Frederic D. Bushman, Ph.D.
Frederic D. Bushman
Professor of Microbiology
Department: Microbiology
Graduate Group Affiliations
Contact information
426 Johnson Pavilion
Department of Microbiology
3610 Hamilton Walk
Philadelphia, PA 19104-6076
Philadelphia, PA 19104
Department of Microbiology
3610 Hamilton Walk
Philadelphia, PA 19104-6076
Philadelphia, PA 19104
Office: (215) 573-8732
Fax: (215) 573-4856
Fax: (215) 573-4856
Email:
bushman@mail.med.upenn.edu
bushman@mail.med.upenn.edu
Publications
Links
Search PubMed for articles
Cell and Molecular Biology graduate group faculty webpage.
Primary Work Website
Search PubMed for articles
Cell and Molecular Biology graduate group faculty webpage.
Primary Work Website
Education:
B.A. (Biology and English)
Amherst College, 1980.
Ph.D. (Cellular and Developmental Biology)
Harvard University, 1988.
Permanent linkB.A. (Biology and English)
Amherst College, 1980.
Ph.D. (Cellular and Developmental Biology)
Harvard University, 1988.
Description of Research Expertise
Research InterestsVirology, HIV, Poxviruses, DNA modifying enzymes, Lateral DNA transfer.
Key words: HIV, integration, integrase, poxvirus, DNA modifying enzyme, virology, variola, RNA interference, interferon.
Description of Research
Research in the laboratory of Dr. Frederic Bushman focuses on understanding the transfer of genetic information between cells and
organisms. Phenomena as diverse as viral replication, microbial pathogenesis, immune system development and genomic evolution are all mediated in large measure by reactions that transfer and rearrange DNA sequences. Research is further focused on DNA modifying enzymes encoded by medically relevant pathogens, since these are a particularly interesting set and also important as drug targets. Major projects in the laboratory focus on two viral model systems: HIV and poxviruses.
Rotation Projects
Rotation projects center on biochemical studies of replication by
retroviruses or poxviruses.
Lab personnel:
Staff Scientists
Young Hwang
Post-doctoral researchers
Troy Brady
Gary Wang
Graduate students
Rithun Mukherjee
Keshet Ronen
Research Assistant
Christian Hoffmann
Nana Minkah
Programmer
Nirav Malani
Scott Sherrill-Mix
Administrative Coordinator
Mali Skotheim
Selected Publications
Wang Gary P, Sherrill-Mix Scott A, Chang Kyong-Mi, Quince Chris, Bushman Frederic D: Hepatitis C virus transmission bottlenecks analyzed by deep sequencing. Journal of virology 84(12): 6218-28, Jun 2010.Wang Gary P, Berry Charles C, Malani Nirav, Leboulch Philippe, Fischer Alain, Hacein-Bey-Abina Salima, Cavazzana-Calvo Marina, Bushman Frederic D: Dynamics of gene-modified progenitor cells analyzed by tracking retroviral integration sites in a human SCID-X1 gene therapy trial. Blood 115(22): 4356-66, Jun 2010.
Caporaso J Gregory, Kuczynski Justin, Stombaugh Jesse, Bittinger Kyle, Bushman Frederic D, Costello Elizabeth K, Fierer Noah, Peña Antonio Gonzalez, Goodrich Julia K, Gordon Jeffrey I, Huttley Gavin A, Kelley Scott T, Knights Dan, Koenig Jeremy E, Ley Ruth E, Lozupone Catherine A, McDonald Daniel, Muegge Brian D, Pirrung Meg, Reeder Jens, Sevinsky Joel R, Turnbaugh Peter J, Walters William A, Widmann Jeremy, Yatsunenko Tanya, Zaneveld Jesse, Knight Rob: QIIME allows analysis of high-throughput community sequencing data. Nature methods 7(5): 335-6, May 2010.
Culyba Matthew J, Hwang Young, Hu Jimmy Yan, Minkah Nana, Ocwieja Karen E, Bushman Frederic D: Metal cofactors in the structure and activity of the fowlpox resolvase. Journal of molecular biology 399(1): 182-95, May 2010.
Gupta Kushol, Diamond Tracy, Hwang Young, Bushman Frederic, Van Duyne Gregory D: Structural properties of HIV integrase-LEDGF oligomers. The Journal of biological chemistry Apr 2010.
Mukherjee Rithun, Plesa Gabriela, Sherrill-Mix Scott, Richardson Max W, Riley James L, Bushman Frederic D: HIV sequence variation associated with env antisense adoptive T-cell therapy in the hNSG mouse model. Molecular therapy : the journal of the American Society of Gene Therapy 18(4): 803-11, Apr 2010.
Gijsbers Rik, Ronen Keshet, Vets Sofie, Malani Nirav, De Rijck Jan, McNeely Melissa, Bushman Frederic D, Debyser Zeger: LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin. Molecular therapy : the journal of the American Society of Gene Therapy 18(3): 552-60, Mar 2010.
Hill D A, Hoffmann C, Abt M C, Du Y, Kobuley D, Kirn T J, Bushman F D, Artis D: Metagenomic analyses reveal antibiotic-induced temporal and spatial changes in intestinal microbiota with associated alterations in immune cell homeostasis. Mucosal immunology 3(2): 148-58, Mar 2010.
Perry Kay, Hwang Young, Bushman Frederic D, Van Duyne Gregory D: Insights from the structure of a smallpox virus topoisomerase-DNA transition state mimic. Structure (London, England : 1993) 18(1): 127-37, Jan 2010.
Caporaso J Gregory, Bittinger Kyle, Bushman Frederic D, DeSantis Todd Z, Andersen Gary L, Knight Rob: PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics (Oxford, England) 26(2): 266-7, Jan 2010.