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John W. Weisel, Ph.D.

Professor of Cell and Developmental Biology
Department: Cell and Developmental Biology

Contact information
1154 BRB II/III
421 Curie Boulevard
Philadelphia, PA 19104-6058
Office: 215-898-3573
Fax: 215-898-9871
Education:
B.S. (Electrical Engineering)
Swarthmore College, 1968.
Ph.D. (Biophysics)
Brandeis University, 1973.
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Description of CVI Expertise

CVI Program Unit(s):
Biotechnology / Nanomedicine / Imaging
Thrombosis / Hemostasis

CVI Research Description:
The primary focus of Dr. Weisel's research is the molecular and cellular mechanisms of blood coagulation, fibrinolysis and atherosclerosis, as analyzed through the use of various biochemical and biophysical techniques, including visualization of molecules and supramolecular aggregates and measurements of mechanical properties of cellular and extracellular structures. Structural studies designed to elucidate the intermolecular interactions in all steps of fibrin clot formation and fibrinolysis arebeing carried out. A novel technique using optical trapping has been developed to study protein protein binding/unbinding at the single molecule level. In addition, fibrin polymerization is being studied in real time by confocal and deconvolution microscopy. The determination of relationships between clot structure and mechanical properties is an important part of this work, including investigations into the molecular origins of clot mechanical properties.

Atomic force microscopy is used to characterize the forced unfolding of individual molecules and its role in clot mechanics. The binding of integrins to fibrinogen is also a focus of research, especially in terms of the interactions that are responsible for platelet aggregation. The results of these studies have implications for basic mechanisms of protein-protein and protein-cell interactions as well as for clinical aspects of hemostasis, thrombosis and atherosclerosis.

Selected Publications

Litvinov Rustem I, Weisel John W: Fibrin mechanical properties and their structural origins. Matrix biology : journal of the International Society for Matrix Biology 60-61: 110-123, Jul 2017.

Kononova Olga, Litvinov Rustem I, Blokhin Dmitry S, Klochkov Vladimir V, Weisel John W, Bennett Joel S, Barsegov Valeri: Mechanistic Basis for the Binding of RGD- and AGDV-Peptides to the Platelet Integrin αIIbβ3. Biochemistry 56(13): 1932-1942, Apr 2017.

Silvain Johanne, Collet Jean-Philippe, Guedeney Paul, Varenne Olivier, Nagaswami Chandrasekaran, Maupain Carole, Empana Jean-Philippe, Boulanger Chantal, Tafflet Muriel, Manzo-Silberman Stephane, Kerneis Mathieu, Brugier Delphine, Vignolles Nicolas, Weisel John W, Jouven Xavier, Montalescot Gilles, Spaulding Christian: Thrombus composition in sudden cardiac death from acute myocardial infarction. Resuscitation 113: 108-114, Apr 2017.

Tutwiler Valerie, Wang Hailong, Litvinov Rustem I, Weisel John W, Shenoy Vivek B: Interplay of Platelet Contractility and Elasticity of Fibrin/Erythrocytes in Blood Clot Retraction. Biophysical journal 112(4): 714-723, Feb 2017.

Tutwiler Valerie, Peshkova Alina D, Andrianova Izabella A, Khasanova Dina R, Weisel John W, Litvinov Rustem I: Contraction of Blood Clots Is Impaired in Acute Ischemic Stroke. Arteriosclerosis, thrombosis, and vascular biology 37(2): 271-279, Feb 2017.

Litvinov Rustem I, Weisel John W: Role of red blood cells in haemostasis and thrombosis. ISBT science series 12(1): 176-183, Feb 2017.

Galanakis Dennis K, Spitzer Eric D, Perrotta Peter, Nagaswami Chandrasekaran, Marchi Rita, Spitzer Silvia, Refailovich Miriam, Smith Roy E, Zhang Liudi, Marmorat Clement, Weisel John W: Thrombosis-associated antifibrinogen IgG1 κ impairs fibrin polymerization and enhances platelet activation. Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 28(1): 40-49, Jan 2017.

Weisel John W, Litvinov Rustem I: Fibrin Formation, Structure and Properties. Sub-cellular biochemistry 82: 405-456, 2017.

Cuker Adam, Husseinzadeh Holleh, Lebedeva Tatiana, Marturano Joseph E, Massefski Walter, Lowery Thomas J, Lambert Michele P, Abrams Charles S, Weisel John W, Cines Douglas B: Rapid Evaluation of Platelet Function With T2 Magnetic Resonance. American journal of clinical pathology 146(6): 681-693, Dec 2016.

Zhmurov Artem, Protopopova Anna D, Litvinov Rustem I, Zhukov Pavel, Mukhitov Alexander R, Weisel John W, Barsegov Valeri: Structural Basis of Interfacial Flexibility in Fibrin Oligomers. Structure (London, England : 1993) 24(11): 1907-1917, Nov 2016.

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Last updated: 08/27/2014
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