Biochemistry and Molecular Biophysics Graduate Group
Fall, even numbered years (1/2 semester, 1/2 credit)
Course Director: Roland G. Kallen, M.D., Ph.D.
Description: Students will be assigned problems in a range of topic, including thermodynamics, enzyme kinetics, redox potentials, and will present their answers in class. This course is intended to complement material covered in BMB 508 and 509, providing a rigorous review of quantitative methods.
Text: Principles and Problems in Physical Chemistry for Biochemists: Third Edition 2001
Nicholas C. Price, Raymond A. Dwek, R. G. Ratcliffe, Mark Wormald, University of Oxford, Oxford University Press
Two sessions/week (1.5 hour each). Students will present the answers to problems from the text and a discussion will ensue.
Subjects:
Chemical potentials and calligative properties
Ideal and non-ideal solutions
Ionic strength, activity of waterThermodynamics
i) Enthalpy (PDRW p. 17, glucose oxidation)
- From tables of enthalpy
- From T-dependence of Keq
Entropy
Free Energy (PDRW p. 22,31)
ii) From equilibrium constant
iii) From other reactions (G1P – G6P) (D. p 77)
Determination of equilibrium constants
Chemical kinetics
Single step reactions
Multistep reactions
Coupled reactionsEnzyme kinetics
Single substrate, MM, irreversible-reversible, complications
pH
Temperature
Multi-substrate
Pre-steady state
Near equilibrium (relaxation)
Coupled reactionsPhotometric analysis
Isotopes
Ideal and non-ideal solutions
Evaluation: Students will be evaluated based upon their presentations, discussions and a final exam.