Spring Semester (1/2 semester; 1/2 credit)
Course Director: Brian Salzberg, Ph.D.
Description: This course will provide an introduction to the principles and application of modern optical methods to the investigation of physiological processes. These include optical measurement of membrane potential, fluorescent indicator measurement of intracellular ion concentrations, single molecule fluorescence measurements, TIRF, FRET, LRET, confocal and multi-photon microscopy, and dynamic light scattering. The course will consist of lectures and discussions of original literature. Intended for second year graduate students, but M.D./Ph.D.’s and postdocs are welcome.
Prerequisites: Undergraduate physics; calculus