Biochemistry and Molecular Biophysics Graduate Group

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BMB 629 - Quantitative Problems in Biochemistry and Molecular Biophysics

Fall, even numbered years (1/2 semester, 1/2 credit)

Course Director: Roland G. Kallen, M.D., Ph.D.

Description: Students will be assigned problems in a range of topic, including thermodynamics, enzyme kinetics, redox potentials, and will present their answers in class. This course is intended to complement material covered in BMB 508 and 509, providing a rigorous review of quantitative methods.

Text: Principles and Problems in Physical Chemistry for Biochemists: Third Edition 2001
Nicholas C. Price, Raymond A. Dwek, R. G. Ratcliffe, Mark Wormald, University of Oxford, Oxford University Press

Two sessions/week (1.5 hour each). Students will present the answers to problems from the text and a discussion will ensue.

Subjects:

Chemical potentials and calligative properties

Ideal and non-ideal solutions
Ionic strength, activity of water

Thermodynamics

Determination of equilibrium constants

Chemical kinetics

Single step reactions
Multistep reactions
Coupled reactions

Enzyme kinetics

Single substrate, MM, irreversible-reversible, complications
pH
Temperature
Multi-substrate
Pre-steady state
Near equilibrium (relaxation)
Coupled reactions

Photometric analysis

Isotopes

Ideal and non-ideal solutions

Evaluation: Students will be evaluated based upon their presentations, discussions and a final exam.