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Assistant Professor of Bioengineering 373 Towne Building Sarkar Lab Web Page: http://www.seas.upenn.edu/~caslab/ |
The Sarkar laboratory utilizes both experimental and computational methods to quantitatively understand fundamental biomolecular (e.g., binding, catalysis) and cellular (e.g., signaling, trafficking, gene regulation) processes. We seek to understand and manipulate the operation of biomolecular networks, and consequently cellular behavior, by integrating novel molecular design strategies into a cell engineering framework. Current applications range from controlling cell fate decisions to engineering novel therapeutic ligands, receptors, and signaling networks to generating molecular devices for use in synthetic biology. Selected Publications: Sarkar*, C.A., I. Dodevski*, M. Kenig, S. Dudli, A. Mohr, E. Hermans, and A. Plückthun (2008) Directed evolution of a G-protein coupled receptor for expression, stability, and binding selectivity. Proceedings of the National Academy of Sciences USA 105:14808-14813. (*contributed equally) Palani, S., and C.A. Sarkar (2008) Positive receptor feedback during lineage commitment can generate ultrasensitivity to ligand and confer robustness to a bistable switch. Biophysical Journal, 95:1575-1589. Kubetzko*, S., C.A. Sarkar*, and A. Plückthun (2005) Protein PEGylation decreases observed target association rates via a dual blocking mechanism. Molecular Pharmacology 68:1439-1454. (*contributed equally) Sarkar, C.A., K. Lowenhaupt, P.J. Wang, T. Horan, and D.A. Lauffenburger (2003) Parsing the effects of binding, signaling, and trafficking on the mitogenic potencies of granulocyte colony-stimulating factor analogues. Biotechnology Progress 19:955-964. Ricci, M.S., C.A. Sarkar, E.M. Fallon, D.A. Lauffenburger, and D.N Brems (2003) pH dependence of structural stability of interleukin-2 and granulocyte colony-stimulating factor. Protein Science 12:1030-1038. Sarkar, C.A., and D.A. Lauffenburger (2003) Cell-level pharmacokinetic model of granulocyte colony-stimulating factor: implications for ligand lifetime and potency in vivo. Molecular Pharmacology 63:147-158. Sarkar, C.A., K. Lowenhaupt, T. Horan, T.C. Boone, B. Tidor, and D.A. Lauffenburger (2002) Rational cytokine design for increased lifetime and enhanced potency using pH-activated ‘histidine switching. Nature Biotechnology 20:908-913.
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