Stella T Chou

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Assistant Professor of Pediatrics at the Children's Hospital of Philadelphia
Attending Physician, Division of Hematology, The Children's Hospital of Philadelphia
Department: Pediatrics

Contact information
3615 Civic Center Boulevard
Abramson Research Building, Room 316D
Philadelphia, PA 19104
Office: 215-590-0947
Fax: 215-590-4834
Education:
B.A. (History)
Duke University, 1995.
M.D.
New York Medical College, 2000.
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Description of Research Expertise

My clinical and research interests are in Pediatric Hematology and Transfusion Medicine. The focus of our laboratory is to investigate mechanisms of normal hematopoietic development and how these become disrupted in hematologic diseases. Our laboratory primarily uses induced pluripotent stem cells (iPSCs) and primary human cells to model blood development and study the pathophysiology of hematopoietic disorders. We specifically focus on erythro-megakaryocytic development and the role of genetic modifiers in anemia, thrombocytopenia, and myeloproliferative disorders. We examine how mutations or gene dosage of transcription factors affect blood development, including understanding the role of trisomy 21 (Down syndrome) and mutated transcription factor GATA-1 in hematologic disorders.

Another focus of our laboratory aims to improve red blood cell (RBC) therapy for patients with sickle cell disease (SCD) using a molecular approach. We showed that Rh alloimmunization in patients with SCD remains a significant problem due to inheritance of variant RH, despite providing Rh-matched transfusions. Current work examines whether genetically matched blood at RH and other blood group loci is feasible for patients with SCD. We are exploring NGS based methods to identify RH variants to increase accessibility. Our laboratory is creating customized iPSCs with rare blood group antigen combinations as renewable sources of RBC reagents to improve antibody identification in patients with SCD. Ultimately, these same cells will serve as proof of principal for a universal donor red cell product generated from iPSCs.

Description of Itmat Expertise

Hematopoiesis
Leukemia
Sickle Cell Disease

Selected Publications

Chou Stella T, Flanagan Jonathan M, Vege Sunitha, Luban Naomi L.C., Brown R. Clark, Ware Russell E, Westhoff Connie M. : Whole exome sequencing for RH genotyping and alloimmunization risk in children with sickle cell anemia. Blood Advances 1(18): 1414-1421, August 2017.

Byrska-Bishop Marta, VanDorn Daniel, Campbell Amy E, Betensky Marisol, Arca Philip R, Yao Yu, Gadue Paul, Costa Fernando F, Nemiroff Richard L, Blobel Gerd A, French Deborah L, Hardison Ross C, Weiss Mitchell J, Chou Stella T: Pluripotent stem cells reveal erythroid-specific activities of the GATA1 N-terminus. The Journal of clinical investigation 125(3): 993-1005, Jan 2015.

Giani Felix C, Fiorini Claudia, Wakabayashi Aoi, Ludwig Leif S, Salem Rany M, Jobaliya Chintan D, Regan Stephanie N, Ulirsch Jacob C, Liang Ge, Steinberg-Shemer Orna, Guo Michael H, Esko T├Ánu, Tong Wei, Brugnara Carlo, Hirschhorn Joel N, Weiss Mitchell J, Zon Leonard I, Chou Stella T, French Deborah L, Musunuru Kiran, Sankaran Vijay G: Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells. Cell Stem Cell 18(1): 73- Jan 2016.

Casas Jessica, Friedman David F, Jackson Tannoa, Vege Sunitha, Westhoff Connie M, Chou Stella T: Changing practice: red blood cell typing by molecular methods for patients with sickle cell disease. Transfusion 55(6 Pt 2): 1388-93, Jun 2015.

Chou Stella T, Jackson Tannoa, Vege Sunitha, Smith-Whitley Kim, Friedman David F, Westhoff Connie M: High prevalence of red blood cell alloimmunization in sickle cell disease despite transfusion from Rh-matched minority donors. Blood 122(6): 1062-71, May 2013.

*Zaslavsky Alexander, *Chou Stella T (*co-first authors), Schadler Keri, Lieberman Allyson, Pimkin Maxim, Kim Yeo Jung, Baek Kwan-Hyuck, Aird William C, Weiss Mitchell J, Ryeom Sandra: The calcineurin-NFAT pathway negatively regulates megakaryopoiesis. Blood 121(16): 3205-15, Apr 2013.

Chou Stella T, Byrska-Bishop Marta, Tober Joanna M, Yao Yu, Vandorn Daniel, Opalinska Joanna B, Mills Jason A, Choi John Kim, Speck Nancy A, Gadue Paul, Hardison Ross C, Nemiroff Richard L, French Deborah L, Weiss Mitchell J: Trisomy 21-associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells. Proceedings of the National Academy of Sciences of the United States of America 109(43): 17573-8, Oct 2012.

Roy Anindita, Cowan Gillian, Mead Adam J, Filippi Sarah, Bohn Georg, Chaidos Aristeidis, Tunstall Oliver, Chan Jerry K Y, Choolani Mahesh, Bennett Phillip, Kumar Sailesh, Atkinson Deborah, Wyatt-Ashmead Josephine, Hu Ming, Stumpf Michael P H, Goudevenou Katerina, O'Connor David, Chou Stella T, Weiss Mitchell J, Karadimitris Anastasios, Jacobsen Sten Eirik, Vyas Paresh, Roberts Irene: Perturbation of fetal liver hematopoietic stem and progenitor cell development by trisomy 21. Proceedings of the National Academy of Sciences of the United States of America 109(43): 17579-84, Oct 2012.

Chou Stella T, Khandros Eugene, Bailey L Charles, Nichols Kim E, Vakoc Christopher R, Yao Yu, Huang Zan, Crispino John D, Hardison Ross C, Blobel Gerd A, Weiss Mitchell J: Graded repression of PU.1/Sfpi1 gene transcription by GATA factors regulates hematopoietic cell fate. Blood 114(5): 983-94, Jul 2009.

Chou Stella T, Opalinska Joanna B, Yao Yu, Fernandes Myriam A, Kalota Anna, Brooks John S J, Choi John K, Gewirtz Alan M, Danet-Desnoyers Gwenn-ael, Nemiroff Richard L, Weiss Mitchell J: Trisomy 21 enhances human fetal erythro-megakaryocytic development. Blood 112(12): 4503-6, Dec 2008.

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Last updated: 11/09/2017
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