| Antibody |
Source |
WB dilution |
IF dilution |
IPs |
| cyclin D1 |
Santa Cruz sc-8396 (monoclonal) |
1:300 |
|
|
| cyclin D1 |
UBI 06-137 (polyclonal) |
1:1000 |
|
|
| cyclin E |
Santa Cruz sc-481 |
1:500 |
|
5ug/200ug lysate |
| cdk2 |
UBI #06-505 |
1:1000 |
|
|
| cdk4 |
Santa Cruz sc-260 (polyclonal) |
1:1000 |
|
5ug/300ug lysate |
| cdk4 |
BioSource AHZ0202 (monoclonal) |
1:500-1:1000 |
|
|
| cdk6 |
Santa Cruz sc-177 |
1:500 |
|
|
| p21 |
Santa Cruz sc-6426 |
1:200-1:500 |
|
|
| p27 |
Transduction Lab. K25020 |
1:2500 |
|
|
| p107 |
Santa Cruz sc-318 |
1:500 |
|
|
| pRb |
Zymed 28-007 |
1:100 |
|
|
| pRb |
Oncogene OP-77 |
1:100 |
|
|
| BrdU |
BioDesign M20105S |
|
1:500 |
|
| phosphotyrosine |
Santa Cruz sc-508 |
1:1000 |
|
|
| phosphotyrosine |
UBI #05-321 |
1:1000 |
|
|
| ERK |
Transduction Lab. M12320 |
1:1000 |
|
|
| ERK1 |
Santa Cruz sc-94 |
|
1:100 |
|
| phospho-ERK |
Cell Signaling 9101S |
1:1000 |
1:200 |
|
| MEK1 |
Transduction Lab. M17020 |
1:5000 |
1:100 |
|
| phosphoAKT |
Cell Signaling #9271 |
1:1000 |
|
|
| FAK |
Transduction Lab. F15020 |
1:500 |
|
|
| FAK |
UBI #05-182 |
|
|
3ug/100ug lysate |
| Shc |
UBI #06-203 |
1:1000 |
|
4ug/250ug lysate |
| vinculin |
Sigma VIN-11-5 |
|
1:100 |
|
| rac |
UBI #05-389 |
1:5000 |
|
|
| cdc42 |
Santa Cruz sc-87 |
1:50 |
|
|
| Rho |
Santa Cruz sc-179 |
1:200 |
|
|
| CREB |
Cell Signaling #9192 |
1:200 |
|
|
| phosphoCREB |
UBI #06-519 |
1:1000 |
|
|
