Department of Otorhinolaryngology

Otorhinolaryngology
faculty photo

Denis F Kinane, BDS, FDS RCPS, FDS RCS, PhD

Professor of Otorhinolaryngology: Head and Neck Surgery
Morton Amsterdam Dean, University of Pennsylvania School of Medicine
Department: Otorhinolaryngology: Head and Neck Surgery

Contact information
School of Dental Medicine
Robert Schattner Center
240 S 40th Street, F1G
Philadelphia, PA 19104
Office: 215-898-1038
Lab: 215-746-4189
Education:
BDS
University of Edinburgh, 1980.
PhD (Microbiology)
University of Edinburgh, 1983.
FDS RCS
University of Edinburgh, 1986.
FDS RCPS
University of Glasgow, 1993.
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Description of Research Expertise

Currently we utilize multiple human gingival epithelial cell lines to assess epithelial cell TLR mediated responses to cytokine and bacterial challenges and assess cytokine protein, mRNS for a variety of receptors and secreted proteins. We examine responses by high-throughput microarrays, real time PCR and proteomics and are interested in looking at responses to exposures and inter-individual variations. We have discovered differences in human TLRs which may relate to disease susceptibility, using microarrays, real time PCRs, miRNA arrays, plasmid reporter constructs, phosphorylation assays, multiplex ELISA, confocal and imaging techniques. We have set up a CDC funded initiative (PRSM) to enable real time access to 'omics' developments in inflammation and immunity relevant to periodontal disease. We have recently identified miRNA controlling the protein expression of TLR-2 and several miRNAs specific to apoptosis in human gingival epithelial cells. We have also worked out a novel cellular signalling pathway for antimicrobial peptide human beta defensin-2 and a target molecule for increasing the host antimicrobial peptide to fight against infectious bacteria. We are undertaking real-time monitoring of cells using live cell imaging technique and detailed proteomic analyses of host-microbe interaction with live micro-organisms and pruified ligands to TLRs to determine the possible targets for drug discovery.

Selected Publications

Kulkarni Cyelee, Kinane Denis F: Host response in aggressive periodontitis. Periodontology 2000 65(1): 79-91, Jun 2014.

Lappin David F, Apatzidou Danae, Quirke Anne-Marie, Oliver-Bell Jessica, Butcher John P, Kinane Denis F, Riggio Marcello P, Venables Patrick, McInnes Iain B, Culshaw Shauna: Influence of periodontal disease, Porphyromonas gingivalis and cigarette smoking on systemic anti-citrullinated peptide antibody titres. Journal of clinical periodontology 40(10): 907-15, Oct 2013.

Bıyıkoğlu Başak, Buduneli Nurcan, Aksu Kenan, Nalbantsoy Ayşe, Lappin David F, Evrenosoğlu Evren, Kinane Denis F: Periodontal therapy in chronic periodontitis lowers gingival crevicular fluid interleukin-1beta and DAS28 in rheumatoid arthritis patients. Rheumatology international 33(10): 2607-16, Oct 2013.

Li Shuai, Dong Guangyu, Moschidis Anastasios, Ortiz Javier, Benakanakere Manjunatha R, Kinane Denis F, Graves Dana T: P. gingivalis modulates keratinocytes through FOXO transcription factors. PloS one 8(11): e78541, 2013.

Kinane James A, Benakanakere Manjunatha R, Zhao Jiawei, Hosur Kavita B, Kinane Denis F: Porphyromonas gingivalis influences actin degradation within epithelial cells during invasion and apoptosis. Cellular microbiology 14(7): 1085-96, Jul 2012.

Scott Ann E, Milward Mike, Linden Gerard J, Matthews John B, Carlile Monica J, Lundy Fionnuala T, Naeeni Mojgan A, Lorraine Martin S, Walker Brian, Kinane Denis, Brock Gareth R, Chapple Iain L C: Mapping biological to clinical phenotypes during the development (21 days) and resolution (21 days) of experimental gingivitis. Journal of clinical periodontology 39(2): 123-31, Feb 2012.

Özçaka, Ö., Başoǧlu, Ö.K., Buduneli, N., Taşbakan, M.S., Bacakoǧlu, F., Kinane, D.F. : Chlorhexidine decreases the risk of ventilator-associated pneumonia in intensive care unit patients: A randomized clinical trial Journal of Periodontal Research 47(5): 584-592, 2012.

Benakanakere Manju, Kinane Denis F: Innate cellular responses to the periodontal biofilm. Frontiers of oral biology 15: 41-55, 2012.

Kinane, D.F., Mombelli, A. : Periodontal disease. Foreword. Frontiers of oral biology 15: v-ix, 2012.

Kinane Denis F, Preshaw Philip M, Loos Bruno G: Host-response: understanding the cellular and molecular mechanisms of host-microbial interactions--consensus of the Seventh European Workshop on Periodontology. Journal of clinical periodontology 38 Suppl 11: 44-8, Mar 2011.

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Last updated: 03/29/2013
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