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Changhong Li, MD, Ph.D

Changhong Li, MD, Ph.D

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Research Assistant Professor of Pediatrics
Department: Pediatrics

Contact information
802B, ARC
3516 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
B.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
Permanent link
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Description of Research Expertise

Research Interest:

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.


Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.

Selected Publications

Dana Avrahami, Changhong Li, Jia Zhang, Jonathan Schug, Ran Avrahami, Shilpa Rao, Michael B. Stadler, Lukas Burger, Dirk Schübeler, Benjamin Glaser and Klaus H.Kaestner. : Aging-dependent demethylation of regulatory elements correlates with chromatin state and improved β-cell function. Cell Metabolism 22(4): 619-32, October 2015.

Nicolai M Doliba, Qing Liu, Changhong Li, Jie Chen, Pan Chen, Chengyang Liu, David W Frederick, Joseph A Baur, Michael J Bennet, Ali Naji, Franz M Matschinsky: Accumulation of 3-hydroxytetradecenoic acid: Cause or corollary of glucolipotoxic impairment of pancreatic β-cell bioenergetics? Molecular Metabolism October 2015.

Nicolai M. Doliba, Qing Liu, Changhong Li, Pan Chen, Jie Chen, Chengyang Liu, Ali Naji, Michael Bennett, Franz M. Matschinsky: Long-chain beta-hydroxylated fatty acids as mediators of pancreatic beta-cell bioenergetics failure. Diabetes 64(Suppl 1): A597, June 2015.

Ming Li, Pan Chen, Kara E. Boodhansingh, Lixin Wang, Xinhua Xiao, Franz M. Matschinaky, Charles A. Stanley, Diva D. De León, Changhong Li. : Congenital hyperinsulinism due to novel gain of function mutations of glucokinase. Diabetes 64 (Suppl 1): A589, June 2015.

Pan Chen, Ming Li, Diva D. De León, Charles Stanley, Changhong Li. : Impaired glucagon secretion contributes to hypoglycemia phenotype in hyperinsulinism mouse models. Diabetes 64(Suppl 1): A537, June 2015.

Brett Kaufman*, Changhong Li*, Scott A. Soleimanpour. (*:co-first author): Mitochondrial regulation of β-cell function: maintaining the momentum for insulin release. Mol Aspects Med. 42: 91-104, April 2015.

Martha Susiarjo, Frances Xin, Amita Bansal, Martha Stefaniak, Changhong Li, Rebecca A Simmons, Marisa S Bartolomei.: Bisphenol A exposure disrupts metabolic health across multiple generations in the mouse. Endocrinology 156(6): 2014-2027, March 2015.

Changhong Li: Insulin Secretion and the Glutamine-Glutamate-Alpha-Ketoglutarate Axis. Glutamine in Clinical Nutrition. Springer New York, Page: 239-254, Jan. 2015.

Richard G Kibbey, Cheol Soo Choi, Hui-Young Lee, Over Cabrera, Rebecca L Pongratz, Xiaojian Zhao, Andreas L Birkenfeld, Changhong Li, Per-Olof Berggren, Charles Stanley, Gerald I Shulman. : Mitochondrial GTP insensitivity contributes to hypoglycemia in Hyperinsulinemia Hyperammonemia (HI/HA) by inhibiting glucagon release. Diabetes 63: 4218-4229, December 2014.

MING LI, JINHUA YIN, LU XU, SHAN GAO, BCAMS group, CHANGHONG LI: A Large Cohort Study of Chinese Children Suggests Adipokine Profile as New Biomarkers to Define Metabolically Healthy Obesity. Diabetes 63(Suppl 1): A316, June 2014.

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Last updated: 11/10/2015
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