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BMB 554/CHEM 555

BMB554/CHEM555
Course Director: Emmanuel Skordalakes

The Wistar Institute
Room 330
Tel: (215) 495-6884
e-mail: skorda@wistar.org

Lectures will be on Tuesdays and Thursdays 10:30 a.m. - 12:00 p.m.

Lectures from September 11th – October 25th will be held in Towne 309

Lectures from November 1st – December 18th will be held in The Wistar Auditorium or the Wistar Family Room (WFCR)

Text Books
Crystallography Made Crystal Clear, Gale Rhodes, Academic Press. (3rd edition) - Required: Introduction to Protein Structure, Carl Branden & John Tooze, Garland Publishing Inc. -

Recommended: Texts Books on Reserve (Chemistry Library)
Crystal Structure Analysis, A Primer, Jenny P. Glusker & Kenneth N. Trueblood
X-ray Structure Determination, George H. Stout & Lyle H. Jensen
Practical Protein Crystallography, Duncan E. McRee
Crystal Structure Analysis for Chemists and Biologists, Jenny P. Glusker, Mitch Lewis &
Miriam Rossi

Course Outline
The Course will be broken up into three parts:
(1) Principles of X-ray crystallography.
(2) Macromolecular structures.
(3) Student presentations.

Grading will be based on the following: There will be problem sets (10%) and a mid-term exam covering part 1 (30%), and a final exam covering parts 2 and 3 (30%). For part 3 of the course, students will also be required to make a 20-30 minute presentation on a manuscript describing a macromolecular structure of their choice (30%).

Tentative Schedule - September 11, 13, 18, 20, 25, 27; October 2, 4, 9, 11, 16, 18, 23, 25 (Exam)
Lecturer: Emmanuel Skordalakes
(1) Principles of X-ray crystallography. Topics include:
(a) Why Use X-Rays in Structural Biology
(b) X-Ray Diffraction
(c) Preparation of Crystals
(d) Crystal symmetry, and space groups
(e) Data collection
(f) The structure factor and fourier synthesis
(g) The phase problem (Multiple Isomorphous Replacement, Molecular Replacement,
Anomalous dispersion, Multiple Anomalous Dispersion)
(h) Electron density maps
(i) Electron density modification
2
(j) Crystallographic refinement and analysis

(2) Macromolecular structures will be presented by selected faculty members:
November 1: Emmanuel Skordalakes (WFCR)
November 6: Kate Ferguson
November 8: Greg Van Duyne
November 13: Ronen Marmorstein
November 15: Roberto Domiguez (WFCR)
(3) Student Presentations:
November 20, 27, 29; December 4, 6 (WFCR), 11, 18
Students will present a 20-30 min lecture on a manuscript describing a macromolecular structure of their choice. There will be 2 or 3 presentations per day depending on the number of students enrolled.