Biochemistry and Molecular Biophysics Graduate Group

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BMB 554 (CHEM 555) - Macromolecular Crystallography Methods

Fall, every year (1/2 semester, 1/2 credit)

Co-Directors:

Ronen Marmorstein, Ph.D.
Room 326, The Wistar Institute
Tel: (215) 898-5006
e-mail: marmor@wistar.org

Emmanuel Skordalakes, Ph.D.
Room 330, The Wistar Institute
Tel: (215) 495-6884
e-mail: skorda@wistar.org

Prerequisites:

Undergraduate calculus and trigonometry

Required Text:

Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology, Bernhard Rupp, Garland Science.

Course Outline and Grading

The course will coverthe principles of X-ray crystalllography. Grading will be based on the following: problem sets (20%), a midterm exam (40%), and a final exam (40%).

September 8, 13, 15, 20, 22, 27 (midterm exam)
October 4, 6, 11 (no class), 13, 18, 20, 25 (final exam)

Lecturer: Ronen Marmorstein (September 8 - 27)

(i) Why use x-rays in structural biology?
(ii) X-ray diffraction
(iii) Preparation of crystals
(iv) Crystal symmetry and space groups.
(v) Data collection

Lecturer: Emmanuel Skordalakes (September 29 - October 25)

(vi) The structure factor and fourier synthesis
(vii) The phase problem (Multiple Isomorphous Replacement, Molecular Replacement, Anamolous dispersion, Multiple Anomalous Dispersion)
(viii) Electron density maps
(ix) Electron density modification
(x) Crystallographic refinement and analysis