Frederic Bushman
Professor, Dept of Microbiology

Microbiology, Virology and Parasitology Program

Address

402C Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104-6076

Office tel.: 215 573-8732
Lab tel.: 215 573-8733
Fax: 215 573-4856
E-mail: bushman@mail.med.upenn.edu

Link(s)

Bushman Laboratory Website
Dr. Bushman's Micro Dept. page

Education

Amherst: BA (Biology and English), 1980.

Harvard University: PhD (Cellular and Developmental Biology), 1988.

Research Interests

• Virology, HIV, Poxviruses, systems biology, bioinformatics, replication

Key words: HIV, integration, integrase, poxvirus, DNA modifying enzyme, virology, variola, RNA interference, interferon.

Description of Research

Research in my laboratory focuses on understanding host-virus interactions, with the dual goal of understanding mechanisms and developing inhibitors. We use a combination of methods in our work--where possible, we try to combine high throughput methods such as deep sequencing, high throughput screening, and RNAi, with more traditional methods such as biochemistry and structural biology. Several of our projects focus on the function of viral DNA modifying enzymes, such as the integrase of HIV and the resolvase and topoisomerase of variola (smallpox) virus.

Selected Publications

Ciuffi, M. Llano, E. Poeschla, H. Marshall, C. Hoffman, J. Leipzig, P. Shinn, J. Ecker, F. Bushman. (2005) A role for LEDGF/p75 in targeting HIV DNA Integration. Nat. Med. 11, p. 1287-1289.

K. Perry, Y. Hwang, F. D. Bushman, and G. D. Van Duyne. (2006) Structural basis for specificity in the poxvirus topoisomerase. Mol. Cell, 23, p. 343-354.

Berry, S. Hannenhalli, J. Leipzig, and F. D. Bushman. (2006) Selection of target sites for mobile DNA integration in the human genome. PLoS Comp. Bio., 2, e157.

G. P. Wang, A. Ciuffi, J. Leipzig, C. C. Berry, F. D. Bushman. (2007) HIV integration site selection: analysis by massively parallel pyrosequencing reveals association with epigenetic modifications. Genome Res. 17, 8, 1186-1194.

G. Wang, A. Garrigue, A. Ciuffi, K. Ronen, J. Leipzig, C. C. Berry, C. Legrasle-Peyrou, F. Benjelloun, S. Hacein-Bey-Abina, A. Fischer, M. Cavazzana-Calvo, and F. D. Bushman. (2008) DNA bar coding and pyrosequencing to analyze adverse events in therapeutic gene transfer. Nuc. Acids Res. 36, e49.

Search PubMed for more articles

Lab

Rotation Projects

1. Study of host cell factors important in the early steps of HIV infection.
2. Study of DNA modifying enzymes important in poxvirus replication.

Lab personnel

Staff Scientist
Young Hwang

Post-doctoral researchers
Troy Brady
Gary Wang

Graduate students
Matt Culyba
Heather Marshall
Rithun Mukherjee
Keshet Ronen

Research Assistants
Christian Hoffmann
Nana Minkah

Programmer
Nirav Malani
Scott Sherrill-Mix

Administrative Assistant
Mali Skotheim