Weidong Xiao
Associate Professor, Dept of Pediatrics

Gene Therapy and Vaccines Program

Address

303B Abramson Research Center
34th & Civic Center Boulevard
Philadelphia, PA 19104-4399

Office tel.: 215-590-9170
Lab tel.: 267-426-5063
Fax: 215-590-9939
E-mail: wxiao@mail.med.upenn.edu

Education

Xiangtan University, China: BS (Chemistry), 1989.

University of Science and Technology of China: MS (Material Science and Engineering), 1991.

University of North Carolina at Chapel Hill: PhD (Genetics and Molecular Biology), 1996.

Research Interests

• Basic Virology of Adeno-associated virus, Gene transfer Vectors, Hemophilia

Key words: adeno-associated virus, gene transfer, Hemophilia A.

Description of Research

My research has been focused on the basic biology of adeno-associated virus (AAV) and its application as a gene transfer vector for basic research and human gene therapy.

On AAV vector biology, my lab is working on two issues that are critical for the gene therapy field. One is AAV integration frequency, which has implications for AAV vector safety and its feasibility as a vector for stem cells. The other project is to understand AAV transduction mechanisms through tracking the status of AAV DNA genomes.

On AAV vector development, ongoing projects include: 1) improving a recombinant AAV production system, 2) generating novel AAV serotypes using AAV display technology.

In addition, my lab is developing new strategies to deliver factor VIII gene using AAV vectors. Further applications of AAV vectors are also being explored.

Selected Publications

Lu H, Chen L, Wang J, Huack B, Sarkar R, Zhou S, Xu R, Ding Q, Wang X, Wang H, Xiao W. Complete Correction of Hemophilia A with Adeno-Associated Viral Vectors Containing a Full-Size Expression Cassette. Hum Gene Ther. 2008 May 26

Wang J, Xie J, Lu H, Chen L, Hauck B, Samulski RJ, and Xiao W. Existence of transient functional double-stranded DNA intermediates during recombinant AAV transduction. PNAS 104:13104;13109, 2007.

Chen L, Zhu F, Li J, Lu H, Jiang H, Sarkar R, Arruda VR, Wang J, Zhao J, Pierce GF, Ding Q, Wang X, Wang H, Pipe SW, Liu XQ, Xiao X, Camire RM, Xiao W.  The Enhancing Effects of the Light Chain on Heavy Chain Secretion in Split Delivery of Factor VIII Gene. Mol Ther. 2007.

Hauck B, Xu RR, Xie J, Wu W, Ding Q, Sipler M, Wang H, Chen L, Wright JF, Xiao W. Efficient AAV1-AAV2 hybrid vector for gene therapy of hemophilia. Hum Gene Ther. 17:46-54. 2006.

Schuettrumpf J, Zou J, Zhang Y, Schlachterman A, Liu YL, Edmonson S, Xiao W, Arruda VR. The inhibitory effects of anticoagulation on in vivo gene transfer by adeno-associated viral or adenoviral vectors. Mol Ther. 13:88-97. 2006.

Search PubMed for articles

Lab

Rotation Projects

1. Gene therapy for Hemophilia A.
2. Molecular Biology of AAV genomes.
3. AAV vector development.

Lab personnel

Xiao Yang, M.D.
Hui Lu, Ph.D
Jinhui Wang, Ph.D.
Biao Dong, Ph.D.