Nov 2008.Schmitz, Karl R. Liu, Jingxuan. Li, Shiqing. Setty, Thanuja Gangi. Wood, Christopher S. Burd, Christopher G. Ferguson, Kathryn M.: Golgi localization of glycosyltransferases requires a Vps74p oligomer. Developmental Cell 14(4): 523-34, Apr 2008.
Strochlic, T.I., Setty, T.G., S.A., Sitaram, A., and Burd, C. G.: Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling. J.Cell Biol. 177: 115-125, 2007.
Liu, J., Sitaram, A., and Burd, C. G.: Regulation of endocytosis and degradation of the yeast copper transporter, Ctr1p, by the Rsp5 ubiquitin ligase. Traffic 8: 1375-1384, 2007.
Lee, S.A., Kovacs, J., Stahelin, R.V., Cheever, M.L., Setty, T.G., Burd, C., Cho, W., Overduin, M., and Kutateladze, T.G.: Molecular mechanism of membrane docking by the VAM7P PX domain. J. Biol. Chem. 281: 37091-37101, 2006.
Geng, J., Shin, M. E., Gilbert, P., Collins, R.N., Burd, C.G., : Yeast Rab-GDI Displacement Factor ortholog, Yip3p, forms distinct complexes with the Ypt1p Rab GTPase and the reticulon, Rtn1p. Euk. Cell 4: 1166-1174, 2005.
Lee, S.A., Eyeson, R., Cheever, M.L., Geng, J., Verkhusha, V.V., Burd, C.G., Overduin, M., and Kutateladze, T.G.: Targeting of the FYVE domain to endosomal membranes is regulated by a histidine switch. Proc. Nat’l. Acad. Sci. (USA) 102: 13052-13057, 2005.
Yoshino, Gangi Setty, S.R., Poynton, C., Whiteman, E.L., Saint-Pol, A., Burd, C.G., Johannes, L., Holzbaur, E.L., Koval, M., McCaffery, J.M., and Marks, M.S., : tGolgin-1 (p230, golgin-245) modulates Shiga toxin transport to the Golgi and Golgi motility toward the microtubule organizing centre. J. Cell Sci. 118: 2279-2293, 2005.
Forgacs, G. Yook, S.Y., Janmey, P.A., Jeong, H., and Burd, C.G.: Role of the cytoskeleton in signaling networks. J. Cell Sci 117: 2769-2775, 2004.
Gangi Setty, S.R., Strochlic, T. Tong, A.H.Y., Boone, C., and Burd, C.G.: Golgi targeting of ARF-like GTPase Arl3p requires its N± acetylation and the integral membrane protein Sys1p. Nature Cell Biology 6: 414-419, 2004.