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Joshua R. Friedman
Assistant
Professor of Pediatrics
Genetics
and Gene Regulation Program
Address
Abramson 1007B
3615 Civic Center Blvd,
Philadelphia, PA 19104
Office tel.: 267-426-0285
Lab tel.: 215-590-2047
Fax: 501-629-0244
E-mail: friedmaj@mail.med.upenn.edu
Link(s)
The
Joseph Stokes, Jr. Research Institute
Education
Harvard University, A. B.(Biochemistry), 1991
University
of Pennsylvania, PH.D. (Cellular and Molecular Biology), 1996
University of Pennsylvania, M.D., 1998
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Research
Interests
- Liver development, biliary disease, biliary
atresia, microRNA
Key
words:Liver development, organogenesis,
pediatrics, transcription factors, microRNA.
Search PubMed for articles
Description
of Research
My research goals center on how the liver and
bile ducts are formed. An understanding of these processes
can help us diagnose and treat disorders of liver development.
The focus of my laboratory is on the control of gene expression
during liver development and disease, particularly by microRNA,
but also by classical transcription factors.
A combination of genetic and biochemical tools
are used to study these areas. Transgenic and knock-out mouse
models are used to study microRNAs and transcription factors
that may play roles in hepatogenesis and bile duct formation.
This is complemented by the use of a cultured cell model of
hepatoblast differentiation into hepatocytes and cholangioctes.
We combine these approaches with high-throughput techniques
to identify the regulatory networks controlling liver development
which are perturbed in liver disease.
We have a special interest in the pediatric disorder biliary
atresia.
This disease is the most common indication for
pediatric liver transplantation. To study biliary atresia,
we have implemented a mouse model of the disease. We have
also established a protocol with the Biliary Atresia Research
Consortium. Both human and animal models are used in the lab
for the study of microRNA in biliary atresia.
Recent
Publications
Friedman JR, Larris B, Le PP, Peiris TH, Arsenlis
A, Schug J, Tobias JW, Kaestner KH, Greenbaum LE: Orthogonal
analysis of C/EBPbeta targets in vivo during liver proliferation.
Proc Natl Acad Sci U S A 101(35): 12986-91, August
2004.
Friedman JR*, Lee CS*, Fulmer JT, Kaestner KH:
The initiation of liver development is dependent on Foxa transcription
factors. Nature 435(7044): 944-7, June 2005. *Joint
first authorship.
Friedman JR, Kaestner KH. The Foxa family of
transcription factors in development and metabolism. Cell
Mol Life Sci. 2006 Oct;63(19-20):2317-2.
Sackett SD, Fulmer JT, Friedman JR, Kaestner
KH. Foxl1-Cre BAC transgenic mice: a new tool for gene ablation
in the gastrointestinal mesenchyme. Genesis. 2007
Jul 27;45(8):518-522.
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Lab
Rotation
Projects
- Analysis of microRNA expression in liver
development
- Analysis and inhibition of microRNA in the
mouse model of biliary atresia
- Analysis of the role of chemokines in the
mouse model of biliary atresia
- Identification of hepatic microRNA target
genes in cultured cells
- Lab
personnel:
- Zankhana Master, Research Technician
Nick Hand, Post-doctoral Fellow
Dan Leung, Post-doctoral Fellow
Jennifer Cohen, Undergraduate
Alyssa Sclafani, Undergraduate
Jessie Seidelman, Undergraduate
last updated 8/2007
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