X-ray Crystallography

X-ray crystallography remains the gold standard for structure determination of biological macromolecules at atomic resolution. State-of-the art methods that record the diffraction of x-rays from crystalline preparations of the macromolecule of interest can be used to determine the electron density of the molecule, from which a three-dimensional model can be obtained. The prerequisite for this experiment are crystals of the molecule of interest that provide the strongest diffraction of x-rays possible.

  • Rigaku MicroMax-007 HF microfocus rotating anode generator equipped with Osmic VariMax mirror optics, a 4-axis goniometer, and a Saturn – 944+ CCD detector. Data are analyzed using the Rigaku Saturn software package, HKL3000, and dTrek. 
  • A second Rigaku MicroMax-007 HF microfocus rotating anode generator equipped with Osmic mirror optics and a MAR Research CCD detector. Both single crystal diffraction instruments are equipped with refrigerated nitrogen gas cooling systems to maintain crystal temperatures at 100K.
  • Rigaku Alchemist Benchtop Liquid Handling System. Originally purchased to generate crystal screen conditions; might have more general use for large-volume pipetting robotics.
  • TTP Labtech Mosquito Crystal 96-well Nanoliter Liquid Handling Robot. The instrument automates the set-up of protein crystallization vapor diffusion experiments and additive screen in a high-throughput format in the 25-1200 nanoliter regime.
MicroMax007

RIGAKU MICROMAX-007

 

MicroMax 007

A SECOND RIGAKU MICROMAX-007

 

Alchemist

RIGAKU ALCHEMIST BENCHTOP LIQUID HANDLING SYSTEM

 

HP LabTech Mosquito

TPP LABTECH MOSQUITO CRYSTAL 96-WELL NANOLITER LIQUID HANDLING ROBOT

Prices for the Penn Community (FY '17)*:

X-RAY

Initial training, $120/day – mandatory for the first three days of use
Instrument use, $40/hour
Staff assistance, $60/hour – assistance with data analysis

MOSQUITO CRYSTALLIZATION ROBOT

Initial training: $50/experiment – mandatory for the first two days of use
Instrument use: $100/tray

 

This suite of instrumentation resides within the Department X-ray Crystallography Facility at G11 Blockley Hall to facilitate this experimental approach here at the Perelman School of Medicine; the Mosquito Liquid Handling Robot resides in 810 Stellar-Chance Laboratory.

X-Ray Crystallography

Contact:

Steve Stayrook

stayrook@mail.med.upenn.edu

Location:

G11 Blockley Hall

Phone:

215-573-9968

Prices for the Penn Community*:

Initial training, $120/day**

Instrument use, $40/hour

Staff assistance, $60/hour

 

 

Mosquito Crystallization Robot

Contact:

Kushol Gupta

kgupta@mail.med.upenn.edu

Location:

810 Stellar-Chance Laboratories

Prices for the Penn Community*:

Initial training: $50/experiment***

Instrument use: $100/tray

 

Download a billing form:

.docx or .pdf

*

includes all schools of Penn as well as HUP, CHOP, and Wistar

**

mandatory for first three days of use

***

mandatory for first two days of use