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Changhong Li, MD, Ph.D

Changhong Li, MD, Ph.D

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Research Assistant Professor of Pediatrics
Department: Pediatrics

Contact information
802B, ARC
3516 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
Education:
B.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
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Description of Research Expertise

Research Interest:

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.

Techniques:

Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.

Selected Publications

Jaeckle Santos LJ, Li C, Doulias PT, Ischiropoulos H, Worthen GS, Simmons RA: Neutralizing Th2 inflammation in neonatal islets prevents β-cell failure in adult IUGR rats. Diabetes 63(5): 1672-84, May 2014.

Yi-Ju Chen, Stacy R. Finkbeiner, Daniel Weinblatt, Matthew J. Emmett, Feven Tameire, Maryam Yousefi, Chenghua Yang, Rene Maehr, Qiao Zhou, Ruth Shemer, Yuval Dor, Changhong Li, Jason R. Spence, and Ben Z. Stanger: De Novo Formation of Insulin-Producing ‘‘Neo-b Cell Islets’’ from Intestinal Crypts. Cell Reports 6: 1-13, March 2014.

Tao Gao, Brian McKenna, Changhong Li, Maximilian Reichert, James Nguyen, Tarjinder Singh, Chenghua Yang, Archana Pannikar, Nicolai Doliba, Tingting Zhang, Doris A. Stoffers, Helena Edlund, Franz Matschinsky, Roland Stein, and Ben Z. Stanger: Pdx1 maintains β-cell identity and function by repressing an alpha-cell program. Cell Metabolism. 19: 259-271, Feb 2014.

Dana Avrahami, Changhong Li, Ming Yu, Yang Jiao, Jia Zhang, Ali Naji, Seyed Ziaie, Benjamin Glaser, Klaus H. Kaestner. : Targeting the cell cycle inhibitor p57Kip2 promotes adult human β cell replication. J Clin Invest. Jan, 16 2014.

Daniel J. Sartori, Christopher J. Wilbur, Simon Y. Long, Matthew M. Rankin, Changhong Li, Jonathan P. Bradfield, Hakon Hakonarson, Struan F. A. Grant, William T. Pu and Jake A. Kushner: GATA Factors Promote ER Integrity and β-Cell Survival and Contribute to Type 1 Diabetes Risk. Mol Endocrinol. 28(1): 28-39, 2014.

Itzhak Nissim, Oksana Horyn, Yevgeny Daikhin, Pan Chen, Changhong Li, Suzanne L Wehrli, Ilana Nissim, Marc Yudkoff: The Molecular and Metabolic Influence of Long-Term Agmatine Consumption. J. Biol. Chem. Page: online, 2 2014.

Ming Li, Changhong Li, Aron Allen, Charles A. Stanley, Thomas J. Smith: Glutamate Dehydrogenase: Structure, Allosteric Regulation, and Role in Insulin Homeostasis. Neurochem Res Oct, 12 2013.

Changhong Li, Chengyang Liu, Itzhak Nissim, Jie Chen, Pan Chen, Nicolai Doliba, Ilina Nissim, Yevgeny Daikhin, David Stokes, Marc Yudkoff, Michael J. Bennett, Charles A. Stanley, Franz M. Matschinsky, Ali Naji. : Regulation of Glucagon Secretion in Normal and Diabetic Human Islets by Gamma-Hydroxybutyrate and Glycine. J Biol Chem. 288(8): 3938-51, Feb 2013.

Tingting Zhang, Changhong Li. : Mechanisms of amino acid-stimulated insulin secretion in congenital hyperinsulinism. Acta Biochim Biophys Sin (Shanghai). 45(1): 36-43, Jan 2013.

Ashley Warnock, Lu Tan, Changhong Li, Kristina an Haack, Srinivas B. Narayan, Michael J. Bennett: Amlodipine prevents apoptotic cell death by correction of elevated intracellular calcium in a primary neuronal model of Batten disease (CLN3 disease). Biochem Biophys Res Commun. 436: 645-9, 4 2013.

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Last updated: 07/18/2014
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