Maria P. Limberis, Ph.D.

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Research Associate Professor of Medicine
Department: Medicine

Contact information
125 South 31st street, office 1308, TRL
Philadelphia, PA 19103
Office: 215-898-3123
Fax: 215-494-5444
B.Sc. Hons (Biochemistry)
Liverpool John Moores University, United Kingdom, 1997.
Ph.D. (Molecular Biology)
University of Adelaide, Australia, 2003.
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Description of Research Expertise

Dr. Limberis is an expert in lung-directed gene transfer using viral-based vectors. She is developing AAV-based prophylactic vaccines for deadly airborne infectious diseases. She also investigates gene therapy for cystic fibrosis and studies the immunological implications of lung-directed gene therapy with an emphasis on cellular immune activation to therapeutic transgene products.

Selected Publications

Greig JA, Limberis MP, Bell P, Chen SJ, Calcedo R, Rader DJ, Wilson JM.: Non-Clinical Study Examining AAV8.TBG.hLDLR Vector-Associated Toxicity in Chow-Fed Wild-Type and LDLR+/- Rhesus Macaques. Hum Gene Ther Clin Dev 28(1): 39-50, Mar 2017.

Greig JA, Limberis MP, Bell P, Chen SJ, Calcedo R, Rader DJ, Wilson JM.: Nonclinical Pharmacology/Toxicology Study of AAV8.TBG.mLDLR and AAV8.TBG.hLDLR in a Mouse Model of Homozygous Familial Hypercholesterolemia. Hum Gene Ther Clin Dev 28(1): 28-38, Mar 2017.

Davey MG, Riley JS, Andrews A, Tyminski A, Limberis M, Pogoriler JE, Partridge E, Olive A, Hedrick HL, Flake AW, Peranteau WH.: Induction of Immune Tolerance to Foreign Protein via Adeno-Associated Viral Vector Gene Transfer in Mid-Gestation Fetal Sheep. PLoS One 12(1): 0171132, Jan 2017.

McClain LE, Davey MG, Zoltick PW, Limberis MP, Flake AW, Peranteau WH: Vector serotype screening for use in ovine perinatal lung gene therapy. J Pediatr Surg 51(6): 879-84, 2016.

Alton EW, Armstrong DK, Ashby D, Bayfield KJ, Bilton D, Bloomfield EV, Boyd AC, Brand J, Buchan R, Calcedo R, Carvelli P, Chan M, Cheng SH, Collie DD, Cunningham S, Davidson HE, Davies G, Davies JC, Davies LA, Dewar MH, Doherty A, Donovan J, Dwyer NS, Elgmati HI, Featherstone RF, Gavino J, Gea-Sorli S, Geddes DM, Gibson JS, Gill DR, Greening AP, Griesenbach U, Hansell DM, Harman K, Higgins TE, Hodges SL, Hyde SC, Hyndman L, Innes JA, Jacob J, Jones N, Keogh BF, Limberis MP, Lloyd-Evans P, Maclean AW, Manvell MC, McCormick D, McGovern M, McLachlan G, Meng C, Montero MA, Milligan H, Moyce LJ, Murray GD, Nicholson AG, Osadolor T, Parra-Leiton J, Porteous DJ, Pringle IA, Punch EK, Pytel KM, Quittner AL, Rivellini G, Saunders CJ, Scheule RK, Sheard S, Simmonds NJ, Smith K, Smith SN, Soussi N, Soussi S, Spearing EJ, Stevenson BJ, Sumner-Jones SG, Turkkila M, Ureta RP, Waller MD, Wasowicz MY, Wilson JM, Wolstenholme-Hogg P.: Repeated nebulisation of non-viral CFTR gene therapy in patients with cystic fibrosis: a randomised, double-blind, placebo-controlled, phase 2b trial. Lancet Respir Med 3(9): 684-91, Sep 2015

Limberis M.P., Adam V.S., Wong G., Gren J., Kobasa D., Ross T.M., Kobinger G.P., Tretiakova A., Wilson J.M.: Intranasal antibody gene transfer in mice and ferrets elicits broad protection against pandemic influenza. Science Translational Medicine 5(187): 187ra72, May 2013.

Stitelman D., Brazelton T., Bora A., Traas J., Joyeux L., Merianos D., Limberis M.P., Davey M.G., Flake A.W.: Developmental Stage Determines the Efficiency of Gene Transfer to Muscle Satellite Cells by In Utero Delivery of Adeno-Associated Virus Vector Serotype 2/9. Molecular Therapy - Methods and Clinical Development 1(14040), 2014 Notes: doi:10.1038/mtm.2014.40.

Adam V.S., Crosariol M., Kumar S., Ge M.Q., Czack S.E., Roy S., Haczku A., Tretiakova A., Wilson J.M., Limberis M.P. : Adeno-associated virus 9-mediated airway expression of antibody protects old and immunodeficient mice against influenza virus. Clinical and Vaccine Immunology 21(11): 1528-33, Nov 2014.

Limberis MP, Tretiakova A, Nambiar K, Wong G, Racine T, Crosariol M, Xiangguo Q, Kobinger G, Wilson JM.: Adeno-Associated Virus Serotype 9-Expressed ZMapp in Mice Confers Protection Against Systemic and Airway-Acquired Ebola Virus Infection. J Infect Dis 12(214): 1975-1979, Dec 2016.

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Last updated: 02/14/2018
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