Dr. Weissman's laboratory focuses on the study of RNA and innate immune system biology and the application of these findings to vaccine research and gene therapy. There are three main projects in his laboratory. The first project began through the use of mRNA encoding antigen as a delivery system to load dendritic cells to promote broad immune responses as part of a vaccine. This project has expanded to include basic studies of RNA immunogenicity and translation and the development of applications for gene therapy. The second project develops new HIV envelope immunogens that can induce broad responses and cross-reactive neutralizing antibodies. The third project continues previous studies that identified a protein found on DC, macrophages, and epithelial cells that binds HIV envelope with high affinity. The main focus of this project is testing whether this and related molecules function in vivo to promote HIV genital tract infection.
Ravimohan S, Tamuhla N, Steenhoff AP, Letlhogile R, Makutu DK, Nfanyana K, Rantleru T, Tierney A, Nkakana K, Schwartz AB, Gross R, Macgregor RR, Bellamy SL, Frank I, Weissman D, Bisson GP: Early immunologic failure is associated with early mortality among advanced HIV-infected adults initiating antiretroviral therapy with active tuberculosis. The Journal of Infectious Diseases 208(11): 1784-93, Dec 2013.
Boros G, Miko E, Muramatsu H, Weissman D, Emri E, Rózsa D, Nagy G, Juhász A, Juhász I, van der Horst G, Horkay I, Remenyik E, Karikó K, Emri G: Transfection of pseudouridine-modified mRNA encoding CPD-photolyase leads to repair of DNA damage in human keratinocytes: A new approach with future therapeutic potential. Journal of Photochemistry and Photobiology 129: 93-9, Dec 2013.
Barber C, Weissman D, Barnhart K, Dalvi M, Abrams WR, Malamud D: An electrochemiluminescence assay for gp340 (DMBT1). Analytical Biochemistry 440(1): 78-80, Sep 2013.
Koenig HC, Garland JM, Weissman D, Mounzer K: Vaccinating HIV patients: focus on human papillomavirus and herpes zoster vaccines. AIDS reviews 15(2): 77-86, Apr-Jun 2013.
Bisson GP, Mooketsi M, Thaker R, Steenhoff A, Tsimako I, Tamuhla N, Gluckman S, Ravimohan S, Weissman D, Tebas P: Early versus delayed antiretroviral therapy and CSF fungal clearance in adults with HIV and cryptococcal meningitis. Clinical Infectious Diseases 56(8): 1165-73, Apr 2013.
Weissman D, Pardi N, Muramatsu H, Karikó K: HPLC purification of in vitro transcribed long RNA. Methods in Molecular Biology 969: 43-54, 2013.
Pardi N, Muramatsu H, Weissman D, Karikó K: In vitro transcription of long RNA containing modified nucleosides. Methods in Molecular Biology 969: 29-42, 2013.
Bisson GP, Mehaffy C, Broeckling C, Prenni J, Rifat D, Lun DS, Burgos M, Weissman D, Karakousis PC, Dobos K: Upregulation of the Phthiocerol Dimycocerosate Biosynthetic Pathway by Rifampin-Resistant, rpoB Mutant Mycobacterium tuberculosis. Journal of Bacteriology 194(23): 6441-52, Dec 2012.
Anderson BR, Kariko K, Weissman D: Nucleofection induces transient eIF2a phosphorylation by GCN2 and PERK. Gene Therapy Page: 1-7, February 2012.
Karikó K, Muramatsu H, Keller JM, Weissman D: Increased Erythropoiesis in Mice Injected With Submicrogram Quantities of Pseudouridine-containing mRNA Encoding Erythropoietin. Molecular therapy : the Journal of the American Society of Gene Therapy Feb 2012.
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Last updated: 03/28/2014
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