Nicolai M Doliba

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Research Assistant Professor of Biochemistry and Biophysics
Senior Research Investigator, Department of Biochemistry and Biophysics and Diabetes Research Center, University of Pennsylvania, Philadelphia
Technical Director of Pancreatic Islet Cell Biology Core, Diabetes Research Center, University of Pennsylvania, Philadelphia
Department: Biochemistry and Biophysics

Contact information
12-131 Smilow Translational Research Center
3400 Civic Center Boulevard
Philadelphia, PA 19104-5160
Office: 215-898-4366
Fax: 215-898-5408
Lab: 215-898-4366
Education:
B.S. (Biology)
Lviv State University, 1982.
Ph.D. (Physiology)
Lviv Medical University, Lviv, Ukraine, 1986.
D.Sc. (Physiology)
Lviv State University, Lviv, Ukraine, 1993.
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Description of Itmat Expertise

My research has focused on the role of bioenergetics, ion transport and metabolic coupling factors in nutrient- and hormone (GLP-1, acetylcholine)-stimulated insulin release in healthy and diabetic islets. For the last 17 years, the availability of human islets from the Islet Resources Center at the University of Pennsylvania provided me with the opportunity to characterize the metabolic, ionic and secretion profiles of normal and T2DM islets.
In addition to my primary interests, as a Technical Director of Islet Cell Biology Core at Diabetes Research Center, I am also involved in various projects of Center Investigators that study normal and abnormal pancreatic beta-cell function.

Description of Research Expertise

My research has focused on the role of bioenergetics, ion transport and metabolic coupling factors in nutrient- and drug-stimulated insulin release at normal conditions and during diabetes mellitus.
Using combinations of different methods and approaches, including 31P, 13C, and 1H Nuclear Magnetic Resonance Spectroscopy techniques, polarographic and phosphorescence methods of measuring O2 consumption, Ca2+ imaging and others, I attempt to directly address the essential mechanisms of stimulus-secretion coupling in pancreatic islets.

Selected Publications

Doliba, Nicolai M. Qin, Wei. Vinogradov, Sergei A. Wilson, David F. Matschinsky, Franz M.: Palmitic acid acutely inhibits acetylcholine- but not GLP-1-stimulated insulin secretion in mouse pancreatic islets. American Journal of Physiology - Endocrinology & Metabolism 299(3): E475-85, Sep 2010.

Gao, Nan. Le Lay, John. Qin, Wei. Doliba, Nicolai. Schug, Jonathan. Fox, Alan J. Smirnova, Olga. Matschinsky, Franz M. Kaestner, Klaus H.: Foxa1 and Foxa2 maintain the metabolic and secretory features of the mature beta-cell. Molecular Endocrinology 24(8): 1594-604, Aug 2010.

Imai, Yumi. Patel, Hiral R. Doliba, Nicolai M. Matschinsky, Franz M. Tobias, John W. Ahima, Rexford S.: Analysis of gene expression in pancreatic islets from diet-induced obese mice. Physiological Genomics 36(1): 43-51, Dec 12 2008.

Imai, Yumi. Patel, Hiral R. Hawkins, Evan J. Doliba, Nicolai M. Matschinsky, Franz M. Ahima, Rexford S.: Insulin secretion is increased in pancreatic islets of neuropeptide Y-deficient mice. Endocrinology 148(12): 5716-23, Dec 2007.

Gao, Nan. White, Peter. Doliba, Nicolai. Golson, Maria L. Matschinsky, Franz M. Kaestner, Klaus H.: Foxa2 controls vesicle docking and insulin secretion in mature Beta cells. Cell Metabolism 6(4): 267-79, Oct 2007.

Doliba, Nicolai M. Wehrli, Suzanne L. Vatamaniuk, Marko Z. Qin, Wei. Buettger, Carol W. Collins, Heather W. Matschinsky, Franz M.: Metabolic and ionic coupling factors in amino acid-stimulated insulin release in pancreatic beta-HC9 cells. American Journal of Physiology - Endocrinology & Metabolism 292(6): E1507-19, Jun 2007.

Hardy, Olga T. Hohmeier, Hans E. Becker, Thomas C. Manduchi, Elisabetta. Doliba, Nicolai M. Gupta, Rana K. White, Peter. Stoeckert, Christian J Jr. Matschinsky, Franz M. Newgard, Christopher B. Kaestner, Klaus H.: Functional genomics of the beta-cell: short-chain 3-hydroxyacyl-coenzyme A dehydrogenase regulates insulin secretion independent of K+ currents. Molecular Endocrinology 21(3): 765-73, Mar 2007.

Vatamaniuk, Marko Z. Gupta, Rana K. Lantz, Kristen A. Doliba, Nicolai M. Matschinsky, Franz M. Kaestner, Klaus H.: Foxa1-deficient mice exhibit impaired insulin secretion due to uncoupled oxidative phosphorylation. Diabetes 55(10): 2730-6, Oct 2006.

Doliba, Nicolai M. Qin, Wei. Vatamaniuk, Marko Z. Buettger, Carol W. Collins, Heather W. Magnuson, Mark A. Kaestner, Klaus H. Wilson, David F. Carr, Richard D. Matschinsky, Franz M.: Cholinergic regulation of fuel-induced hormone secretion and respiration of SUR1-/- mouse islets. American Journal of Physiology - Endocrinology & Metabolism 291(3): E525-35, Sep 2006.

Doliba, Nicolai M. Qin, Wei. Vatamaniuk, Marko Z. Li, Changhong. Zelent, Dorothy. Najafi, Habiba. Buettger, Carol W. Collins, Heather W. Carr, Richard D. Magnuson, Mark A. Matschinsky, Franz M.: Restitution of defective glucose-stimulated insulin release of sulfonylurea type 1 receptor knockout mice by acetylcholine. American Journal of Physiology - Endocrinology & Metabolism 286(5): E834-43, May 2004.

Doliba, Nicolai M. Vatamaniuk, Marko Z. Buettger, Carol W. Qin, Wei. Collins, Heather W. Wehrli, Suzanne L. Carr, Richard D. Matschinsky, Franz M.: Differential effects of glucose and glyburide on energetics and Na+ levels of betaHC9 cells: nuclear magnetic resonance spectroscopy and respirometry studies. Diabetes 52(2): 394-402, Feb 2003.

Doliba NM and Matschinsky FM: Metabolic basis of insulin secretion. In: Contemporary Endocrinology: Type I Diabetes: Etiology and Treatment. M.A. Sperling (eds.). Humana Press Inc., Totowa, NJ, Page: p.115-144, 2003.

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Last updated: 10/18/2017
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