Changhong Li

faculty photo
Research Assistant Professor of Pediatrics
Department: Pediatrics

Contact information
802B, ARC
3516 Civic Center Blvd.
Philadelphia, PA 19104
Office: (215)5905380
Fax: (215)5901605
Education:
B.A. (Medicine)
ChengDe Medical Collage, CHINA, 1989.
MD, PhD (Internal Medicine, Endocrinology)
Beijing Medical University, China, 1996.
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Description of Itmat Expertise

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.

Description of Research Expertise

Research Interest:

My research mainly focused on fuel metabolism in islets and regulation of insulin secretion in congenital hyperinsulinism (HI), a group of disorders that cause hypoglycemia in infants and children. My work has involved characterizing most of the known disease models of HI, including glutamate dehydrogenase transgenic mice, sulfonylurea receptor 1 knockout mice, and short-chain 3-hydroxy fatty acyl-CoA dehydrogenase knockout mice. Recently, I have extended these studies of animal models to examine the role of amino acids in regulation of insulin and glucagon secretion by islets from normal and type 2 diabetic humans.

Techniques:

Islets function evaluation: 1) Islets isolation and function studies by batch incubation in 96 well “high-throughput” format and by islets perifusions. 2) Measurement of intracellular calcium in perifused whole islets or dispersed islet cell by duel wave length florescence microscopy.
Fuel Metabolism: 1) Stable isotope tracing: 13C and 15N tracing in isolated islets to study metabolic fluxes from glucose or amino acid; 2)ATP and ATP/ADP ratio measurement to evaluate fuel metabolism.
Gene expression: study gene expression by qPCR in whole islets or in purified beta-cell by cell sorting.

Selected Publications

Nicolai M. Doliba, Qin Liu, Changhong Li, Chengyang Liu, Ali Naji, Franz M. Matschinsky: Inhibition of Cholinergic Potentiation of Insulin Secretion from Pancreatic Islets by Chronic Elevation of Glucose and Fatty Acids: Protection by Casein Kinase 2 Inhibitor. Molecular Metabolism August 2017.

Changhong Li, Amanda M. Ackermann, Kara E. Boodhansingh, Tricia R. Bhatti, Chengyang Liu, Jonathan Schug, Nicolai Doliba, Bing Han, Karen E. Cosgrove, Indraneel Banerjee, Franz M. Matschinsky, Itzhak Nissim, Klaus H. Kaestner, Ali Naji, N. Scott Adzick, Mark J. Dunne, Charles A. Stanley and Diva D. De León: Functional and Metabolomic Consequences of ATP-dependent Potassium Channel Inactivation in Human Islets. Diabetes 66(7): 1901-1913, July 2017.

G Li, J Yin, J Fu, L Li, SFA Grant, C Li, M Li, J Mi, S Gao: FGF21 deficiency is associated with childhood obesity, insulin resistance and hypoadiponectinaemia: The BCAMS Study. Diabetes & Metabolism 43(3): 253-260, June 2017.

Aaron R Cox, Carol J Lam, Matthew M Rankin, Jacqueline S Rios, Julia Chavez, Claire W Bonnyman, Kourtney B King, Roger A Wells, Deepti Anthony, Justin X Tu, Jenny J Kim, Changhong Li, Jake A Kushner: Incretin Therapies Do Not Expand β-cell Mass or Alter Pancreatic Histology in Young Male Mice. Endocrinology 158(6): 1701-1714, June 2017.

Qingrong Huang, Shan He, Yuanyuan Tian, Yuting Gu, Pan Chen, Changhong Li, Jiefang Huang, Yongnian Liu, Hongshuang Yu, Min Jin, Shaoyan Hu, Qing Tong, Anqi Ma, Jian Jin, Elizabeth Hexner, Henry Fung, Ran Reshef, Yi Zhang, Yanyun Zhang: Hsp90 inhibition destabilizes Ezh2 protein in alloreactive T cells and reduces graft-versus-host disease in mice. Blood 129(20): 2737-2748, May 2017.

Lujiao Li, Junling Fu, Xin Ting Yu, Ge Li, Lu Xu, Jinghua Yin, Hong Cheng, Dongqing Hou, Xiaoyuan Zhao, Shan Gao, Wenhui Li, Changhong Li, SF Grant, Mingyao Li, Yi Xiao, Jie Mi, Ming Li: Sleep Duration and Cardiometabolic Risk Among Chinese School-aged Children: Do Adipokines Play a Mediating Role? Sleep 40(5): 1-9, March 2017.

Mary Barrosse‐Antle, Chang Su, Pan Chen, Kara E Boodhansingh, Thomas J Smith, Charles A Stanley, Diva D De León, Changhong Li: A severe case of hyperinsulinism due to hemizygous activating mutation of glutamate dehydrogenase. Pediatric Diabetes Page: 1-6, Feb. 2017.

Benjamin N Ediger, Hee-Woong Lim, Christine Juliana, David N Groff, LaQueena T Williams, Giselle Dominguez, Jin-Hua Liu, Brandon L Taylor, Erik R Walp, Vasumathi Kameswaran, Juxiang Yang, Chengyang Liu, Chad S Hunter, Klaus H Kaestner, Ali Naji, Changhong Li, Maike Sander, Roland Stein, Lori Sussel, Kyoung-Jae Won, Catherine Lee May, Doris A Stoffers: LIM domain–binding 1 maintains the terminally differentiated state of pancreatic β cells. The Journal of Clinical Investigation 127(1): 215-229, Jan 2017.

Junling Fu, Cong Hou, Lujiao Li, Dan Feng, Ge Li, Mingyao Li, Changhong Li, Shan Gao, Ming Li: Vitamin D modifies the associations between circulating betatrophin and cardiometabolic risk factors among youths at risk for metabolic syndrome. Cardiovascular Diabetology 15(1): 1-12, Oct 2016.

C Li, P Chen, C Lam, AM Ackermann, MM Rankin, KH Kaestner, CA Stanley, JA Kushner, A Kelly, RC Rubenstein : Knockout & Overexpression of CFTR in Beta-Cells Reveal its Function in Regulation of Insulin Secretion PEDIATRIC PULMONOLOGY 51: 437-438, September 2016.

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Last updated: 08/14/2017
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