Laser Activated Visible Transient Spectroscopy (TS)

Many biochemically relevant changes take place on the nanosecond to seconds timescale and can be monitored by visible spectroscopy. This includes laser initiated electron transfer, photolysis or temperature jumps that have been used to study charge separation and recombination, catalysis and protein folding.  To be trained in laser experiments, the UPenn EHRS Laser Safety Training must be completed for the user’s safety.

  • A custom constructed instrument for wide spectral coverage at the 30 nsec and slower timescales. A xenon flash probe through split fiber optic cables illuminate sample (pumped) and reference (not pumped) regions of an anaerobic cuvette, pass through an Acton SP-2156 spectrograph to an image on a Princeton Instruments PiMax-3 ICCD camera that take simultaneous exposures of both sample and reference UV-Vis spectra from a 30 nanoseconds to seconds at desired pump/probe delay times set by a Stanford Research System DG 535 digital delay generator. A ThorLabs beam shutter allows the 10 Hz laser rep rate to be lowered to seconds timescales for the slowest experiments.
  • Spectra-Physics Nd:YAG laser, with frequency conversion crystals for ~100 mJ pulses at 532, 355 and 266 nm 10 Hz excitation. 
  • Quanta-Ray INDI Nd:YAG laser with BasiScan Optical Parametric Oscillator provides tens of millijoule nanosecond pulses continuously tunable over a 410 nm to 2.5 um spectral range at 10 Hz.

Prices for the Penn Community* (FY '17):

Initial training: $100, 2 day training

Instrument use: $100/day with consultation

 
 

These instruments are located within the Department at 261 Anatomy-Chemistry Building

Contact:

Chris Moser

moserc@mail.med.upenn.edu

 

Location:

261 Anatomy-Chemistry Building

 

Prices for the Penn Community*:

Initial training: $100, 2 day training

Instrument use: $100/day with consultation

 

Download a billing form:

.docx or .pdf

*

includes all schools of Penn as well as HUP, CHOP, and Wistar