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Changhong Li, MD, Ph.D

Changhong Li, MD, Ph.D

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Research Assistant Professor of Pediatrics
Department: Pediatrics

Contact information
802B, ARC
3516 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
B.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
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Description of Research Expertise

Research Interest:

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.


Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.

Selected Publications

Brett Kaufman*, Changhong Li*, Scott A. Soleimanpour. (*:co-first author): Mitochondrial regulation of β-cell function: maintaining the momentum for insulin release. Mol Aspects Med. 42: 91-104, April 2015.

Martha Susiarjo, Frances Xin, Amita Bansal, Martha Stefaniak, Changhong Li, Rebecca A Simmons, Marisa S Bartolomei.: Bisphenol A exposure disrupts metabolic health across multiple generations in the mouse. Endocrinology Page: online, March 2015.

Changhong Li: Insulin Secretion and the Glutamine-Glutamate-Alpha-Ketoglutarate Axis. Glutamine in Clinical Nutrition. Springer New York, Page: 239-254, 1 2015.

Richard G Kibbey, Cheol Soo Choi, Hui-Young Lee, Over Cabrera, Rebecca L Pongratz, Xiaojian Zhao, Andreas L Birkenfeld, Changhong Li, Per-Olof Berggren, Charles Stanley, Gerald I Shulman. : Mitochondrial GTP insensitivity contributes to hypoglycemia in Hyperinsulinemia Hyperammonemia (HI/HA) by inhibiting glucagon release. Diabetes 63: 4218-4229, December 2014.

Jaeckle Santos LJ, Li C, Doulias PT, Ischiropoulos H, Worthen GS, Simmons RA: Neutralizing Th2 inflammation in neonatal islets prevents β-cell failure in adult IUGR rats. Diabetes 63(5): 1672-84, May 2014.

Itzhak Nissim, Oksana Horyn, Yevgeny Daikhin, Pan Chen, Changhong Li, Suzanne L Wehrli, Ilana Nissim, Marc Yudkoff: The Molecular and Metabolic Influence of Long-Term Agmatine Consumption. J. Biol. Chem. 289: 9710-9729, April 2014.

Yi-Ju Chen, Stacy R. Finkbeiner, Daniel Weinblatt, Matthew J. Emmett, Feven Tameire, Maryam Yousefi, Chenghua Yang, Rene Maehr, Qiao Zhou, Ruth Shemer, Yuval Dor, Changhong Li, Jason R. Spence, and Ben Z. Stanger: De Novo Formation of Insulin-Producing ‘‘Neo-b Cell Islets’’ from Intestinal Crypts. Cell Reports 6: 1-13, March 2014.

Tao Gao, Brian McKenna, Changhong Li, Maximilian Reichert, James Nguyen, Tarjinder Singh, Chenghua Yang, Archana Pannikar, Nicolai Doliba, Tingting Zhang, Doris A. Stoffers, Helena Edlund, Franz Matschinsky, Roland Stein, and Ben Z. Stanger: Pdx1 maintains β-cell identity and function by repressing an alpha-cell program. Cell Metabolism. 19: 259-271, Feb 2014.

Dana Avrahami, Changhong Li, Ming Yu, Yang Jiao, Jia Zhang, Ali Naji, Seyed Ziaie, Benjamin Glaser, Klaus H. Kaestner. : Targeting the cell cycle inhibitor p57Kip2 promotes adult human β cell replication. J Clin Invest. Jan, 16 2014.

Daniel J. Sartori, Christopher J. Wilbur, Simon Y. Long, Matthew M. Rankin, Changhong Li, Jonathan P. Bradfield, Hakon Hakonarson, Struan F. A. Grant, William T. Pu and Jake A. Kushner: GATA Factors Promote ER Integrity and β-Cell Survival and Contribute to Type 1 Diabetes Risk. Mol Endocrinol. 28(1): 28-39, 2014.

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Last updated: 04/29/2015
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