Electrochemistry (EChem) 

Biophysical and biochemical systems have many natural redox centers such as hemes, flavins, FeS and other metal clusters which exchange electrons with each other and electrodes at characteristic redox midpoint potentials (Em) and may support redox catalytic reactions with substrates. Surface modified gold, platinum and glassy carbon electrodes interact with diffusing or immobilized proteins to display electrical currents as the electrode potential changes in either a continuous sweep (Cyclic Voltammetry or CV) or step-wise progression (Square Wave Voltammetry, SWV). In Spectro-Electrochemistry, UV/Vis spectra monitor color changes and Em of biological cofactors as the electrode is poised at a series selected potentials.

  • Cyclic and Square Wave Voltammetry with scan rates up to 100 V/s are performed on a BAS 100B workstation (Bioanalytical Systems Inc.) Gold, platinum and glassy carbon electrodes are surface modified to interact with proteins and facilitate electron transfer. Proteins can be immobilized on electrodes for higher densities and electrical currents.
  • Spectro-electrochemistry is performed with a Pine Instruments spectro-electrochemical cell coupled to an Ocean Optics fiber optic spectrometer for UV/Vis spectra as a function of poised redox potential.

Prices for the Penn Community* (FY '17):

Initial training: $60 - 1 day training, mandatory for first day of use
Instrument use: $30/day with consultation
 

Both instruments are located within the Department at 1004 Stellar-Chance Laboratory

Contact:

Chris Moser

moserc@mail.med.upenn.edu

 

Location:

1004 Stellar-Chance Laboratories

 

Prices for the Penn Community*:

Initial training: $60, 1 day training**

Instrument use: $30/day with consultation

 

Download a billing form:

.docx or .pdf

*

includes all schools of Penn as well as HUP, CHOP, and Wistar

**

mandatory for first day of use