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John W. Weisel, Ph.D.

Professor of Cell and Developmental Biology
Department: Cell and Developmental Biology

Contact information
1154 BRB II/III
421 Curie Boulevard
Philadelphia, PA 19104-6058
Office: 215-898-3573
Fax: 215-898-9871
Education:
B.S. (Electrical Engineering)
Swarthmore College, 1968.
Ph.D. (Biophysics)
Brandeis University, 1973.
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Description of CVI Expertise

CVI Program Unit(s):
Biotechnology / Nanomedicine / Imaging
Thrombosis / Hemostasis

CVI Research Description:
The primary focus of Dr. Weisel's research is the molecular and cellular mechanisms of blood coagulation, fibrinolysis and atherosclerosis, as analyzed through the use of various biochemical and biophysical techniques, including visualization of molecules and supramolecular aggregates and measurements of mechanical properties of cellular and extracellular structures. Structural studies designed to elucidate the intermolecular interactions in all steps of fibrin clot formation and fibrinolysis arebeing carried out. A novel technique using optical trapping has been developed to study protein protein binding/unbinding at the single molecule level. In addition, fibrin polymerization is being studied in real time by confocal and deconvolution microscopy. The determination of relationships between clot structure and mechanical properties is an important part of this work, including investigations into the molecular origins of clot mechanical properties.

Atomic force microscopy is used to characterize the forced unfolding of individual molecules and its role in clot mechanics. The binding of integrins to fibrinogen is also a focus of research, especially in terms of the interactions that are responsible for platelet aggregation. The results of these studies have implications for basic mechanisms of protein-protein and protein-cell interactions as well as for clinical aspects of hemostasis, thrombosis and atherosclerosis.

Selected Publications

Le Minh Giang, Peshkova Alina D, Andrianova Izabella A, Sibgatullin Timur B, Maksudova Adelia N, Weisel John W, Litvinov Rustem I: Impaired contraction of blood clots as a novel prothrombotic mechanism in systemic lupus erythematosus. Clinical science (London, England : 1979) 132(2): 243-254, Jan 2018.

Vostal Jaroslav G, Buehler Paul W, Gelderman Monique P, Alayash Abdu I, Doctor Alan, Zimring James C, Glynn Simone A, Hess John R, Klein Harvey, Acker Jason P, Spinella Philip C, D'Alessandro Angelo, Palsson Bernhard, Raife Thomas J, Busch Michael P, McMahon Timothy J, Intaglietta Marcos, Swartz Harold M, Dubick Michael A, Cardin Sylvain, Patel Rakesh P, Natanson Charles, Weisel John W, Muszynski Jennifer A, Norris Philip J, Ness Paul M: Proceedings of the Food and Drug Administration's public workshop on new red blood cell product regulatory science 2016. Transfusion 58(1): 255-266, Jan 2018.

Xu Shixin, Xu Zhiliang, Kim Oleg V, Litvinov Rustem I, Weisel John W, Alber Mark: Model predictions of deformation, embolization and permeability of partially obstructive blood clots under variable shear flow. Journal of the Royal Society, Interface 14(136), Nov 2017.

Kim Oleg V, Litvinov Rustem I, Alber Mark S, Weisel John W: Quantitative structural mechanobiology of platelet-driven blood clot contraction. Nature communications 8(1): 1274, Nov 2017.

Höök Peter, Litvinov Rustem I, Kim Oleg V, Xu Shixin, Xu Zhiliang, Bennett Joel S, Alber Mark S, Weisel John W: Strong Binding of Platelet Integrin αIIbβ3 to Fibrin Clots: Potential Target to Destabilize Thrombi. Scientific reports 7(1): 13001, Oct 2017.

Protopopova Anna D, Litvinov Rustem I, Galanakis Dennis K, Nagaswami Chandrasekaran, Barinov Nikolay A, Mukhitov Alexander R, Klinov Dmitry V, Weisel John W: Morphometric characterization of fibrinogen's αC regions and their role in fibrin self-assembly and molecular organization. Nanoscale 9(36): 13707-13716, Sep 2017.

Liang Xiaojun, Chernysh Irina, Purohit Prashant K, Weisel John W: Phase transitions during compression and decompression of clots from platelet-poor plasma, platelet-rich plasma and whole blood. Acta biomaterialia 60: 275-290, Sep 2017.

Bannish Brittany E, Chernysh Irina N, Keener James P, Fogelson Aaron L, Weisel John W: Molecular and Physical Mechanisms of Fibrinolysis and Thrombolysis from Mathematical Modeling and Experiments. Scientific reports 7(1): 6914, Aug 2017.

Höök Peter, Brito-Robinson Teresa, Kim Oleg, Narciso Cody, Goodson Holly V, Weisel John W, Alber Mark S, Zartman Jeremiah J: Whole blood clot optical clearing for nondestructive 3D imaging and quantitative analysis. Biomedical optics express 8(8): 3671-3686, Aug 2017.

Litvinov Rustem I, Weisel John W: Fibrin mechanical properties and their structural origins. Matrix biology : journal of the International Society for Matrix Biology 60-61: 110-123, Jul 2017.

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Last updated: 12/04/2017
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