Michael Ostap

faculty photo
Professor of Physiology
Director, Pennsylvania Muscle Institute
Department: Physiology

Contact information
Pennsylvania Muscle Institute &
Department of Physiology
700A Clinical Research Building
415 Curie Blvd
Philadelphia, PA 19104
Office: (215) 573-9758
Lab: (215) 898-3685
Education:
B.S. (Chemistry)
Illinois State University, 1988.
Ph.D. (Biochemistry, Molecular Biology, and Biophysics)
University of Minnesota, 1993.
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Description of Research Expertise

Research Interests
Molecular Motors, Cell Motility, Mechanosensing, Single-Molecule Biophysics, Biochemistry

Key words: Cell motility, myosin, actin, biochemistry, biophysics, single molecule, spectroscopy, microscopy, fluorescence, nanotechnology.

Description of Research
The goal of our research is to understand the cellular machinery responsible for powering cell movements and shaping the architecture of cells, tissues, and organs. Our discovery-based research focuses on the role of the cytoskeleton, molecular motors, and signaling pathways in powering cell migration, muscle contraction, and the transport of internal cell compartments. The pathways investigated in our laboratory are crucial for several normal and pathological processes, including: cell and tissue development, endocytosis, wound healing, immune response, cardiomyopathies, and metastases of tumors.

Most of our current efforts are focused on investigating cytoskeletal motors (myosin, dynein, and kinesin). These remarkable nano-machines use chemical energy stored in our cells (in the form of ATP) to generate mechanical force and motion. Cytoskeletal motors are the engines that power muscle contraction, cell migration, intracellular transport, cell division, and cell shape. We are determining how these motors work at the molecular level, how they are physically connected to the machinery they are powering, how they are regulated, how they interact with other motors and signaling networks, and how their fundamental biophysical parameters impact cell function. We are using a range of biochemical, cell biological, single-molecule, and other biophysical techniques to better understand these proteins in health and disease.

Lab Personnel

Betsy Buechler, CAMB Student
Michael Greenberg, Post-doctoral
Tianming Lin, Research Specialist
Serapion Pyrpassopoulos, Post-doctoral
Abbey Weith, Post-doctoral
Michael Woody, BMB Student
Allison Zajac, CAMB Student

Description of Itmat Expertise

Cell Motility
Cytoskeleton
Molecular Mechanics
Molecular Motors
Membrane trafficking
Muscle Biology

Selected Publications

S. Ayloo, J.E. Lazarus, A. Dodda, M. Tokito, E.M. Ostap, E.L.F. Holzbaur: Dynactin functions as both a dynamic tether and brake during dynein-driven motility. Nat Commun 5: 4807, Sep 2014.

H. Shuman, M.J. Greenberg, A. Zwolak, T. Lin, C.V. Sindelar, R. Dominguez, and E.M. Ostap: A Vertebrate Myosin-I Structure Reveals Unique Insights into Myosin Mechanochemical Tuning. PNAS 111(6): 2116-21, Feb 2014.

M.J. Greenberg, H. Shuman, E.M. Ostap: Inherent Force-Dependent Properties of β-Cardiac Myosin Contribute to the Force-Velocity Relationship of Cardiac Muscle. Biophysical Journal In Press, 2014.

A. Zwolak, C. Yang, E.A. Feeser, E.M. Ostap, T. Svitkina, R. Dominguez: CARMIL leading edge localization depends on a non-canonical PH domain and dimerization. Nat Commun 4: 2523, 2013.

A.L. Zajac, Y.E. Goldman, E.L.F. Holzbaur, and E.M. Ostap: Local cytoskeletal and organelle interactions impact molecular motor-driven early endosomal trafficking. Current Biology 23(13): 1173-1180, 2013 Notes: Holzbaur and Ostap are co-corresponding authors.

M. Greenberg, T. Lin, Y.E. Goldman, H. Shuman, E.M. Ostap: Myosin-Ic generates power over a range of loads via a new tension sensing mechanism. PNAS 109(37): E2433-40, August 2012.

S. Pyrpassopoulos, E.A. Feeser, J.N. Mazerik, M.J. Tyska, E.M. Ostap: Membrane-Bound Myo1c Powers Asymmetric Motility of Actin Filaments. Current Biology 22(18): 1688-92, August 2012.

C. Chen, M.J. Greenberg, J.M. Laakso, E.M. Ostap, Y.E. Goldman, H. Shuman: Kinetic schemes for post-synchronized single molecule dynamics. Biophysical Journal 102(6): L23-L25, March 2012.

J. McKenna, Y.E. Goldman, E.M. Ostap: Sites of Glucose Transporter-4 Vesicle Fusion with the Plasma Membrane Correlate Spatially with Microtubules. PloS One 7(8): e43662, 2012.

J.H. Lewis, M.J. Greenberg, J.M. Laakso, H. Shuman, E.M. Ostap: Calcium regulation of myosin-I tension sensing. Biophysical Journal 102(12): 2799-807, 2012.

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Last updated: 11/25/2014
The Trustees of the University of Pennsylvania