Changhong Li, MD, Ph.D

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Research Assistant Professor of Pediatrics
Department: Pediatrics

Contact information
The Children's Hospital of Philadelphia
3516 Civic Center Blvd.
802B, ARC
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
Education:
B.A. (Medicine)
Chengde Medical College, Chengde, China, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, Beijing, China, 1996.
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Description of Research Expertise

Research Interest:

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.

Techniques:

Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.

Selected Publications

Shekhar Srivastava, Zhai Li, Irfana Soomro, Ying Sun, Jianhui Wang, Li Bao, William A. Coetzee, Charles A. Stanley, Chonghong Li and Edward Y. Skolnik: Regulation of KATP Channel Trafficking in Pancreatic β Cells by Protein Histidine Phosphorylation. Diabetes 67(5): 849-860, May 2018.

Lei Chen, Junqing Zhang, Changhong Li, Ziwei Wang; Jingjing Li, Dan Zhao, Suxia Wang, Hong Zhang, Youyuan Huang, Xiaohui Guo. : Glycine Transporter-1 and Glycine Receptor Mediate the Antioxidant Effect of Glycine in Diabetic Rat Islets and INS-1 Cells. Free Radical Biology & Medicine Page: doi: 10.1016, May 2018 Notes: epub ahead of print.

Cetewayo S. Rashid, Yu-Chin Lien, Amita Bansal, Lane J. Jaeckle-Santos, Changhong Li, Kyoung-Jae Won, and Rebecca A. Simmons : Transcriptomic analysis reveals novel mechanisms mediating islet dysfunction in the intrauterine growth restricted (IUGR) rat. Endocrinology 159(2): 1035-1049, Feb 2018.

Ming Lu, Changhong Li. : Nutrient sensing in pancreatic islets: lessons from congenital hyperinsulinism and monogenic diabetes. Annals of the New York Academy of Sciences 1411(1): 65-82, Jan 2018.

Christopher E. Gibson, Kara E. Boodhansingh, Changhong Li, Laura Conlin, Pan Chen, Vaneeta Bamba, Diva D. DeLeon, Arupa Ganguly, Charles A. Stanley. : Increased frequency of congenital hyperinsulinism in girls with Turner Syndrome: Possible association with KDM6A haploinsufficiency. Hormone Research in Pediatrics 2018 Notes: accepted for publication.

Mary Barrosse‐Antle, Chang Su, Pan Chen, Kara E Boodhansingh, Thomas J Smith, Charles A Stanley, Diva D De León, Changhong Li: A severe case of hyperinsulinism due to hemizygous activating mutation of glutamate dehydrogenase. Pediatric Diabetes 18(8): 911-916, December 2017.

Shan He,Yongnian Liu, Lijun Meng, Hongxing Sun, Ying Wang, Yun Ji, Janaki Purushe, Pan Chen, Changhong Li, Jozef Madzo, Jean-Pierre Issa, Jonathan Soboloff, Ran Reshef, Bethany Moore, Luca Gattinoni, Yi Zhang. : Ezh2 phosphorylation state determines its capacity to maintain CD8+ T memory precursors for antitumor immunity. Nat Commun. 8(1): 2125:1-14, December 2017.

Ge Li, Linxin Xu, Yanglu Zhao, Lujiao Li, Junling Fu, Qian Zhang, Naishi Li, Xinhua Xiao, Changhong Li, Jie Mi, Shan Gao, Ming Li. : Leptin-adiponectin imbalance as a marker of metabolic syndrome among Chinese children and adolescents: The BCAMS study. PLoS ONE 12(10): e0186222, October 2017.

Nicolai M. Doliba, Qin Liu, Changhong Li, Chengyang Liu, Ali Naji, Franz M. Matschinsky: Inhibition of Cholinergic Potentiation of Insulin Secretion from Pancreatic Islets by Chronic Elevation of Glucose and Fatty Acids: Protection by Casein Kinase 2 Inhibitor. Molecular Metabolism 6(10): 1240-1253, October 2017.

Hong Q. Smith, Changhong Li, Charles A. Stanley, Thomas James Smith: Glutamate Dehydrogenase, a Complex Enzyme at a Crucial Metabolic Branch Point. Neurochem Res. Page: doi: 10.1007, October 2017 Notes: epub ahead of print.

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Last updated: 06/04/2018
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