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Changhong Li, MD, Ph.D

Changhong Li, MD, Ph.D

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Research Assistant Professor of Pediatrics
Department: Pediatrics

Contact information
802B, ARC
3516 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
Education:
B.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
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Description of Research Expertise

Research Interest:

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.

Techniques:

Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.

Selected Publications

Qiujing Yua, Bin Zhao, Jun Gui, Kanstantsin V. Katlinski, Angela Brice, Yan Gao, ChangHong Li, Jake A. Kushner, Constantinos Koumenis, J. Alan Diehl, and Serge Y. Fuchs : Type I interferons mediate pancreatic toxicities of PERK inhibition. Proc Natl Acad Sci U S A. 112(50): 15420-15425, Dec 2015.

Dana Avrahami, Changhong Li, Jia Zhang, Jonathan Schug, Ran Avrahami, Shilpa Rao, Michael B. Stadler, Lukas Burger, Dirk Schübeler, Benjamin Glaser and Klaus H.Kaestner. : Aging-dependent demethylation of regulatory elements correlates with chromatin state and improved β-cell function. Cell Metabolism 22(4): 619-32, October 2015.

Nicolai M Doliba, Qing Liu, Changhong Li, Jie Chen, Pan Chen, Chengyang Liu, David W Frederick, Joseph A Baur, Michael J Bennet, Ali Naji, Franz M Matschinsky: Accumulation of 3-hydroxytetradecenoic acid: Cause or corollary of glucolipotoxic impairment of pancreatic β-cell bioenergetics? Molecular Metabolism 4(12): 926-939, October 2015.

Nicolai M. Doliba, Qing Liu, Changhong Li, Pan Chen, Jie Chen, Chengyang Liu, Ali Naji, Michael Bennett, Franz M. Matschinsky: Long-chain beta-hydroxylated fatty acids as mediators of pancreatic beta-cell bioenergetics failure. Diabetes 64(Suppl 1): A597, June 2015.

Ming Li, Pan Chen, Kara E. Boodhansingh, Lixin Wang, Xinhua Xiao, Franz M. Matschinaky, Charles A. Stanley, Diva D. De León, Changhong Li. : Congenital hyperinsulinism due to novel gain of function mutations of glucokinase. Diabetes 64 (Suppl 1): A589, June 2015.

Pan Chen, Ming Li, Diva D. De León, Charles Stanley, Changhong Li. : Impaired glucagon secretion contributes to hypoglycemia phenotype in hyperinsulinism mouse models. Diabetes 64(Suppl 1): A537, June 2015.

Brett Kaufman*, Changhong Li*, Scott A. Soleimanpour. (*:co-first author): Mitochondrial regulation of β-cell function: maintaining the momentum for insulin release. Mol Aspects Med. 42: 91-104, April 2015.

Martha Susiarjo, Frances Xin, Amita Bansal, Martha Stefaniak, Changhong Li, Rebecca A Simmons, Marisa S Bartolomei.: Bisphenol A exposure disrupts metabolic health across multiple generations in the mouse. Endocrinology 156(6): 2014-2027, March 2015.

Changhong Li: Insulin Secretion and the Glutamine-Glutamate-Alpha-Ketoglutarate Axis. Glutamine in Clinical Nutrition. Springer New York, Page: 239-254, Jan. 2015.

Richard G Kibbey, Cheol Soo Choi, Hui-Young Lee, Over Cabrera, Rebecca L Pongratz, Xiaojian Zhao, Andreas L Birkenfeld, Changhong Li, Per-Olof Berggren, Charles Stanley, Gerald I Shulman. : Mitochondrial GTP insensitivity contributes to hypoglycemia in Hyperinsulinemia Hyperammonemia (HI/HA) by inhibiting glucagon release. Diabetes 63: 4218-4229, December 2014.

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Last updated: 12/22/2015
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