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Spring, every year
Course Directors:
Greg Van Duyne, Ph.D., vanduyne@mail.med.upenn.edu
Josh Wand, Ph.D., wand@mail.med.upenn.edu
Prerequisites: BMB 508 or permission of instructor
This course introduces fundamental concepts in chemical kinetics and their application to problems in biochemistry such as protein folding and enzymology. There is an emphasis on dynamic processes in proteins and the techniques used to characterize them over a wide range of timescales. The latter half of the course focuses on emerging areas in biochemistry and biophysics including membrane biochemistry, single molecule methods and proteomics with an emphasis on mass spectrometry.
Textbooks:
Cook and Cleland, "Enzyme Kinetics and Mechanism", Garland Publishing
TENTATIVE 2008 COURSE OUTLINE:
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Date |
Topics |
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W, Jan 23 |
Introductory kinetics |
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M, Jan 28 |
Introductory kinetics |
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W, Jan 30 |
Introductory kinetics |
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M, Feb 4 |
Hydrogen exchange |
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W, Feb 6 |
Protein folding |
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M, Feb 11 |
Enzyme kinetics |
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W, Feb 13 |
Enzyme kinetics |
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M, Feb 18 |
Allostery |
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W, Feb 20 |
Enzyme catalysis: Principles |
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M, Feb 25 |
Enzyme catalysis: Mechanism |
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W, Feb 27 |
Molecular dynamics from NMR |
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M, Mar 3 |
Molecular dynamics from NMR |
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W, Mar 5 |
Spectroscopic probes of dynamics |
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M, Mar 17 |
Mol. dyn & mech – simulation |
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W, Mar 19 |
Mid-Term Examination |
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M, Mar 24 |
Carbohydrates |
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W, Mar 26 |
Protein post-trans mod |
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M, Mar 31 |
Membranes |
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W, Apr 2 |
Membranes |
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M, Apr 7 |
Membranes |
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W, Apr 9 |
Mass spectrometry |
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M, Apr 14 |
Proteomics |
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W, Apr 16 |
Signalling |
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M, Apr 21 |
Single molecule methods |
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W, Apr 23 |
Single molecule methods |
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M, Apr 28 |
Final Examination |
