Changhong Li, MD, Ph.D
Changhong Li, MD, Ph.D
Research Assistant Professor of Pediatrics
Department: Pediatrics
Contact information
802B, ARC
3516 Civic Center Blvd.
Philadelphia, PA 19104
3516 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
Fax: (215)5901605
Email:
li@email.chop.edu
li@email.chop.edu
Education:
B.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
Permanent linkB.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
Description of Research Expertise
Research Interest:My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.
Techniques:
Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.
Selected Publications
Changhong Li, Chengyang Liu, Itzhak Nissim, Jie Chen, Pan Chen, Nicolai Doliba, Ilina Nissim, Yevgeny Daikhin, David Stokes, Marc Yudkoff, Michael J. Bennett, Charles A. Stanley, Franz M. Matschinsky, Ali Naji. : Regulation of Glucagon Secretion in Normal and Diabetic Human Islets by Gamma-Hydroxybutyrate and Glycine. J Biol Chem. 288(8): 3938-51, Feb 2013.Tingting Zhang, Changhong Li. : Mechanisms of amino acid-stimulated insulin secretion in congenital hyperinsulinism. Acta Biochim Biophys Sin (Shanghai). 45(1): 36-43, Jan 2013.
Yan Gao, Daniel J. Sartori, Changhong Li, Qian-Chun Yu, Jake A. Kushner, Celeste M. Simon, J. Alan Diehl. : PERK is required in the adult pancreas and is essential for maintenance of glucose homeostasis. Mol Cell Biol. 32 (24): 5129-5139, Dec 2012.
Lori S. Hart, John T. Cunningham, Tatini Datta, Souvik Dey, Feven Tameire, Stacey L. Lehman, Bo Qiu, Haiyan Zhang, George Cerniglia, Meixia Bi, Yan Li, Yan Gao, Huayi Liu, Changhong Li, Amit Maity, Andrei Thomas-Tikhonenko, Alexander E. Perl, Albert Koong, Serge Y. Fuchs, J. Alan Diehl, Ian G. Mills, Davide Ruggero, and Constantinos Koumenis.: Endoplasmic Reticulum Stress-Mediated Autophagy Promotes Myc-Dependent Transformation and Tumor Growth. J Clin Invest. 122(12): 4621-34, Nov 2012.
Andrew C Calabria, Changhong Li, Paul R Gallagher, Charles A Stanley, Diva D De León. : GLP-1 Receptor Antagonist Exendin-(9-39) Elevates Fasting Blood Glucose Levels in Congenital Hyperinsulinism Owing to Inactivating Mutations in the ATP-Sensitive K+ Channel. Diabetes. 61(10): 2585-91, Oct 2012.
Changhong Li, Franz M. Matschinsky, Charles Stanley: Amino acid stimulated insulin secretion: the role of glutamine-glutamate-a-ketoglutarate axis. Frontiers in Diabetes: Monogenic Hyperinsulinemic Hypoglycemia Disorders. C.A. Stanley D.D. De Leon (eds.). Karger, 21: 112-124, 2012.
Srinivas B Narayan, Stephen R Master, Anthony N Sireci, Charlene Bierl, Paige E Stanley, Changhong Li, Charles A Stanley, Michael J Bennett.: Short-Chain 3-Hydroxyacyl-Coenzyme A Dehydrogenase Associates with a Protein Super-Complex Integrating Multiple Metabolic Pathways. PLoS ONE 7(4): e35048, 2012.
Li C, Li M, Chen P, Narayan S, Matschinsky FM, Bennett MJ, Stanley CA, Smith TJ.: Green tea polyphenols control dysregulated glutamate dehydrogenase in transgenic mice by hijacking the ADP activation site. J Biol Chem. 286(39), Sep 2011.
Doliba NM, Qin W, Najafi H, Liu C, Buettger CW, Sotiris J, Weik-Collins H, Li C Dr, Stanley CA, Wilson DF, Grimsby J, Sarabu R, Naji A, Matschinsky FM.: Glucokinase Activation Repairs Defective Bioenergetics of Islets of Langerhans isolated from Type-2-Diabetics. Am J Physiol Endocrinol Metab Sep 27. 2011.
Li M, Li C, Allen A, Stanley CA, Smith TJ.: The structure and allosteric regulation of glutamate dehydrogenase. Neurochem Int. 59(4): 445-55. 2011.