BMB 509 Principles and Applications of Biophysics and Biochemistry

Monday & Wednesday, 1:30 pm to 3:00 pm

1. Introductory kinetics W Jan 23 Gai/Roder Basic principles; diffusion; methods
2. Introductory kinetics M Jan 28 Gai/Roder VH Ch 5.1-5.2
3. Introductory kinetics W Jan 30 Gai/Roder
4. Hydrogen exchange M Feb 4 Englander the methods
5. Protein folding W Feb 6 Englander examples of kinetic approaches
6. Enzyme kinetics M Feb 11 Kallen T- & P-jump
7. Enzyme kinetics W Feb 13 Kallen isotope effects; tracer @ equil.
8. Allostery M Feb 18 Kallen
9. Enzyme catalysis: Principles W Feb 20 Cooperman transition state, intermediates, solvent
10. Enzyme catalysis: Mechanism M Feb 25 Cooperman acid-base; metal; etc.,
11. Molecular dynamics from NMR W Feb 27 Wand Fast
12. Molecular dynamics from NMR M Mar 3 Wand Slow incl. folding
13. Spectrosc. probes of dynamics W Mar 5 Vanderkooi Dynamic info. From IR/Fl
14. Mol.dyn & mech – simulation M Mar 17 Sharp VH Ch. 3.4; MD, QM/MM etc.
15. Mid-Term Examination W Mar 19
16. Membranes M Mar 24 Lemmon/Baumgart
17. Membranes W Mar 26 Lemmon/Baumgart
18. Membranes M Mar 31 Lemmon/Baumgart
19. Carbohydrates W Apr 2 Lemmon
20. Mass spectrometry M Apr 7 Black VH 15; Physical principles; types
21. Protein post-trans mod W Apr 9 Speicher
22. Proteomics M Apr 14 Speicher
23. Signalling W Apr 16 Lemmon principles, flow, coherence, illustr.
24. Single molecule methods M Apr 21 Goldman VH Ch 16 (not great);
25. Single molecule methods W Apr 23 Goldman Small number stats; methods
26. Final Examination M Apr 28