|
Anna S. Kashina
Assistant Professor, Dept of Animal Biology
Cell Biology
and Physiology
Address
143 Rosenthal (Old Vet)
3800 Spruce St
Philadelphia, PA 19104
Office tel.: 215 746-0895
144 Rosenthal (Old Vet)
3800 Spruce St
Philadelphia, PA 19104
Lab tel.: 215 746-0896
Fax: 215 573-5189
E-mail: akashina@vet.upenn.edu
Link(s)
Dr.
Kashina's Vet School Faculty Page
Education
Moscow State University: BS (Biochemistry), 1987.
Moscow State University: MS (Biochemistry), 1987.
Institute for Protein Research: PhD (Cell Biology), 1992.
|
Research Interests
- Protein modifications, mouse genetics, cancer,
cytoskeleton, cardiovascular development, angiogenesis.
Key words: Protein
arginylation, ATE1, arginine transferase.
Search PubMed for articles
Description of Research
The goal of our research is to investigate the
physiological role of a previously uncharacterized posttranslational
modification, protein arginylation. Knockout of the enzyme
responsible for arginylation, ATE1, results embryonic lethality
in mice and multiple defects related to heart development
and blood vessel remodeling (angiogenesis). Our recent work
showed that arginylation regulates many proteins involved
in cytoskeleton, cell motility, signaling, and metabolism,
and uncovered some mechanisms of this regulation.
Our current studies are focused on three major
directions: (1) identification of the ATE1 protein targets
and studying the effect of arginylation on their properties
and functions; (2) studies of the structure and molecular
properties of the mouse ATE1 enzymes; and (3) discovering
the mechanisms and pathways that lead to the global physiological
effects of protein arginylation.
Recent Publications
Karakozova M, Kozak, M, Wong, C. C. L., Bailey,
A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., and Kashina,
A. (2006) Arginylation of Beta Actin Regulates Actin
Cytoskeleton and Cell Motility. Science. On line:
June 22, 2006
Rai R, Mushegian A, Makarova K, Kashina
A. (2006) Molecular dissection of arginyltransferases
guided by similarity to bacterial peptidoglycan synthases.
EMBO Rep. On line: July 7, 2006
R. Rai and A. Kashina (2005).
Identification of mammalian arginyltransferases that modify
a specific subset of protein substrates. Proc. Natl. Acad.
Sci., USA 102:10123-10128 CITED IN: Chemistry comes to the
cell: ASCB 2005 Marshall TW, Cai L, Bear JE. NATURE CHEMICAL
BIOLOGY 2 (3): 119-122 MAR 2006
Kashina AS. (2006). Differential
arginylation of actin isoforms: the mystery of the actin N-terminus.
Trends Cell Biol. 16,610-615, On line: Oct 11, 2006
A. Kashina and V. Rodionov
(2005) Intracellular Organelle Transport: Few Motors, Many
Signals. Trends in Cell Biology 15:396-398
LAB
Rotation Projects
- Role of protein arginylation in the in vivo
and in vitro properties of proteins
- Analysis of cytoskeleton and cell motility
in Ate1 knockout cells
- Analysis of conditional Ate1 knockout phenotypes
Personnel:
- Reena Rai -- Research Specialist
Sougata Saha -- Postdoctoral Fellow
Junling Wang -- Postdoctoral Fellow
Fangliang Zhang -- Postdoctoral Fellow
Dannee Chen -- Research Specialist
Satoshi Kurosaka -- Postdoctoral FellowM
Adrian Leu -- Research Specialist
last updated 8/2007
|
