Untitled Document

Narayan G Avadhani

Graduate Group Affiliations

Pharmacology

Contact information

3800 Spruce Street
Room 189E Vet
Philadelphia, PA 19104

Office: 215-898-8819
Fax: 215-573-6651

Education:
B.Sc. (Chemistry/Physics)
Karnatak Science College, 1962.
Ph.D. (Biochemistry/Genetics)
University of Bombay, 1969.

Post-Graduate Training
Research Associate, University of Illinois, 1969-1970.
Research Assistant Professor, University of Illinois, 1970-1972.
Research Associate, University of Pennsylvania School of Veterinary Medicine, 1972-1973.

Permanent link

Description of Research Expertise

The research in Dr. Avadhani's laboratory is focused on the following aspects of mitochondrial genetics and regulation of mitochondrial membrane biogenesis in mammalian cells:

1. Mechanisms of dual targeting of cytochrome P450 and related proteins to ER and mitochondria and mechanisms of activation of the chimeric N-terminal signal by cAMP and other physiological factors.

2. Characterization of a novel mitochondria-to-nucleus stress signaling in cells subjected to mitochondrial specific genetic, and or, metabolic stress, which operates through altered [Ca2+]c, and the role of mitochondrial stress signaling in tumor progression and metastasis.

3. Regulation of cytochrome oxidase gene expression, and modulation of enzyme assembly/activity under chemical and oxidative stress conditions.

4. Role of mitochondrial stress signaling in Embryonic Stem Cell function/differentiation, and mammalian mitochondrial transcription under chemical and oxidative stress in ES cells.

Selected Publications

Biswas, Gopa. Srinivasan, Satish. Anandatheerthavarada, Hindupur K. Avadhani, Narayan G.: Dioxin-mediated tumor progression through activation of mitochondria-to-nucleus stress signaling. Proceedings of the National Academy of Sciences of the United States of America 105(1): 186-91, Jan 8 2008.

Srinivasan, Satish. Avadhani, Narayan G.: Hypoxia-mediated mitochondrial stress in RAW264.7 cells induces osteoclast-like TRAP-positive cells. Annals of the New York Academy of Sciences 1117: 51-61, Nov 2007.

Sepuri, Naresh B V. Yadav, Sanjay. Anandatheerthavarada, Hindupur K. Avadhani, Narayan G.: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae--role of protein kinase A in the mitochondrial targeting of CYP2E1. FEBS Journal 274(17): 4615-30, Sep 2007.

Hardy, Micael. Rockenbauer, Antal. Vasquez-Vivar, Jeannette. Felix, Christopher. Lopez, Marcos. Srinivasan, Satish. Avadhani, Narayan. Tordo, Paul. Kalyanaraman, B.: Detection, characterization, and decay kinetics of ROS and thiyl adducts of mito-DEPMPO spin trap. Chemical Research in Toxicology 20(7): 1053-60, Jul 2007.

Guha, Manti. Srinivasan, Satish. Biswas, Gopa. Avadhani, Narayan G.: Activation of a novel calcineurin-mediated insulin-like growth factor-1 receptor pathway, altered metabolism, and tumor cell invasion in cells subjected to mitochondrial respiratory stress. Journal of Biological Chemistry 282(19): 14536-46, May 11 2007.

Spear, Joseph F. Prabu, Subbuswamy K. Galati, Domenico. Raza, Haider. Anandatheerthavarada, Hindupur K. Avadhani, Narayan G.: beta1-Adrenoreceptor activation contributes to ischemia-reperfusion damage as well as playing a role in ischemic preconditioning. American Journal of Physiology - Heart & Circulatory Physiology 292(5): H2459-66, May 2007.

Fang, Ji-Kang. Prabu, Subbuswamy K. Sepuri, Naresh B. Raza, Haider. Anandatheerthavarada, Hindupur K. Galati, Domenico. Spear, Joseph. Avadhani, Narayan G.: Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion. FEBS Letters 581(7): 1302-10, Apr 3 2007.

Dasari, Venkata Ramesh. Anandatheerthavarada, Hindupur K. Robin, Marie-Anne. Boopathi, Ettickan. Biswas, Gopa. Fang, Ji-Kang. Nebert, Daniel W. Avadhani, Narayan G.: Role of protein kinase C-mediated protein phosphorylation in mitochondrial translocation of mouse CYP1A1, which contains a non-canonical targeting signal. Journal of Biological Chemistry 281(41): 30834-47, Oct 13 2006.

Wang, Ying. Biswas, Gopa. Prabu, Subbuswamy K. Avadhani, Narayan G.: Modulation of mitochondrial metabolic function by phorbol 12-myristate 13-acetate through increased mitochondrial translocation of protein kinase Calpha in C2C12 myocytes. Biochemical Pharmacology 72(7): 881-92, Sep 28 2006.

Devi, Latha. Prabhu, Badanavalu M. Galati, Domenico F. Avadhani, Narayan G. Anandatheerthavarada, Hindupur K.: Accumulation of amyloid precursor protein in the mitochondrial import channels of human Alzheimer's disease brain is associated with mitochondrial dysfunction. Journal of Neuroscience 26(35): 9057-68, Aug 30 2006.

Prabu, Subbuswamy K. Anandatheerthavarada, Hindupur K. Raza, Haider. Srinivasan, Satish. Spear, Joseph F. Avadhani, Narayan G.: Protein kinase A-mediated phosphorylation modulates cytochrome c oxidase function and augments hypoxia and myocardial ischemia-related injury. Journal of Biological Chemistry 281(4): 2061-70, Jan 27 2006.

Biswas, Gopa. Guha, Manti. Avadhani, Narayan G.: Mitochondria-to-nucleus stress signaling in mammalian cells: nature of nuclear gene targets, transcription regulation, and induced resistance to apoptosis. Gene 354: 132-9, Jul 18 2005.

Biswas, G. Anandatheerthavarada, H K. Avadhani, N G.: Mechanism of mitochondrial stress-induced resistance to apoptosis in mitochondrial DNA-depleted C2C12 myocytes. Cell Death & Differentiation 12(3): 266-78, Mar 2005.

Nikonova, Elena V. Vijayasarathy, Camasamudram. Zhang, Lin. Cater, Jacqueline R. Galante, Raymond J. Ward, Stephen E. Avadhani, Narayan G. Pack, Allan I.: Differences in activity of cytochrome C oxidase in brain between sleep and wakefulness. Sleep 28(1): 21-7, Jan 1 2005.

Boopathi, Ettickan. Lenka, Nibedita. Prabu, Subbuswamy K. Fang, Ji-Kang. Wilkinson, Frank. Atchison, Michael. Giallongo, Agata. Avadhani, Narayan G.: Regulation of murine cytochrome c oxidase Vb gene expression during myogenesis: YY-1 and heterogeneous nuclear ribonucleoprotein D-like protein (JKTBP1) reciprocally regulate transcription activity by physical interaction with the BERF-1/ZBP-89 factor. Journal of Biological Chemistry 279(34): 35242-54, Aug 20 2004.

Butow, Ronald A. Avadhani, Narayan G.: Mitochondrial signaling: the retrograde response. [Review] [119 refs] Molecular Cell 14(1): 1-15, Apr 9 2004.

Levy, Richard J. Vijayasarathy, C. Raj, Nichelle R. Avadhani, Narayan G. Deutschman, Clifford S.: Competitive and noncompetitive inhibition of myocardial cytochrome C oxidase in sepsis. Shock 21(2): 110-4, Feb 2004.