BMB 626 Mass Spectrometry and Proteomics
Mass Spectrometry and Proteomics (BMB 626) 1/2 credit; second half of semester Description: This course will provide a detailed introduction to mass spectrometry and proteomics. The role of mass spectrometry in both characterizing proteins for traditional protein structure-function studies and identification of proteins in proteome studies will be addressed in detail. The focus will be on solving practical research problems and investigation of cancer and other human diseases. Student evaluation – 40% homework and written discussion of research articles, 30% midterm, 30% final exam. Course requirement – Students will be assigned homework for most lectures that will typically require access to the internet. Some home work assignments will involve problem sets and remaining homework assignments will involve reading research articles and answering discussion questions. Course materials – No textbook. Reviews and research articles will be taken from the current scientific literature. Sample Lecture Schedule |
Lecture |
Topic |
1 |
Overview of mass spectrometry (MS) and proteomics |
2 |
MALDI MS and ESI MS |
3 |
Tandem mass spectrometry |
4 |
De Novo sequencing using MS/MS |
5 |
Characterization of posttranslational modifications |
6 |
Application of MS to solving structure-function problems |
. |
Midterm exam |
7 |
Protein profiling using 2-D gels and 2-D DIGE |
8 |
Proteome compositional analysis using LC-MS/MS |
9 |
Protein profiling using MudPIT, ICAT, etc. with stable isotope labels |
10 |
Protein arrays and protein-protein interaction studies |
11 |
Plasma proteomics |
12 |
Proteomics of human disease |
. |
Final Exam – To be determined |