Montserrat C Anguera

faculty photo
Assistant Professor, Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine
Member, Center for Research on Reproduction & Women's Health, University of Pennsylvania Perelman School of Medicine
Member, Institute for Regenerative Medicine, University of Pennsylvania
Member, Institute for Immunology, University of Pennsylvania
Member, Epigenetics Institute, University of Pennsylvania

Contact information
Department of Biomedical Sciences
School of Veterinary Medicine
3800 Spruce St., Room 390EB
Philadelphia, PA 19147
Office: 215-898-0567
Fax: 215-573-6810
Lab: 215-898-9541
Graduate Group Affiliations
Education:
B.A (Environmental Chemistry)
University of California, San Diego, 1998.
Ph.D (Biochemistry, Molecular and Cellular Biology)
Cornell University, 2004.
Permanent link
 

Description of Itmat Expertise

Epigenetics
Long noncoding RNAs
X-chromosome Inactivation

Description of Research Expertise

RESEARCH INTERESTS:

The research in the Anguera laboratory focuses on maintenance of X-chromosome Inactivation in the immune system and in stem cells. They also study epigenetic mechanisms involving long noncoding RNAs during early human development and placental progenitors.

RESEARCH AREAS:

Mechanisms of X-chromosome Inactivation:
We are investigating the molecular mechanisms of X-Chromosome Inactivation, and how altered dosage of X-linked genes affects early embryonic development and contributes to sex-biased disease. We focus on the autoimmune disorder lupus, which has a strong female-bias, exhibits overexpression of X-linked immune-related genes, and involves lymphocytes. We study the epigenetic status of the inactive X in female lymphocytes from humans and mice, and have made the remarkable discovery that these cells do not maintain X-Chromosome Inactivation in the same way as other female somatic cells. We were the first to discover that the inactive X has euchromatic features in female lymphocytes, which may explain the female bias in autoimmune disorders such as lupus. We also study the dynamic mechanisms of Xist RNA localization and heterochromatin mark recruitment to the inactive X following lymphocyte activation.

Long noncoding RNAs during early human development:
We are also investigating sex-specific differences during human placental development using in vitro model systems. We discovered a novel X-linked long noncoding RNA specifically expressed in human placental progenitor cells that regulates the innate immune response.

KEY WORDS

X-chromosome Inactivation, Xist RNA, epigenetics, heterochromatin, female-biased autoimmunity, B cell development, B cell quiescence and activation, T cell quiescence and activation.


WEBSITE
http://www.vet.upenn.edu/research/research-laboratories/research-laboratory/anguera-laboratory

Selected Publications

Wang Jianle, Anguera Montserrat C: In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells. Journal of visualized experiments : JoVE(121), Mar 2017.

Penkala Ian, Wang Jianle, Syrett Camille M, Goetzl Laura, López Carolina B, Anguera Montserrat C: lncRHOXF1, a Long Noncoding RNA from the X Chromosome That Suppresses Viral Response Genes during Development of the Early Human Placenta. Molecular and cellular biology 36(12): 1764-75, Jun 2016.

Wang Jianle, Syrett Camille M, Kramer Marianne C, Basu Arindam, Atchison Michael L, Anguera Montserrat C: Unusual maintenance of X chromosome inactivation predisposes female lymphocytes for increased expression from the inactive X. Proceedings of the National Academy of Sciences of the United States of America 113(14): E2029-38, Apr 2016.

Luo Mengcheng, Zhou Jian, Leu N Adrian, Abreu Carla M, Wang Jianle, Anguera Montserrat C, de Rooij Dirk G, Jasin Maria, Wang P Jeremy: Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis. PLoS genetics 11(1): e1004954, Jan 2015.

Lessing D, Anguera MC, Lee JT.: X chromosome inactivation and epigenetic responses to cellular reprogramming. Annu Rev Genomics Hum Genet. 14: 85-110, May 2013.

Anguera Montserrat C, Sadreyev Ruslan, Zhang Zhaoqing, Szanto Attila, Payer Bernhard, Sheridan Steven D, Kwok Showming, Haggarty Stephen J, Sur Mriganka, Alvarez Jason, Gimelbrant Alexander, Mitalipova Maisam, Kirby James E, Lee Jeannie T: Molecular signatures of human induced pluripotent stem cells highlight sex differences and cancer genes. Cell stem cell 11(1): 75-90, Jul 2012.

Anguera Montserrat C, Ma Weiyuan, Clift Danielle, Namekawa Satoshi, Kelleher Raymond J, Lee Jeannie T: Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain. PLoS genetics 7(9): e1002248, Sep 2011.

Kim Daniel H, Jeon Yesu, Anguera Montserrat C, Lee Jeannie T: X-chromosome epigenetic reprogramming in pluripotent stem cells via noncoding genes. Seminars in cell & developmental biology 22(4): 336-42, Jun 2011.

Field Martha S, Anguera Montserrat C, Page Rodney, Stover Patrick J: 5,10-Methenyltetrahydrofolate synthetase activity is increased in tumors and modifies the efficacy of antipurine LY309887. Archives of biochemistry and biophysics 481(2): 145-50, Jan 2009.

Anguera Montserrat C, Liu Matthew, Avruch Joseph, Lee Jeannie T: Characterization of two Mst1-deficient mouse models. Developmental dynamics : an official publication of the American Association of Anatomists 237(11): 3424-34, Nov 2008.

back to top
Last updated: 08/23/2017
The Trustees of the University of Pennsylvania