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CAMB/BIOL 421 Molecular Genetics

Introduction: What is a Gene?
9/6,11      I. Basic concepts                           1-14, 24-28, 30-33                   
9/13         II. Example of globin genes              33-5,128-36,152-55,172-74,190-93,197-99,38-42,137-40,101-07,109-112
9/18, 20   III. Molecular re-evaluations              45-47, 720-21, 211-15, 724-25, 572-82, 688-89, 584-86, 590-92
                                                                

Identifying and analyzing functional genes

9/25, 27   IV. Genetic mapping                       15-16, 400-401, 742-44, 39-40, 56-60, 123-125
10/2, 4     V. Gene cloning strategies              396-401, 524-25, 422-424, 384-85, 745-50                                      
10/9         VI. Physical gene mapping; positional cloning: examples from human disease (P) 8-10, 64-86, 94-96
10/11       VII. Other methods of gene discovery  522-30, 540-42, 544-46, 343-45, 93-94; (P) 318-20, 195-211, 245-255 10/18           VIII. Reverse genetics, testing gene function   (P) 306-09, 310-14, 176, 320-21    
              
Genomes and sequence analysis
10/23       IX. Genomic analysis: methods; bacterial genomes   (P) 1-4, 65-71, 79-81, 182-83,  86-91, 47-59            
10/25       X. Genomic analysis: eukaryotes I   40-45, 60-61, 79-92, 108-09, 61-63, 114-15, 117-22, 552-54, 556-67 10/30,   XI. Genomic analysis: eukaryotes II -- human evolution    (P) 136-39, 145-49                    

          11/1

Regulation of Gene Transcription

11/6         XII. Prokaryotic Gene regulation: transcription and lac repression         398-401, 258-269, 302-320, 207-209
11/8         XIII Prokaryotic gene regulation: positive and negative trasncrip. control   320-323, 287-295, 356-374        
11/13       XIV. Eukaryotic transcription I   610-15, 618-26, 627-32, 641-45, 649-56, 658-63                                    
11/15       XV. Euk. transcription II: chromatin, activation    645-46, 645-48, 738-40, 758-81, 798-807, 809-10           
11/20       XVI. Euk. transcription III: Negative reg.   648-49, 652, 657-58, 808-09, 810-12, 603                                
11/27       XVII. Epigenetic controls   632-35, 781-86, 786-88, 789-91, 819-36   
                             
Other aspects of gene regulation                                                  
11/29       XVIII. RNA processing, alternate splicing  137-40, 669-83, 683-88, 695-97                                             
12/4         XIX. RNA mediated regulation                 338-345           
12/6         XX. RNA localiz. and stability; Translational controls        140-47
The first recitation session) will be held on Monday, September 17th (there will be no session on Sept. 10th. There will be three non-cumulative exams: the first exam will be given in the recitation period on Monday, Oct. 8 and the second exam will be given in the recitation period on Monday  Nov. 12, both from 5:30-7:30 PM. On Oct. 8 and Nov. 12, therefore, attendance is required from 5:30-7:30.  The third exam will be given at the time scheduled for the Final Exam. The final grade is based on the scores of your two best exams.  If you decide to drop the third exam,  you still must attend the lectures in the last third of the course and participate in the recitation discussions. Note: Do not make travel plans for your Xmas break until the “final exam” time slot is announced.  There will be no makeup exams or rescheduled exams. The texts will be R. Lewin's Genes IX (2007) and G. Gibson and S.V. Muse, A Primer of Genome Science (2004). There are also other required readings, which in most cases will be provided to you as Xeroxed copies. The Teaching Assistant for this semester is Claire Marie Filone .  Contact information and office hour times will be announced on the first day of classes.