Size-Exclusion Chromatography in-line with Multi-Angle Light Scattering
(SEC-MALS)

SEC-MALS marries three complementary forms of detection – ultraviolet (UV), refractive (RI), and static laser light scattering – in-line with standard size-exclusion chromatography, providing a robust methods for the determination of molar mass and oligomeric state in solution of macromolecules. This technique is a robust method for validation of sample purity and monodispersity.

  • A standard HPLC assembly with a Wyatt Technology DAWN HELEOS II 18-angle light scattering instrument with in-line quasi-elastic light scattering and Wyatt Technology tREX refractive interferometer. The ASTRA 5 software package operates the instrument. 
Light Scattering Equipment

18-ANGLE LIGHT SCATTERING EQUIPMENT

Prices for the Penn Community* (FY '17):

Initial training: $50/experiment – mandatory for first three uses
Instrument use: $20/hour

 

This instrument is located within the department at 810 Stellar-Chance Laboratory

Contact:

Kushol Gupta

kgupta@mail.med.upenn.edu

Location:

810 Stellar-Chance Laboratories

Prices for the Penn Community*:

Initial training: $50/experiment**

Instrument use: $20/hour

 

Download a billing form:

.docx or .pdf

*

includes all schools of Penn as well as HUP, CHOP, and Wistar

**

mandatory for first three uses