Fall, every year (1/2 semester, 1/2 credit)
Ronen Marmorstein, Ph.D.
Room 326, The Wistar Institute
Tel: (215) 898-5006
Emmanuel Skordalakes, Ph.D.
Room 330, The Wistar Institute
Tel: (215) 495-6884
Undergraduate calculus and trigonometry
Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology, Bernhard Rupp, Garland Science.
Course Outline and Grading
The course will coverthe principles of X-ray crystalllography. Grading will be based on the following: problem sets (20%), a midterm exam (40%), and a final exam (40%).
September 8, 13, 15, 20, 22, 27 (midterm exam)
October 4, 6, 11 (no class), 13, 18, 20, 25 (final exam)
Lecturer: Ronen Marmorstein (September 8 - 27)
(i) Why use x-rays in structural biology?
(ii) X-ray diffraction
(iii) Preparation of crystals
(iv) Crystal symmetry and space groups.
(v) Data collection
Lecturer: Emmanuel Skordalakes (September 29 - October 25)
(vi) The structure factor and fourier synthesis
(vii) The phase problem (Multiple Isomorphous Replacement, Molecular Replacement, Anamolous dispersion, Multiple Anomalous Dispersion)
(viii) Electron density maps
(ix) Electron density modification
(x) Crystallographic refinement and analysis