Toshinori Hoshi, PhD
Toshinori Hoshi, PhD
Professor of Physiology
Department: Physiology
Graduate Group Affiliations
Contact information
D100 Richards
3700 Hamilton Walk
Department of Physiology
Philadelphia, PA 19104
3700 Hamilton Walk
Department of Physiology
Philadelphia, PA 19104
Office: 215-573-7305
Email:
hoshi@mail.med.upenn.edu
hoshi@mail.med.upenn.edu
Publications
Links
Search PubMed for articles
Neuroscience graduate group faculty
The Hoshi Laboratory
Laboratory webpage
Search PubMed for articles
Neuroscience graduate group faculty
The Hoshi Laboratory
Laboratory webpage
Education:
B.A. (Psychology)
University of New Hampshire, 1981.
Ph.D. (Physiology)
Yale University, 1985.
Permanent linkB.A. (Psychology)
University of New Hampshire, 1981.
Ph.D. (Physiology)
Yale University, 1985.
Description of Research Expertise
RESEARCH INTERESTSIon channel modulation such as calcium-dependent potassium channels by heme, heme breakdown products such as CO, and lipid messengers including PIP2 and omega-3 fatty acids
KEY WORDS:
neuronal excitability, ion channel gating, oxidation, methionine, aging, blood, heme, CO, brain hemorrhage, lipids, PIP2, omega-3 fatty acids, fish oil, BK channel, Slo1, KCNMA1, KCNMB, KCa1.1, LRRC, potassium channels, voltage sensor, Shaker channels, EAG, Kv, KCNH
RESEARCH TECHNIQUES
Electrophysiology (patch clamp, voltage-clamp, extracellular recording, optical recording); molecular biology; tissue culture; vascular smooth muscle physiology; physiology and behavior.
RESEARCH SUMMARY
We study modulation of ion channels such as large-conductance calcium- and voltage-gated potassium channels and EAG channels by different signaling molecules. The modulators/modulatory processes being examined include: oxidation/reduction of amino acids, carbon monoxide, heme, bilirubin oxidation end products, PIP2 and omega-3 fatty acids.
Selected Publications
Kolbe Katrin, Schönherr Roland, Gessner Guido, Sahoo Nirakar, Hoshi Toshinori, Heinemann Stefan H: Cysteine 723 in the C-linker segment confers oxidative inhibition of hERG1 potassium channels. The Journal of physiology 588(Pt 16): 2999-3009, Aug 2010.Hou Shangwei, Xu Rong, Clark Joseph F, Wurster William L, Heinemann Stefan H, Hoshi Toshinori: Bilirubin oxidation end products directly alter K(+) channels important in the regulation of vascular tone. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Apr 2010.
Hou Shangwei, Vigeland Leif E, Zhang Guangping, Xu Rong, Li Min, Heinemann Stefan H, Hoshi Toshinori: Zn2+ activates large conductance Ca2+-activated K+ channel via an intracellular domain. The Journal of biological chemistry 285(9): 6434-42, Feb 2010.
Wu Mark N, Joiner William J, Dean Terry, Yue Zhifeng, Smith Corinne J, Chen Dechun, Hoshi Toshinori, Sehgal Amita, Koh Kyunghee: SLEEPLESS, a Ly-6/neurotoxin family member, regulates the levels, localization and activity of Shaker. Nature neuroscience 13(1): 69-75, Jan 2010.
Satz JS, Ostendorf AP, Hou S, Turner A, Kusano H, Lee JC, Turk R, Nguyen H, Ross-Barta SE, Westra S, Hoshi T, Moore SA, Campbell KP.: Distinct functions of glial and neuronal dystroglycan in the developing and adult mouse brain. J Neurosci 30: 14560-14572, 2010.
Hou Shangwei, Horrigan Frank T, Xu Rong, Heinemann Stefan H, Hoshi Toshinori: Comparative effects of H+ and Ca2+ on large-conductance Ca2+- and voltage-gated Slo1 K+ channels. Channels (Austin, Tex.) 3(4): 249-58, Jul-Aug 2009.
Hou Shangwei, Heinemann Stefan H, Hoshi Toshinori: Modulation of BKCa channel gating by endogenous signaling molecules. Physiology (Bethesda, Md.) 24: 26-35, Feb 2009.
Haenold Ronny, Wassef Ramez, Brot Nathan, Neugebauer Sophie, Leipold Enrico, Heinemann Stefan H, Hoshi Toshinori: Protection of vascular smooth muscle cells by over-expressed methionine sulphoxide reductase A: role of intracellular localization and substrate availability. Free radical research 42(11-12): 978-88, Nov 2008.
Horrigan Frank T, Hoshi Toshinori: Integration of an electric-metal sensory experience in the Slo1 BK channel. Nature structural & molecular biology 15(11): 1130-2, Nov 2008.
Kassmann Mario, Hansel Alfred, Leipold Enrico, Birkenbeil Jan, Lu Song-Qing, Hoshi Toshinori, Heinemann Stefan H: Oxidation of multiple methionine residues impairs rapid sodium channel inactivation. Pflügers Archiv : European journal of physiology 456(6): 1085-95, Sep 2008.