Application of High Resolution NMR Spectroscopy to Problems in Structural Biology (BMB 618)

1/2 semester course, 1/2 credit
Tuesdays 1:00 - 2:30 p.m. & Thursdays 2:00 - 3:30 p.m.
Room 255 Anatomy-Chemistry Bldg.

Course Director: Professor A. Joshua Wand
Email: wand@mail.med.upenn.edu
Tel: 215-573-7288

Sample Schedule

Lecture

Topic

1

Introduction/Basic Concepts

2

Product Operators

3

Superoperators

4

nD NMR

5

Technical Details

6

Technical Details

7

Proteins

8

1H 2D & 3D Library

9

Sequential & MCD Assignment

10

Polarization Transfer/X-resolved

11

Triple resonance

12

Large protein strategies

13

Structure determination

14

Dynamics

Prerequisites: Rudimentary knowledge of NMR and protein structure; Basic matrix algebra and calculus and elementary quantum mechanics

Recommended Text:

J. Cavanagh, W. J. Fairbrother, A. G. Palmer, III, N. Skelton “Protein NMR Spectroscopy”, Academic Press, New York, 1996 ISBN: 0121644901 (more formal/technical)

or

M. Levitt, “Spin Dynamics: Basics of Nuclear Magnetic Resonance”, John Wiley & Sons, 2001 ISBN: 047148922

Examination: Periodic take-home questions/class presentations. Note, there may be a “workshop” scheduled separately during which student presentations will be made.