Roberto Bonasio, Ph.D.
3400 Civic Center Boulevard
Philadelphia, PA 19104
Laurea (Biotechnology )
University of Milan, 2000.
Harvard Medical School, 2006.
Description of Research ExpertiseResearch Interests
Molecular mechanisms of epigenetic memory
Keywords: chromatin, noncoding RNAs, epigenetics, Polycomb, ants.
Description of Research
My laboratory studies the molecular mechanisms of epigenetic memory, which are key to a number of biological processes, including embryonic development, cancer, stem cell pluripotency, and brain function.
Epigenetics allows the inheritance of variation (phenotype) without changes in the DNA sequence (genotype). The fact that pluripotent embryonic stem cells, all sharing the same genome, differentiate into hundreds of cell types implies that information about cellular identity and transcriptional states must be stored somewhere within the cell but not in the primary DNA sequence. It has become apparent that this epigenetic information can be encoded in molecular signatures associated with chromatin, the complex of DNA, RNA, and proteins that packages the genome within the eukaryotic nucleus. These signatures include DNA methylation, histone “marks” and variants, higher-order chromatin structures, and chromatin-associated noncoding RNAs (Figure 1).
The latter constitute the focus of our current research. A large fraction of the genome is transcribed into RNAs that, despite lacking protein-coding potential, perform important regulatory functions. Like proteins, RNA molecules can fold into complex tertiary structures with elaborate surfaces and cavities that mediate highly specific molecular interactions and even catalyze biochemical reactions; like DNA, RNA can form Watson–Crick base pairs with other RNAs or with DNA itself (Figure 2). In other words, RNA is fluent in two languages: the elaborate three-dimensional discourse of proteins and the linear genetic code of DNA. Thus, it seems fitting that RNAs may act as a molecular bridge—an epigenetic “translator”—between chromatin-regulating proteins and the genome sequence. Understanding how noncoding RNAs affect the epigenetic states of cells and organisms will provide us with unprecedented access to the regulatory circuitry that makes multicellular life possible.
We and others have discovered that several chromatin-associated protein complexes bind to noncoding RNAs and that these interactions are essential for their proper recruitment and assembly on chromatin, but we have only scratched the surface of the intricate network of protein–RNA interactions in the nucleus and many questions on how noncoding RNAs regulate epigenetic processes at the molecular, cellular, and organismal level remain unanswered.
We approach these fundamental biological questions from both a mechanistic and a systems-level perspective. We combine traditional biochemistry and molecular biology with genome-wide approaches and computational biology and study both conventional systems (mammalian cells) and nonconventional model organisms, such as ants, which offer new, unexplored avenues to study epigenetics (Figure 3).
Kristin Ingvarsdottir, Ph.D. — Postdoctoral Associate
Tim Christopher — Lab Manager
Chongsheng He — Postdoctoral Associate
Janko Gospocic — Postdoctoral Associate
Robert Warneford-Thomson – PhD student (BMB)
Emily Shields – PhD student (GCB)
Selected PublicationsSimola DF, Graham RJ, Brady CM, Enzmann BL, Desplan C, Ray A, Zwiebel LJ, Bonasio R, Reinberg D, Liebig J, Berger SL: Epigenetic (re)programming of caste-specific behavior in the ant Camponotus floridanus. Science 351(6268): aac6633, Jan 2016.
Bonasio, R., Shiekhattar, R.: Regulation of transcription by long noncoding RNAs. Annu Rev Genet 48: 433-55, 2014.
Beck, D. B., Narendra, V., Drury, W. J., 3rd, Casey, R., Jansen, P. W., Yuan, Z. F., Garcia, B. A., Vermeulen, M., Bonasio, R.: In vivo proximity labeling for the detection of protein-protein and protein-RNA interactions. J Proteome Res 13(12): 6135-43, 2014.
Kaneko, S., Son, J., Shen, S. S., Reinberg, D., Bonasio, R.: PRC2 binds active promoters and contacts nascent RNAs in embryonic stem cells. Nat Struct Mol Biol 20(11): 1258-64, 2013.
Bonasio, R., Lecona, E., Narendra, V., Voigt, P., Parisi, F., Kluger, Y., Reinberg, D.: Interactions with RNA direct the Polycomb group protein SCML2 to chromatin where it represses target genes. Elife 3: e02637, 2014.
Kaneko, S.*, Bonasio, R.*, Saldana-Meyer, R., Yoshida, T., Son, J., Nishino, K., Umezawa, A., Reinberg, D.: Interactions between JARID2 and noncoding RNAs regulate PRC2 recruitment to chromatin. Mol Cell 53(2): 290-300, 2014.
Bonasio, R.: Emerging topics in epigenetics: ants, brains, and noncoding RNAs. Ann N Y Acad Sci 1260: 14-23, 2012.
Bonasio, R., Li, Q., Lian, J., Mutti, N. S., Jin, L., Zhao, H., Zhang, P., Wen, P., Xiang, H., Ding, Y., Jin, Z., Shen, S. S., Wang, Z., Wang, W., Wang, J., Berger, S. L., Liebig, J., Zhang, G., Reinberg, D.: Genome-wide and caste-specific DNA methylomes of the ants Camponotus floridanus and Harpegnathos saltator. Curr Biol 22(19): 1755-64, 2012.
Bonasio, R., Zhang, G., Ye, C., Mutti, N. S., Fang, X., Qin, N., Donahue, G., Yang, P., Li, Q., Li, C., Zhang, P., Huang, Z., Berger, S. L., Reinberg, D., Wang, J., Liebig, J.: Genomic comparison of the ants Camponotus floridanus and Harpegnathos saltator. Science 329(5995): 1068-71, 2010.
Bonasio, R., Tu, S., Reinberg, D.: Molecular signals of epigenetic states. Science 330(6004): 612-6, 2010.