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MOLECULAR AND BIOPHYSICAL BASIS OF ION CHANNEL DISEASES (BMB 624)Fall Term Fall, even-numbered years (second half course; 1/2 credit) Course Directors: Roland Kallen and Zhe Lu Course Goals: Examine the function of ion channels in normal cellular signaling focusing on channel gating, ion selectivity and pharmacology; review available structural data on channels; investigate examples of mutations in channels that result in inherited diseases. Format: Lecture and discussion. There will be a practice and then exam problem set. Reference Texts for initial lectures: Ion Channels and Disease by Frances M. Ashcroft 2000 Academic Press Ion Channels of Excitable Membranes (3rd Edition) by Bertil Hille 2001 Sinauer Press |
Sample schedule
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Topic |
Lecturer |
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Friday |
Introduction - Studying Ion Channels |
RGK |
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Monday |
Na Channels |
RGK |
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Friday |
Na Channels |
RGK |
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Introduction: Membrane potential & structure; reversal potential; equations (Nernst, GHK, Boltzmann); electrophysiological protocols; data collection and interpretation (simple kinetic schemes). Steady-state activation, inactivation; single channel studies; use of toxins; probes of structure; mutant cycle analysis. Channel structure: current views |
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Monday |
K channels |
ZL |
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Friday |
K channels |
ZL |
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K+ conduction and selectivity, and voltage gating. |
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Monday |
Ca channels |
DR |
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Friday |
No class |
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Monday |
No class |
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Friday |
Thanksgiving vacation |
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Monday |
Ca Channels |
DR |
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Voltage-gated Ca2+ channels: purification/cloning, subunit composition, properties, physiology and CaV-related genetic diseases. Non voltage-gated Ca2+-permeable channels with focus on the TRP family. |
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Friday |
Dec 1 |
Ligand Gated Channels |
JL |
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Monday |
Dec 4 |
Ligand Gated Channels |
JL |
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Neurotransmission, transmitters, acetylcholine receptors, GABA receptors and glutamate receptors. Discussion of two short reviews on acetylcholine receptors and glutamate receptors (Sine and Engel (2006) Nature 440:448; Mayer (2006) Nature 440: 456. Ernst et al. (2005) Mol Pharmacol 68:1291 |
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Friday |
Dec 8 |
Channelopathies |
RGK |
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Monday |
Dec 11 |
Channelopathies |
RGK |
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Periodic paralysis, Long QT syndrome, Epilepsies, Endplate disease, etc. |
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Friday |
Dec 15 |
Problem Set #1 |
RGK |
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Monday |
Dec 18 |
Exam |
RGK |
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