Tube development and maintenance in C. elegans
C. elegans, signaling, genetics, cell biology, tube development.
Description of Research
Organs are made up of tubes with different sizes and shapes that are specialized for their particular functions. The tiniest tubes, such as many mammalian capillaries, are unicellular, with the lumen actually inside the cell. More than half of all capillaries in the brain and in the renal glomeruli are unicellular tubes. Capillary defects are associated with cardiovascular diseases, stroke and age-associated dementia, but little is known about how narrow capillaries are formed or protected.
My lab's research utilizes the “excretory” or renal-like system of the nematode worm Caenorhabditis elegans as a model system for studying the mechanisms that build, shape and stabilize narrow tubes. C. elegans has many advantages for such studies, including a very simple and well described anatomy that can be visualized by live imaging and that allows phenotypic analysis at single-cell resolution. C. elegans also is highly amenable to powerful forward and reverse genetic approaches to find genes involved in a process of interest. Using forward genetic screens, our lab has identified many genes that are important for building, shaping and stabilizing narrow tubes, and we are studying the pathways and mechanisms through which these act. Some of the questions we're addressing are:
How does auto-fusion promote seamless tube growth and shaping?
We showed that EGF-Ras-ERK signaling controls many aspects of unicellular tube morphology through a key target, the transmembrane fusogen AFF-1. AFF-1 fuses plasma membranes to convert an autocellular "seamed" tube into a "seamless" tube that lacks adherens junctions or tight junctions along its length. We are studying this role of AFF-1 and other cytoskeletal changes and vesicle trafficking pathways that allow seamless tubes to adopt very complex, elongated shapes.
How does the luminal extracellular matrix shape and protect narrow tubes? What is the lipid connection?
Most tubes secrete various glycoproteins into their developing lumens, and there is a growing appreciation of the importance of this luminal matrix in development and disease. We've identified several types of apically localized matrix proteins or lipophilic cargo binding proteins that are required to shape and protect the narrow duct and pore tubes, and to prevent them from bursting or leaking. We've also identified suppressor mutations that "fix" these tube problems. Current studies are examining links among lipids, luminal matrix organization, and the cytoskeleton, and taking advantage of C. elegans' unique advantages to visualize the luminal matrix and understand its assembly and disassembly.
What controls tube delamination and trans-differentiation?
The excretory system is also an excellent model for studying junction remodeling and epithelial fate plasticity. At a specific stage of development, the excretory pore tube delaminates from the organ, loses epithelial identity, re-enters the cell-cycle and generates two neuronal daughters. The lab has identified mutants that perturb delamination, which should provide insight into mechanisms that trigger identity change and allow junction remodeling and delamination.
Jennifer Cohen (graduate student, 2015-present)
Emily Pu (postdoctoral fellow, 2012-present)
Fabien Soulavie (postdoctoral fellow, 2013-present)
Rachel Forman-Rubinsky (research specialist, 2014-present)
Selam Bekele (undergraduate assistant, 2014-present)
Robyn Howard-Barfield (1999-2004), now Group Leader at Catalent Pharma
Craig Stone (2003-2008), now Medical writer at EBSCO
Kelly Howell (2004-2010), now Scientist at SMA Foundation
Vincent Mancuso (2006-2011), now homemaker
Ishmail Abdus-Saboor (2007-2012), now postdoc at UPenn (soon-to-be Assistant Professor at Swarthmore College)
Ranjana Kishore (1998-2002), now Staff Scientist at Caltech
Gautam Kao (1999-2003), now Researcher at Gothenburg U. (Sweden)
Kyunghee Koh (2001-2003), now faculty at Jefferson U.
Chris Rocheleau (2000-2005), now faculty at McGill U.
David Raizen (2001-2007), now faculty at UPenn
Olena Vatamaniuk (2004-2005), now faculty at Cornell U.
Jean Parry (2010-2014), now faculty at Georgian U.
Hasreet K. Gill*, Jennifer D. Cohen*, Jesus Ayala-Figueroa, Rachel Forman-Rubinsky, Corey Poggioli, Kevin Bickard, Jean M. Parry, Pu Pu, David H. Hall and Meera V. Sundaram : Integrity of narrow epithelial tubes in the C. elegans excretory system requires a transient luminal matrix PLoS Genetics 12(8): e1006205, August 2016.
Meera V. Sundaram and Matthew Buechner: The Caenorhabditis elegans excretory system: a model for tubulogenesis, cell fate specification and plasticity. Genetics 203: 35-63, May 2016.
Meera V. Sundaram and Jennifer D. Cohen: Time to make the doughnuts: Building and shaping seamless tubes. Seminars in Cell and Developmental Biology in press, 2017.
Fabien Soulavie and Meera V. Sundaram: Auto-fusion and the shaping of neurons and tubes. Seminars in Cell and Developmental Biology 60: 136-145, December 2016.
Parry, J. M. and Sundaram, M. V.: A cell non-autonomous role for Ras signaling in C. elegans neuroblast delamination. Development 141: 4279-4284, Nov 2014.
Mancuso, V.P., Parry, J. M., Storer, L., Poggioli, C., Nguyen, K. C. Q., Hall, D.H. and Sundaram, M.V: Extracellular leucine-rich repeat proteins are required to organize the apical extracellular matrix and maintain epithelial junction integrity in C. elegans. Development 139: 979-990, March 2012.
Abdus-Saboor, I., Mancuso, V.P., Murray, J.I., Palozola, K., Norris, C., Hall, D.H., Howell, K., Huang, K. and Sundaram, M.V: Notch and Ras promote sequential steps of excretory tube development in C. elegans. Development 138: 3545-3555, August 2011.
Stone, C. E., Hall, D. H., and Sundaram, M. V.: Lipocalin signaling controls unicellular tube development in the Caenorhabditis elegans excretory system. Developmental Biology 329: 201-211, 2009.
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Last updated: 01/09/2017
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